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Human Mesenchymal Stem Cells Growth and Osteogenic Differentiation on Piezoelectric Poly(vinylidene fluoride) Microsphere Substrates

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Human Mesenchymal Stem Cells Growth and Osteogenic Differentiation on Piezoelectric Poly(vinylidene fluoride) Microsphere Substrates

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dc.contributor.author Sobreiro-Almeida, Rita es_ES
dc.contributor.author Tamaño-Machiavello, María Noel es_ES
dc.contributor.author Carvalho, E.O. es_ES
dc.contributor.author Cordon, Lourdes es_ES
dc.contributor.author Doria, S. es_ES
dc.contributor.author Senent, Leonor es_ES
dc.contributor.author Correia, D. M. es_ES
dc.contributor.author Ribeiro, C. es_ES
dc.contributor.author Lanceros-Méndez, S. es_ES
dc.contributor.author Sabater i Serra, Roser es_ES
dc.contributor.author Gómez Ribelles, José Luís es_ES
dc.contributor.author Sempere-Talens, Amparo es_ES
dc.date.accessioned 2020-04-17T12:50:38Z
dc.date.available 2020-04-17T12:50:38Z
dc.date.issued 2017 es_ES
dc.identifier.uri http://hdl.handle.net/10251/140923
dc.description.abstract [EN] The aim of this work was to determine the influence of the biomaterial environment on human mesenchymal stem cell (hMSC) fate when cultured in supports with varying topography. Poly(vinylidene fluoride) (PVDF) culture supports were prepared with structures ranging between 2D and 3D, based on PVDF films on which PVDF microspheres were deposited with varying surface density. Maintenance of multipotentiality when cultured in expansion medium was studied by flow cytometry monitoring the expression of characteristic hMSCs markers, and revealed that cells were losing their characteristic surface markers on these supports. Cell morphology was assessed by scanning electron microscopy (SEM). Alkaline phosphatase activity was also assessed after seven days of culture on expansion medium. On the other hand, osteoblastic differentiation was monitored while culturing in osteogenic medium after cells reached confluence. Osteocalcin immunocytochemistry and alizarin red assays were performed. We show that flow cytometry is a suitable technique for the study of the differentiation of hMSC seeded onto biomaterials, giving a quantitative reliable analysis of hMSC-associated markers. We also show that electrosprayed piezoelectric poly(vinylidene fluoride) is a suitable support for tissue engineering purposes, as hMSCs can proliferate, be viable and undergo osteogenic differentiation when chemically stimulated. es_ES
dc.description.sponsorship The authors thank the Portuguese Foundation for Science and Technology (FCT) for financial support under project PTDC/EEI-SII/5582/2014, Strategic Funding UID/FIS/04650/2013 and grants SFRH/BPD/90870/2012 (C.R.) and SFRH/BPD/121526/2016 (D.M.C). The authors acknowledge funding by the Spanish Ministry of Economy and Competitiveness (MINECO) through the project MAT2016-76039-C4-3-R (AEI/FEDER, UE) and from the Basque Government Industry Department under the ELKARTEK program. JLGR, LC, RSS and AS acknowledge funding by the Conselleria de Educacion, Investigacion, Cultura y Deporte of the Generalitat Valenciana through PROMETEO/2016/063 project. CIBER-BBN is an initiative funded by the VI National R&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development. This work was partially financed with FEDER funds (CIBERONC (CB16/12/00284)). The authors acknowledge the assistance and advice of Electron Microscopy Service of the UPV. es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof International Journal of Molecular Sciences es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Tissue engineering es_ES
dc.subject Bone differentiation es_ES
dc.subject Poly(vinylidene fluoride) es_ES
dc.subject Microspheres es_ES
dc.subject.classification INGENIERIA ELECTRICA es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title Human Mesenchymal Stem Cells Growth and Osteogenic Differentiation on Piezoelectric Poly(vinylidene fluoride) Microsphere Substrates es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/ijms18112391 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//CB16%2F12%2F00284/ES/CANCER/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F5582%2F2001/PT/OS EFEITOS DO PODER E DA DIMENSÃO DOS GRUPOS NA VARIABILIDADE "CROSS-SITUATIONAL"/
dc.relation.projectID info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F121526%2F2016/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT/5876/147414/PT/
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2016%2F063/ES/MEDULA OSEA ARTIFICIAL PARA PERSONALIZAR EL TRATAMIENTO DE PACIENTES DE CANCERES DE SANGRE/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F90870%2F2012/PT/TAILORING ELECTRO-MECHANICALLY ACTIVE MATERIALS FOR TISSUE ENGINEERING APPLICATIONS/
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2016-76039-C4-1-R/ES/BIOMATERIALES PIEZOELECTRICOS PARA LA DIFERENCIACION CELULAR EN INTERFASES CELULA-MATERIAL ELECTRICAMENTE ACTIVAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT/3599-PPCDT/121526/PT/Heterometallic Metal-organic Frameworks: Smart Materials for Advanced Applications/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Eléctrica - Departament d'Enginyeria Elèctrica es_ES
dc.description.bibliographicCitation Sobreiro-Almeida, R.; Tamaño-Machiavello, MN.; Carvalho, E.; Cordon, L.; Doria, S.; Senent, L.; Correia, DM.... (2017). Human Mesenchymal Stem Cells Growth and Osteogenic Differentiation on Piezoelectric Poly(vinylidene fluoride) Microsphere Substrates. International Journal of Molecular Sciences. 18(11):1-17. https://doi.org/10.3390/ijms18112391 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/ijms18112391 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 17 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 18 es_ES
dc.description.issue 11 es_ES
dc.identifier.eissn 1422-0067 es_ES
dc.relation.pasarela S\351094 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Gobierno Vasco/Eusko Jaurlaritza es_ES
dc.contributor.funder Fundação para a Ciência e a Tecnologia, Portugal es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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