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dc.contributor.author | Do Nascimento, Noelle M. | es_ES |
dc.contributor.author | Juste-Dolz, Augusto Miguel | es_ES |
dc.contributor.author | Bueno, Paulo Roberto | es_ES |
dc.contributor.author | Monzó, Isidro S. | es_ES |
dc.contributor.author | Tejero, R. | es_ES |
dc.contributor.author | López-Paz, José Luis | es_ES |
dc.contributor.author | Maquieira Catala, Angel | es_ES |
dc.contributor.author | Morais, Sergi | es_ES |
dc.contributor.author | Giménez-Romero, David | es_ES |
dc.date.accessioned | 2020-04-29T07:04:01Z | |
dc.date.available | 2020-04-29T07:04:01Z | |
dc.date.issued | 2018-01 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/141937 | |
dc.description.abstract | [EN] Protein-protein interactions are key in virtually all biological processes. The study of these interactions and the interfaces that mediate them play a key role in the understanding of biological function. In particular, the observation of protein¿protein interactions in their dynamic environment is technically difficult. Here two surface analysis techniques, dual polarization interferometry and quartz crystal microbalance with dissipation monitoring, were paired for real-time mapping of the conformational dynamics of protein¿ protein interactions. Our approach monitors this dynamics in real time and in situ, which is a great advancement within technological platforms for drug discovery. Results agree with the experimental observations of the interaction between the TRIM21a protein and circulating autoantibodies via a bridging bipolar mechanism. This work provides a new chip-based method to monitor conformational dynamics of protein¿protein interactions, which is amenable to miniaturized high-throughput determination. | es_ES |
dc.description.sponsorship | Financial support from the Generalitat Valenciana (GVA-PROMETEO/2014/040), the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund (CTQ2013-45875-R and CTQ2013-42914-R) is acknowledged. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | The Royal Society of Chemistry | es_ES |
dc.relation.ispartof | RSC Advances | es_ES |
dc.rights | Reconocimiento - No comercial (by-nc) | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.title | Mapping molecular binding by means of conformational dynamics measurements | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1039/c7ra10617c | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//CTQ2013-42914-R/ES/SERODIAGNOSTICO DE ENFERMEDADES AUTOINMUNES A TRAVES DE LA RED IDIOTIPO-ANTIIDIOTIPO. BASES Y APLICACION/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F040/ES/Estudio de estrategias fisico-químicas para el desarrollo de biosensores interferométricos en soportes interactivos de aplicación en clínica/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//CTQ2013-45875-R/ES/BIOSENSADO EN SOPORTES INTERACTIVOS CON PROPIEDADES INTERFEROMETRICAS PARA APLICACIONES CLINICAS. DEMOSTRACION EN FARMACOGENETICA Y ALERGIAS MEDICAMENTOSAS/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic | es_ES |
dc.description.bibliographicCitation | Do Nascimento, NM.; Juste-Dolz, AM.; Bueno, PR.; Monzó, IS.; Tejero, R.; López-Paz, JL.; Maquieira Catala, A.... (2018). Mapping molecular binding by means of conformational dynamics measurements. RSC Advances. 8(2):867-876. https://doi.org/10.1039/c7ra10617c | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1039/c7ra10617c | es_ES |
dc.description.upvformatpinicio | 867 | es_ES |
dc.description.upvformatpfin | 876 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 8 | es_ES |
dc.description.issue | 2 | es_ES |
dc.identifier.eissn | 2046-2069 | es_ES |
dc.relation.pasarela | S\350097 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |
dc.contributor.funder | Ministerio de Economía, Industria y Competitividad | es_ES |
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