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The Fungal and Bacterial Rhizosphere Microbiome Associated With Grapevine Rootstock Genotypes in Mature and Young Vineyards

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The Fungal and Bacterial Rhizosphere Microbiome Associated With Grapevine Rootstock Genotypes in Mature and Young Vineyards

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dc.contributor.author Berlanas Vicente, Carmen es_ES
dc.contributor.author Berbegal Martinez, Monica es_ES
dc.contributor.author Elena-Jiménez, Georgina es_ES
dc.contributor.author Laidani, Meriem es_ES
dc.contributor.author Cibriain, José Félix es_ES
dc.contributor.author Sagües-Sarasa, Ana es_ES
dc.contributor.author Gramaje Pérez, David es_ES
dc.date.accessioned 2020-04-17T12:49:42Z
dc.date.available 2020-04-17T12:49:42Z
dc.date.issued 2019-05-22 es_ES
dc.identifier.issn 1664-302X es_ES
dc.identifier.uri http://hdl.handle.net/10251/140892
dc.description This Document is Protected by copyright and was first published by Frontiers. All rights reserved. it is reproduced with permission. es_ES
dc.description.abstract [EN] The microbiota colonizing the rhizosphere and the endorhizosphere contribute to plant growth, productivity, carbon sequestration, and phytoremediation. Several studies suggested that different plants types and even genotypes of the same plant species harbor partially different microbiomes. Here, we characterize the rhizosphere bacterial and fungal microbiota across five grapevine rootstock genotypes cultivated in the same soil at two vineyards and sampling dates over 2 years by 16S rRNA gene and ITS high-throughput amplicon sequencing. In addition, we use quantitative PCR (qPCR) approach to measure the relative abundance and dynamic changes of fungal pathogens associated with black-foot disease. The objectives were to (1) unravel the effects of rootstock genotype on microbial communities in the rhizosphere of grapevine and (2) to compare the relative abundances of sequence reads and DNA amount of black-foot disease pathogens. Host genetic control of the microbiome was evident in the rhizosphere of the mature vineyard. Microbiome composition also shifted as year of sampling, and fungal diversity varied with sampling moments. Linear discriminant analysis identified specific bacterial (i.e., Bacillus) and fungal (i.e., Glomus) taxa associated with grapevine rootstocks. Host genotype did not predict any summary metrics of rhizosphere alpha- and beta-diversity in the young vineyard. Regarding black-foot associated pathogens, a significant correlation between sequencing reads and qPCR was observed. In conclusion, grapevine rootstock genotypes in the mature vineyard were associated with different rhizosphere microbiomes. The latter could also have been affected by age of the vineyard, soil properties or field management practices. A more comprehensive study is needed to decipher the cause of the rootstock microbiome selection and the mechanisms by which grapevines are able to shape their associated microbial community. Understanding the vast diversity of bacteria and fungi in the rhizosphere and the interactions between microbiota and grapevine will facilitate the development of future strategies for grapevine protection. es_ES
dc.description.sponsorship The research was financially supported by The National Institute for Agricultural and Food Research and Technology (INIA) under the project RTA2015-00015-C02-01. DG was supported by the DOC-INIA program from the INIA, co-funded by the European Social Fund. CB was supported by the FPI-INIA program from the INIA. GE was supported by the Spanish post-doctoral grant Juan de la Cierva-Formación. es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation.ispartof Frontiers in Microbiology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Bacterial and fungal recruitment es_ES
dc.subject Black-foot disease es_ES
dc.subject Microbial ecology es_ES
dc.subject Microbiome es_ES
dc.subject Rhizosphere es_ES
dc.subject Rootstock selection es_ES
dc.subject.classification BOTANICA es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.title The Fungal and Bacterial Rhizosphere Microbiome Associated With Grapevine Rootstock Genotypes in Mature and Young Vineyards es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fmicb.2019.01142 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//RTA2015-00015-C02-01/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Tecnología Nanofotónica - Institut Universitari de Tecnologia Nanofotònica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani es_ES
dc.description.bibliographicCitation Berlanas Vicente, C.; Berbegal Martinez, M.; Elena-Jiménez, G.; Laidani, M.; Cibriain, JF.; Sagües-Sarasa, A.; Gramaje Pérez, D. (2019). The Fungal and Bacterial Rhizosphere Microbiome Associated With Grapevine Rootstock Genotypes in Mature and Young Vineyards. Frontiers in Microbiology. 10:1-16. https://doi.org/10.3389/fmicb.2019.01142 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fmicb.2019.01142 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 16 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 10 es_ES
dc.relation.pasarela S\392061 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Instituto Nacional de Investigaciones Agrarias es_ES
dc.contributor.funder Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria es_ES
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