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Automation of Heterogeneous Enzyme Immunoassay for Atrazine. Comparison of Three Immobilization Supports

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Automation of Heterogeneous Enzyme Immunoassay for Atrazine. Comparison of Three Immobilization Supports

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dc.contributor.author González Martínez, Miguel Ángel es_ES
dc.contributor.author Morais, Sergi es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Maquieira Catala, Angel es_ES
dc.contributor.author Marco, M. es_ES
dc.contributor.author Barceló, D. es_ES
dc.date.accessioned 2020-06-23T03:46:48Z
dc.date.available 2020-06-23T03:46:48Z
dc.date.issued 1998-05 es_ES
dc.identifier.issn 0937-0633 es_ES
dc.identifier.uri http://hdl.handle.net/10251/146777
dc.description.abstract [EN] Intensive agriculture associated with the use of large amounts of different pesticides, together with the growing concern about the potential contamination of ground water, have brought about the need for developing fast screening methods. This work presents the automation of an enzyme-linked immunosorbent assay for atrazine by means of a flow-through system. Three different solid supports for antibody immobilization were compared in a direct competitive assay format. Sensitivity reached in all cases was below the maximum level allowed in the EU (100 ng L-1). Cross-reactivity of atrazine-related compounds was also studied. The performance of the different supports is discussed regarding sensitivity and immunosurface regeneration. es_ES
dc.description.sponsorship This work was supported by the CICYT project AMB 96-1079 and the DGCYT project PB 95-0740, Comission of the European Community (Contract ENV4-CT96-0333). M.A.González-Martínez acknowledges a grant from the Fundación Cajamadrid, Spain, to carry out a PhD degree. es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation CICYT/AMB 96-1079 es_ES
dc.relation EC/ENV4-CT96-0333 es_ES
dc.relation MINISTERIO DE EDUCACION /PB95-0740 es_ES
dc.relation.ispartof Fresenius Journal of Analytical Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Automation of Heterogeneous Enzyme Immunoassay for Atrazine. Comparison of Three Immobilization Supports es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s002160050858 es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation González Martínez, MÁ.; Morais, S.; Puchades, R.; Maquieira Catala, A.; Marco, M.; Barceló, D. (1998). Automation of Heterogeneous Enzyme Immunoassay for Atrazine. Comparison of Three Immobilization Supports. Fresenius Journal of Analytical Chemistry. 361(2):179-184. https://doi.org/10.1007/s002160050858 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/ 10.1007/s002160050858 es_ES
dc.description.upvformatpinicio 179 es_ES
dc.description.upvformatpfin 184 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 361 es_ES
dc.description.issue 2 es_ES
dc.relation.pasarela S\317386 es_ES
dc.contributor.funder CICYT es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder MINISTERIO DE EDUCACION es_ES


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