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beta-Lactam Antibiotics Modify Root Architecture and Indole Glucosinolate Metabolism in Arabidopsis thaliana

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beta-Lactam Antibiotics Modify Root Architecture and Indole Glucosinolate Metabolism in Arabidopsis thaliana

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dc.contributor.author Gudiño, M. es_ES
dc.contributor.author Blanco-Touriñán, Noel es_ES
dc.contributor.author Arbona, V. es_ES
dc.contributor.author Gómez-Cadenas, Aurelio es_ES
dc.contributor.author Blazquez Rodriguez, Miguel Angel es_ES
dc.contributor.author Navarro-García, F. es_ES
dc.date.accessioned 2020-06-02T05:37:44Z
dc.date.available 2020-06-02T05:37:44Z
dc.date.issued 2018-10 es_ES
dc.identifier.issn 0032-0781 es_ES
dc.identifier.uri http://hdl.handle.net/10251/144831
dc.description.abstract [EN] The presence of antibiotics in soils could be due to natural production by soil microorganisms or to the effect of anthropogenic activities. However, the impact of these compounds on plant physiology has not been thoroughly investigated. To evaluate the effect of beta-lactam antibiotics (carbenicillin and penicillin) on the growth and development of Arabidopsis thaliana roots, plants were grown in the presence of different amounts and we found a reduction in root size, an increase in the size of root hairs as well as an abnormal position closer to the tip of the roots. Those phenomena were dependent on the accumulation of both antibiotics inside root tissues and also correlated with a decrease in size of the root apical meristem not related to an alteration in cell division but to a decrease in cell expansion. Using an RNA sequencing analysis, we detected an increase in the expression of genes related to the response to oxidative stress, which would explain the increase in the levels of endogenous reactive oxygen species found in the presence of those antibiotics. Moreover, some auxin-responsive genes were misregulated, especially an induction of CYP79B3, possibly explaining the increase in auxin levels in the presence of carbenicillin and the decrease in the amount of indole glucosinolates, involved in the control of fungal infections. Accordingly, penicillin-treated plants were hypersensitive to the endophyte fungus Colletotrichum tofieldiae. These results underscore the risks for plant growth of beta-lactam antibiotics in agricultural soils, and suggest a possible function for these compounds as fungus-produced signaling molecules to modify plant behavior. es_ES
dc.description.sponsorship This work has been financed by the Spanish Ministerio de Medio Ambiente Rural y Marino [MMA 022/PC08/3-04.2] and the Spanish Agencia Estatal de Investigación [BFU2016-80621-P]. M.E.G. is a fellow of the program Convocatoria Abierta 2013 - Fase I (Instituto de Fomento de Talento Humano (IFTH), Secretaría Nacional de Educación Superior Ciencia y Tecnología (SENESCYT) (Ecuador). N.B-T is a recipient of a MINECO FPI Fellowship (BES-2014-068868). es_ES
dc.language Inglés es_ES
dc.publisher Oxford University Press es_ES
dc.relation.ispartof Plant and Cell Physiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Arabidopsis es_ES
dc.subject Auxin es_ES
dc.subject Beta-lactams es_ES
dc.subject Glucosinolates es_ES
dc.subject Root es_ES
dc.subject ROS es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title beta-Lactam Antibiotics Modify Root Architecture and Indole Glucosinolate Metabolism in Arabidopsis thaliana es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/pcp/pcy128 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BES-2014-068868/ES/BES-2014-068868/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MARM//022%2FPC08%2F3-04.2/ES/Metodologías para la monitorización de la aplicación de lodos de depuradora. Bioseguridad microbiana y modelos de flujo y transporte de contaminantes solubles/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2016-80621-P/ES/ANÁLISIS EVOLUTIVO DE UN 'HUB' FUNCIONAL EN PLANTAS/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Gudiño, M.; Blanco-Touriñán, N.; Arbona, V.; Gómez-Cadenas, A.; Blazquez Rodriguez, MA.; Navarro-García, F. (2018). beta-Lactam Antibiotics Modify Root Architecture and Indole Glucosinolate Metabolism in Arabidopsis thaliana. Plant and Cell Physiology. 59(10):2086-2098. https://doi.org/10.1093/pcp/pcy128 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1093/pcp/pcy128 es_ES
dc.description.upvformatpinicio 2086 es_ES
dc.description.upvformatpfin 2098 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 59 es_ES
dc.description.issue 10 es_ES
dc.identifier.pmid 29986082 es_ES
dc.relation.pasarela S\382350 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Ministerio de Medio Ambiente y Medio Rural y Marino es_ES
dc.contributor.funder Secretaría de Educación Superior, Ciencia, Tecnología e Innovación, Ecuador
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