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Transcriptomic changes in Cucurbita pepo fruit after cold storage: differential response between two cultivars contrasting in chilling sensitivity

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Transcriptomic changes in Cucurbita pepo fruit after cold storage: differential response between two cultivars contrasting in chilling sensitivity

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Nombre: Carvajal;Rosales;Palma ...
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dc.contributor.author Carvajal, F es_ES
dc.contributor.author Rosales, R es_ES
dc.contributor.author Palma, F es_ES
dc.contributor.author Manzano, S. es_ES
dc.contributor.author Cañizares Sales, Joaquín es_ES
dc.contributor.author Jamilena, M. es_ES
dc.contributor.author Garrido, D. es_ES
dc.date.accessioned 2020-06-17T03:39:08Z
dc.date.available 2020-06-17T03:39:08Z
dc.date.issued 2018-02-07 es_ES
dc.identifier.issn 1471-2164 es_ES
dc.identifier.uri http://hdl.handle.net/10251/146497
dc.description.abstract [EN] Background: Zucchini fruit is susceptible to chilling injury (CI), but the response to low storage temperature is cultivar dependent. Previous reports about the response of zucchini fruit to chilling storage have been focused on the physiology and biochemistry of this process, with little information about the molecular mechanisms underlying it. In this work, we present a comprehensive analysis of transcriptomic changes that take place after cold storage in zucchini fruit of two commercial cultivars with contrasting response to chilling stress. Results: RNA-Seq analysis was conducted in exocarp of fruit at harvest and after 14 days of storage at 4 and 20 degrees C. Differential expressed genes (DEGs) were obtained comparing fruit stored at 4 degrees C with their control at 20 degrees C, and then specific and common up and down-regulated DEGs of each cultivar were identified. Functional analysis of these DEGs identified similarities between the response of zucchini fruit to low temperature and other stresses, with an important number of GO terms related to biotic and abiotic stresses overrepresented in both cultivars. This study also revealed several molecular mechanisms that could be related to chilling tolerance, since they were up-regulated in cv. Natura (CI tolerant) or down-regulated in cv. Sinatra (CI sensitive). These mechanisms were mainly those related to carbohydrate and energy metabolism, transcription, signal transduction, and protein transport and degradation. Among DEGs belonging to these pathways, we selected candidate genes that could regulate or promote chilling tolerance in zucchini fruit including the transcription factors MYB76-like, ZAT10-like, DELLA protein GAIP, and AP2/ERF domain-containing protein. Conclusions: This study provides a broader understanding of the important mechanisms and processes related to coping with low temperature stress in zucchini fruit and allowed the identification of some candidate genes that may be involved in the acquisition of chilling tolerance in this crop. These genes will be the basis of future studies aimed to identify markers involved in cold tolerance and aid in zucchini breeding programs. es_ES
dc.description.sponsorship This research has been funded by the Ministerio de Economia y Competitividad and Fondo Europeo de Desarrollo Regional FEDER (Project AGL2014-54598-C2). es_ES
dc.language Inglés es_ES
dc.publisher Springer (Biomed Central Ltd.) es_ES
dc.relation.ispartof BMC Genomics es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Zucchini fruit es_ES
dc.subject Postharvest physiology es_ES
dc.subject Cold tolerance es_ES
dc.subject Transcriptomic profiling es_ES
dc.subject Stress response es_ES
dc.subject.classification GENETICA es_ES
dc.title Transcriptomic changes in Cucurbita pepo fruit after cold storage: differential response between two cultivars contrasting in chilling sensitivity es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/s12864-018-4500-9 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2014-54598-C2-2-R/ES/DESARROLLO DE HERRAMIENTAS FISIOLOGICAS Y GENOMICAS PARA MEJORAR LA CALIDAD POSTCOSECHA DEL FRUTO DE CALABACIN/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Carvajal, F.; Rosales, R.; Palma, F.; Manzano, S.; Cañizares Sales, J.; Jamilena, M.; Garrido, D. (2018). Transcriptomic changes in Cucurbita pepo fruit after cold storage: differential response between two cultivars contrasting in chilling sensitivity. BMC Genomics. 19. https://doi.org/10.1186/s12864-018-4500-9 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1186/s12864-018-4500-9 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 19 es_ES
dc.identifier.pmid 29415652 es_ES
dc.identifier.pmcid PMC5804050 es_ES
dc.relation.pasarela S\378446 es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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