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Detection, molecular characterisation and aspects involving the transmission of tomato chlorotic dwarf viroid in eggplant

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Detection, molecular characterisation and aspects involving the transmission of tomato chlorotic dwarf viroid in eggplant

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dc.contributor.author Gramazio, Pietro es_ES
dc.contributor.author Lerma Lerma, María Dolores es_ES
dc.contributor.author Villanueva-Párraga, Gloria es_ES
dc.contributor.author Vilanova Navarro, Santiago es_ES
dc.contributor.author García-Fortea, Edgar es_ES
dc.contributor.author Mangino, Giulio es_ES
dc.contributor.author Figás-Moreno, María Del Rosario es_ES
dc.contributor.author Arrones-Olmo, Andrea es_ES
dc.contributor.author Alonso-Martín, David es_ES
dc.contributor.author San Bautista Primo, Alberto es_ES
dc.contributor.author Soler Aleixandre, Salvador es_ES
dc.contributor.author Prohens Tomás, Jaime es_ES
dc.contributor.author Plazas Ávila, María de la O es_ES
dc.date.accessioned 2020-05-07T05:57:02Z
dc.date.available 2020-05-07T05:57:02Z
dc.date.issued 2019-09 es_ES
dc.identifier.issn 0003-4746 es_ES
dc.identifier.uri http://hdl.handle.net/10251/142679
dc.description "This is the peer reviewed version of the following article: Gramazio P, Lerma MD, Villanueva G, et al. Detection, molecular characterisation and aspects involving the transmission of tomato chlorotic dwarf viroid in eggplant. Ann Appl Biol. 2019;175:172 183. https://doi.org/10.1111/aab.12527, which has been published in final form at https://doi.org/10.1111/aab.12527. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving." es_ES
dc.description.abstract [EN] Tomato chlorotic dwarf viroid (TCDVd) is a pospiviroid that causes severe disease symptoms in tomato. TCDVd is also naturally found in other crops and plants, in most occasions being asymptomatic. Apart from the natural hosts reported up to now, artificial inoculations have revealed that TCDVd can infect other plants, including eggplant (Solanum melongena). In a screening of seedlings of eggplant from a breeding programme we detected a pospiviroid, which we identified as TCDVd, representing the first report of natural infection of eggplant by TCDVd. The new TCDVd isolate of eggplant was detected by reverse transcription polymerase chain reaction (RT¿PCR) using primers TG21/CT20, initially designed to detect potato spindle tuber viroid. The new isolate sequence is close to a Brugmansia sanguinea isolate of TCDVd from the Netherlands, and most of the nucleotidic changes with respect to this isolate and to the reference genome sequence of TCDVd are found in the TR region. Naturally infected plants of eggplant with this TCDVd isolate did not display any disease symptoms. We demonstrated that in eggplant TCDVd is mechanically transmitted with low to moderate efficiency with cultivation practices, but not by plant¿to¿plant contact. Tomato plants artificially inoculated with the eggplant isolate of TCDVd tested positive for the presence of the viroid at 50¿days after inoculation, but did not display any disease symptoms. Seed transmission to germinated seedlings of eggplant was variable among progenies from infected plants, ranging from 7.7% to 100.0%. Disinfection of seeds with chemical treatments with sodium hypochlorite and trisodium phosphate solutions plus thermotherapy at 80°C for 24¿hr or 90°C for 6 hr was ineffective in reducing the rate of transmission by seed. We did not find evidence of horizontal transmission of TCDVd by pollen, but vertical transmission was highly efficient when healthy eggplant plants were pollinated with infected pollen. Our results indicate that asymptomatic infection of eggplant by TCDVd and high seed and pollen transmission rates may contribute to the spread of this viroid. The information we obtained is useful in order to implement measures for the prevention, control and eradication of TCDVd in eggplant crops, as well as to avoid their transmission to other hosts. es_ES
dc.description.sponsorship P.G. is grateful to Universitat Politècnica de València and to Japan Society for the Promotion of Science for their respective postdoctoral grants (PAID-10-18 and FY2019 JSPS Postdoctoral Fellowship for Research in Japan [Standard]). A.A. and D.A. are grateful to Universitat Politècnica de València for their respective predoctoral (PAID-01-18 and PAID-01-16) contracts within the Programa de Ayudas de Investigación y Desarrollo initiative. E.G.-F. is grateful to Ministerio de Educación, Cultura y Deporte para la Formación de Profesorado Universitario for a predoctoral grant (FPU17/02389). G.M. is grateful to Generalitat Valenciana for a predoctoral grant within the Santiago Grisolía programme (GRISOLIAP/2016/012). M.P. is grateful to Generalitat Valenciana and Fondo Social Europeo for a postdoctoral grant (APOSTD/2018/014). es_ES
dc.language Inglés es_ES
dc.publisher Blackwell Publishing es_ES
dc.relation JSPS/FY2019 es_ES
dc.relation UPV/PAID-01-18 es_ES
dc.relation UPV/PAID-10-18 es_ES
dc.relation UPV/PAID-01-16 es_ES
dc.relation GV/GRISOLIA/2016/012 es_ES
dc.relation GV/APOSTD/2018/014 es_ES
dc.relation MECD/FPU17/02389 es_ES
dc.relation.ispartof Annals of Applied Biology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Mechanical transmission es_ES
dc.subject Pollen transmission es_ES
dc.subject RT-PCR,seed disinfection es_ES
dc.subject Seed transmission es_ES
dc.subject Solanum melongena es_ES
dc.subject TCDVd es_ES
dc.subject Viroid sequence es_ES
dc.subject.classification GENETICA es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.title Detection, molecular characterisation and aspects involving the transmission of tomato chlorotic dwarf viroid in eggplant es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/aab.12527 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Producción Vegetal - Departament de Producció Vegetal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Gramazio, P.; Lerma Lerma, MD.; Villanueva-Párraga, G.; Vilanova Navarro, S.; García-Fortea, E.; Mangino, G.; Figás-Moreno, MDR.... (2019). Detection, molecular characterisation and aspects involving the transmission of tomato chlorotic dwarf viroid in eggplant. Annals of Applied Biology. 175(2):172-183. https://doi.org/10.1111/aab.12527 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1111/aab.12527 es_ES
dc.description.upvformatpinicio 172 es_ES
dc.description.upvformatpfin 183 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 175 es_ES
dc.description.issue 2 es_ES
dc.relation.pasarela S\407110 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
dc.contributor.funder Japan Society for the Promotion of Science es_ES
dc.contributor.funder Ministerio de Educación, Cultura y Deporte es_ES
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