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Prospective multicenter real-world RAS mutation comparison between OncoBEAM-based liquid biopsy and tissue analysis in metastatic colorectal cancer

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Prospective multicenter real-world RAS mutation comparison between OncoBEAM-based liquid biopsy and tissue analysis in metastatic colorectal cancer

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dc.contributor.author García-Foncillas, J. es_ES
dc.contributor.author Tabernero, J. es_ES
dc.contributor.author Ëlez, E. es_ES
dc.contributor.author Aranda, E. es_ES
dc.contributor.author Benavides, M. es_ES
dc.contributor.author Camps-Herrrero, Carlos es_ES
dc.contributor.author Jantus-Lewintre, Eloisa es_ES
dc.contributor.author Lopez, R. es_ES
dc.contributor.author Muinelo-Romay, L. es_ES
dc.contributor.author Montagut, C. es_ES
dc.contributor.author Antón, A. es_ES
dc.contributor.author Lopez, G. es_ES
dc.contributor.author Diaz-Rubio, E. es_ES
dc.contributor.author Rojo, F. es_ES
dc.contributor.author Vivancos, A. es_ES
dc.date.accessioned 2020-06-12T03:33:30Z
dc.date.available 2020-06-12T03:33:30Z
dc.date.issued 2018-12-11 es_ES
dc.identifier.issn 0007-0920 es_ES
dc.identifier.uri http://hdl.handle.net/10251/146168
dc.description.abstract [EN] BACKGROUND: Liquid biopsy offers a minimally invasive alternative to tissue-based evaluation of mutational status in cancer. The goal of the present study was to evaluate the aggregate performance of OncoBEAM RAS mutation analysis in plasma of colorectal cancer (CRC) patients at 10 hospital laboratories in Spain where this technology is routinely implemented. METHODS: Circulating cell-free DNA from plasma was examined for RAS mutations using the OncoBEAM platform at each hospital laboratory. Results were then compared to those obtained from DNA extracted from tumour tissue from the same patient. RESULTS: The overall percentage agreement between plasma-based and tissue-based RAS mutation testing of the 236 participants was 89% (210/236; kappa, 0.770 (95% CI: 0.689-0.852)). Re-analysis of tissue from all discordant cases by BEAMing revealed two false negative and five false positive tumour tissue RAS results, with a final concordance of 92%. Plasma false negative results were found more frequently in patients with exclusive lung metastatic disease. CONCLUSIONS: In this first prospective real-world RAS mutation performance comparison study, a high overall agreement was observed between results obtained from plasma and tissue samples. Overall, these findings indicate that the plasma-based BEAMing assay is a viable solution for rapid delivery of RAS mutation status to determine mCRC patient eligibility for anti-EGFR therapy. es_ES
dc.description.sponsorship This work was supported by Sysmex Inostics, Inc as well as the AES Programme [Grant Numbers PI15/00934, PT17/0015/0006]. The funders had no involvement in the writing of this manuscript but did review for medical accuracy prior to submission. es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation ISCIII/PI15/00934 es_ES
dc.relation ISCIII/PT17/0015/0006 es_ES
dc.relation.ispartof British Journal of Cancer es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Circulating tumor DNA es_ES
dc.subject Plus cetuximab treatment es_ES
dc.subject Growth-Factor receptor es_ES
dc.subject Anti-EGFR therapy es_ES
dc.subject Clinical-Relevance es_ES
dc.subject Digital PCR es_ES
dc.subject Kras es_ES
dc.subject Folfiri es_ES
dc.subject Braf es_ES
dc.subject Heterogeneity es_ES
dc.subject.classification BIOLOGIA CELULAR es_ES
dc.title Prospective multicenter real-world RAS mutation comparison between OncoBEAM-based liquid biopsy and tissue analysis in metastatic colorectal cancer es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/s41416-018-0293-5 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation García-Foncillas, J.; Tabernero, J.; Ëlez, E.; Aranda, E.; Benavides, M.; Camps-Herrrero, C.; Jantus-Lewintre, E.... (2018). Prospective multicenter real-world RAS mutation comparison between OncoBEAM-based liquid biopsy and tissue analysis in metastatic colorectal cancer. British Journal of Cancer. 119(12):1464-1470. https://doi.org/10.1038/s41416-018-0293-5 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/s41416-018-0293-5 es_ES
dc.description.upvformatpinicio 1464 es_ES
dc.description.upvformatpfin 1470 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 119 es_ES
dc.description.issue 12 es_ES
dc.identifier.pmid 30467411 es_ES
dc.identifier.pmcid PMC6288144 es_ES
dc.relation.pasarela S\388745 es_ES
dc.contributor.funder Sysmex Inostics, Inc. es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
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