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Paracrine Anti-inflammatory Effects of Adipose Tissue-Derived Mesenchymal Stem Cells in Human Monocytes

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Paracrine Anti-inflammatory Effects of Adipose Tissue-Derived Mesenchymal Stem Cells in Human Monocytes

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dc.contributor.author Guillen Salazar, Mª Isabel es_ES
dc.contributor.author Platas, Julia es_ES
dc.contributor.author Perez del Caz, M.D. es_ES
dc.contributor.author Mirabet, Vicente es_ES
dc.contributor.author Alcaraz Tormo, Mª Jose es_ES
dc.date.accessioned 2020-05-14T03:04:36Z
dc.date.available 2020-05-14T03:04:36Z
dc.date.issued 2018 es_ES
dc.identifier.issn 1664-042X es_ES
dc.identifier.uri http://hdl.handle.net/10251/143129
dc.description.abstract [EN] The inflammatory process is an essential phenomenon in the induction of immune responses. Monocytes are key effector cells during the inflammatory process. A wide range of evidence indicates that mesenchymal stem cells from adipose tissue (ASC) are endowed with immunomodulatory capacity. However, the interaction between ASC and monocytes in the innate immune response is not well understood. The aim of this work was to investigate the possible paracrine anti-inflammatory effects of ASC in human monocytes. Monocytes were isolated from buffy coats and ASC from fat of non-obese patients. Conditioned medium (CM) from ASC in primary culture was used. We have assessed the effects of CM on the production of inflammatory mediators, degranulation, migration, phagocytic activity, senescence, oxidative stress, mitochondrial membrane potential and macrophage polarization. We have shown that ASC exert paracrine anti-inflammatory actions on human monocytes. CM significantly reduced the production of TNF alpha, NO and PGE2 and the activation of NF-kappa B. In addition, we observed a significant reduction of degranulation, phagocytic activity and their migratory ability in the presence of the chemokine CCL2. The senescence process and the production of oxidative stress and mitochondrial dysfunction were inhibited by CM which also reduced the production of TNF alpha by M1 macrophages while enhanced TGF beta 1 and IL-10 release by M2 macrophages. This study have demonstrated relevant interactions of ASC with human monocytes and macrophages which are key players of the innate immune response. Our results indicate that ASC secretome mediates the anti-inflammatory actions of these cells. This paracrine mechanism would limit the duration and amplitude of the inflammatory response. es_ES
dc.description.sponsorship This work has been funded by grants SAF2017-85806-R (MINECO and FEDER), PROMETEOII/2014/071 (Generalitat Valenciana) and PRCEU-UCH20/11. es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation.ispartof Frontiers in Physiology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Mesenchymal stem cells es_ES
dc.subject Inflammation es_ES
dc.subject Monocytes/macrophages es_ES
dc.subject Oxidative stress es_ES
dc.subject Inflammatory mediators es_ES
dc.title Paracrine Anti-inflammatory Effects of Adipose Tissue-Derived Mesenchymal Stem Cells in Human Monocytes es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fphys.2018.00661 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Universidad CEU Cardenal Herrera//PRCEU-UCH20%2F11/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2017-85806-R/ES/MECANISMOS REGULADORES DE LA INFLAMACION Y SU RESOLUCION EN ENFERMEDADES CRONICAS ARTICULARES Y DE LA PIEL/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F071/ES/Estrategias de protección frente a procesos inflamatorios y degenerativos/ es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Guillen Salazar, MI.; Platas, J.; Perez Del Caz, M.; Mirabet, V.; Alcaraz Tormo, MJ. (2018). Paracrine Anti-inflammatory Effects of Adipose Tissue-Derived Mesenchymal Stem Cells in Human Monocytes. Frontiers in Physiology. 9. https://doi.org/10.3389/fphys.2018.00661 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fphys.2018.00661 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 9 es_ES
dc.identifier.pmid 29904354 es_ES
dc.relation.pasarela S\378196 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Universidad CEU Cardenal Herrera es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
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