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MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit

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MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit

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dc.contributor.author Gil Muñoz, Francisco es_ES
dc.contributor.author SANCHEZ NAVARRO, JESUS ANGEL es_ES
dc.contributor.author Besada Ferreiro, Cristina María es_ES
dc.contributor.author SALVADOR PEREZ, AMPARO ALEJANDRA es_ES
dc.contributor.author BADENES CATALA, MARISA es_ES
dc.contributor.author Naval Merino, María del Mar es_ES
dc.contributor.author RIOS GARCIA, GABINO es_ES
dc.date.accessioned 2020-05-21T03:02:28Z
dc.date.available 2020-05-21T03:02:28Z
dc.date.issued 2020-02-26 es_ES
dc.identifier.issn 2045-2322 es_ES
dc.identifier.uri http://hdl.handle.net/10251/143887
dc.description.abstract [EN] MBW protein complexes containing MYB, bHLH and WD40 repeat factors are known transcriptional regulators of secondary metabolites production such as proanthocyanidins and anthocyanins, and developmental processes such as trichome formation in many plant species. DkMYB2 and DkMYB4 (MYB-type), DkMYC1 (bHLH-type) and DkWDR1 (WD40-type) factors have been proposed by different authors to take part of persimmon MBW complexes for proanthocyanidin accumulation in immature fruit, leading to its characteristic astringent flavour with important agronomical and ecological effects. We have confirmed the nuclear localization of these proteins and their mutual physical interaction by bimolecular fluorescence complementation analysis. In addition, transient expression of DkMYB2, DkMYB4 and DkMYC1 cooperatively increase the expression of a persimmon anthocyanidin reductase gene (ANR), involved in the biosynthesis of cis-flavan-3-ols, the structural units of proanthocyanidin compounds. Collectively, these data support the presence of MBW complexes in persimmon fruit and suggest their coordinated participation in ANR regulation for proanthocyanidin production. es_ES
dc.description.sponsorship This work was funded by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)-FEDER (grant no. RF2013-00043-C02-02 and RTA2017-00011-C03-01). FG-M was funded by a fellowship co-financed by the Generalitat Valenciana and European Social Fund (2014 2020) (grant no. ACIF/2016/115). es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Scientific Reports es_ES
dc.rights Reconocimiento (by) es_ES
dc.title MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/s41598-020-60635-w es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//RF2013-00043-C02-02/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI//RTA2017-00011-C03-01/ES/Mejora de albaricoquero y melocotonero en un contexto de agricultura sostenible y cambio climático/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACIF%2F2016%2F115/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Tecnología de Alimentos - Departament de Tecnologia d'Aliments es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Gil Muñoz, F.; Sanchez Navarro, JA.; Besada Ferreiro, CM.; Salvador Perez, AA.; Badenes Catala, M.; Naval Merino, MDM.; Rios Garcia, G. (2020). MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit. Scientific Reports. 10:1-11. https://doi.org/10.1038/s41598-020-60635-w es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/s41598-020-60635-w es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 11 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 10 es_ES
dc.identifier.pmid 32103143 es_ES
dc.identifier.pmcid PMC7044221 es_ES
dc.relation.pasarela S\403825 es_ES
dc.contributor.funder European Social Fund es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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