Pasin, F.; Bedoya, LC.; Bernabé-Orts, JM.; Gallo, A.; Simón-Mateo, C.; Orzáez Calatayud, DV.; García, JA. (2017). Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins. ACS Synthetic Biology. 6(10):1962-1968. https://doi.org/10.1021/acssynbio.6b00354
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/147327
Title:
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Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins
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Author:
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Pasin, Fabio
Bedoya, Leonor C.
Bernabé-Orts, Joan Miquel
Gallo, Araíz
Simón-Mateo, Carmen
Orzáez Calatayud, Diego Vicente
García, Juan Antonio
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UPV Unit:
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Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
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Issued date:
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Abstract:
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[EN] Improved plants are necessary to meet human needs. Agrobacterium-mediated transformation is the most common method used to rewire plant capabilities. For plant gene delivery, DNA constructs are assembled into binary ...[+]
[EN] Improved plants are necessary to meet human needs. Agrobacterium-mediated transformation is the most common method used to rewire plant capabilities. For plant gene delivery, DNA constructs are assembled into binary T-DNA vectors that rely on broad host range origins for bacterial replication. Here we present pLX vectors, a set of mini binary T-DNA plasmids suitable for Type IIS restriction endonuclease- and overlap-based assembly methods. pLX vectors include replicons from compatible broad host range plasmids. Simultaneous usage of pBBR1- and RK2-based pLX vectors in a two-plasmid/one-Agrobacterium strain strategy allowed multigene delivery to plants. Adoption of pLX vectors will facilitate routine plant transformations and targeted mutagenesis, as well as complex part and circuit characterization.
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Subjects:
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Agrobacterium tumefaciens
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Binary T-DNA vector
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Ethanol-inducible switch
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Multigene transfer
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PBBR1
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Plant biotechnology
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Potyviridae
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Ugandan cassava brown streak virus
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Copyrigths:
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Cerrado |
Source:
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ACS Synthetic Biology. (eissn:
2161-5063
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DOI:
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10.1021/acssynbio.6b00354
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Publisher:
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American Chemical Society
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Publisher version:
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http://dx.doi.org/10.1021/acssynbio.6b00354
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Project ID:
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MINECO/BIO2013-49053-R
MINECO/BIO2016-80572-R
MINECO/KBBE PCIN-2013-056
MINECO/BIO2013-42193-R
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Thanks:
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We thank Victor de Lorenzo, Salome Prat, and Stephan Winter for supply of materials. We are grateful to Jose Luis Martinez for use of the Infinite M200 fluorometer, Beatriz Garcia for technical assistance and Catherine ...[+]
We thank Victor de Lorenzo, Salome Prat, and Stephan Winter for supply of materials. We are grateful to Jose Luis Martinez for use of the Infinite M200 fluorometer, Beatriz Garcia for technical assistance and Catherine Mark for manuscript editing. This work was funded by grants BIO2013-49053-R, BIO2013-42193-R, BIO2016-80572-R and Plant KBBE PCIN-2013-056 from the Spanish Ministerio de Ciencia y Economia.
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Type:
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Artículo
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