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Maintenance of chondrocyte phenotype during expansion on PLLA microtopographies

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Maintenance of chondrocyte phenotype during expansion on PLLA microtopographies

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dc.contributor.author Costa Martínez, Elisa es_ES
dc.contributor.author González García, Cristina es_ES
dc.contributor.author Gómez Ribelles, José Luís es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.date.accessioned 2020-06-16T03:45:48Z
dc.date.available 2020-06-16T03:45:48Z
dc.date.issued 2018-08-06 es_ES
dc.identifier.uri http://hdl.handle.net/10251/146439
dc.description.abstract [EN] Articular chondrocytes are difficult to grow, as they lose their characteristic phenotype following expansion on standard tissue culture plates. Here, we show that culturing them on surfaces of poly(L-lactic acid) of well-defined microtopography allows expansion and maintenance of characteristic chondrogenic markers. We investigated the dynamics of human chondrocyte dedifferentiation on the different poly(L-lactic acid) microtopographies by the expression of collagen type I, collagen type II and aggrecan at different culture times. When seeded on poly(L-lactic acid), chondrocytes maintained their characteristic hyaline phenotype up to 7days, which allowed to expand the initial cell population approximately six times without cell dedifferentiation. Maintenance of cell phenotype was afterwards correlated to cell adhesion on the different substrates. Chondrocytes adhesion occurs via the (51) integrin on poly(L-lactic acid), suggesting cell-fibronectin interactions. However, (21) integrin is mainly expressed on the control substrate after 1day of culture, and the characteristic chondrocytic markers are lost (collagen type II expression is overcome by the synthesis of collagen type I). Expanding chondrocytes on poly(L-lactic acid) might be an effective solution to prevent dedifferentiation and improving the number of cells needed for autologous chondrocyte transplantation. es_ES
dc.description.sponsorship The support received from the European Research Council (ERC 306990) and the UK EPSRC (EP/P001114/1) is acknowledged. J.L.G.R. acknowledges support of the Spanish Ministry of Economy and Competitiveness (MINECO) through the project MAT2016-76039-C4-1 (including the FEDER financial support). CIBER-BBN is an initiative funded by the VI National R&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Programme, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. es_ES
dc.language Inglés es_ES
dc.publisher SAGE Publications es_ES
dc.relation.ispartof Journal of Tissue Engineering es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Poly(L-lactic acid) es_ES
dc.subject Chondrocyte dedifferentiation es_ES
dc.subject Cell adhesion es_ES
dc.subject Integrins es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Maintenance of chondrocyte phenotype during expansion on PLLA microtopographies es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1177/2041731418789829 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/306990/EU/Material-driven Fibronectin Fibrillogenesis to Engineer Synergistic Growth Factor Microenvironments/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UKRI//EP%2FP001114%2F1/GB/Engineering growth factor microenvironments - a new therapeutic paradigm for regenerative medicine/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2016-76039-C4-1-R/ES/Biomateriales piezoeléctricos para la diferenciación celular en interfases célula-material eléctricamente activas/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2016-76039-C4-1-R/ES/BIOMATERIALES PIEZOELECTRICOS PARA LA DIFERENCIACION CELULAR EN INTERFASES CELULA-MATERIAL ELECTRICAMENTE ACTIVAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Costa Martínez, E.; González García, C.; Gómez Ribelles, JL.; Salmerón Sánchez, M. (2018). Maintenance of chondrocyte phenotype during expansion on PLLA microtopographies. Journal of Tissue Engineering. 9. https://doi.org/10.1177/2041731418789829 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1177/2041731418789829 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 9 es_ES
dc.identifier.eissn 2041-7314 es_ES
dc.identifier.pmid 30093985 es_ES
dc.identifier.pmcid PMC6080075 es_ES
dc.relation.pasarela S\382707 es_ES
dc.contributor.funder UK Research and Innovation es_ES
dc.contributor.funder Engineering and Physical Sciences Research Council, Reino Unido es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina es_ES
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