- -

Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins

Show simple item record

Files in this item

dc.contributor.author Pasin, Fabio es_ES
dc.contributor.author Bedoya, Leonor C. es_ES
dc.contributor.author Bernabé-Orts, Joan Miquel es_ES
dc.contributor.author Gallo, Araíz es_ES
dc.contributor.author Simón-Mateo, Carmen es_ES
dc.contributor.author Orzáez Calatayud, Diego Vicente es_ES
dc.contributor.author García, Juan Antonio es_ES
dc.date.accessioned 2020-07-02T06:51:19Z
dc.date.available 2020-07-02T06:51:19Z
dc.date.issued 2017-10 es_ES
dc.identifier.uri http://hdl.handle.net/10251/147327
dc.description.abstract [EN] Improved plants are necessary to meet human needs. Agrobacterium-mediated transformation is the most common method used to rewire plant capabilities. For plant gene delivery, DNA constructs are assembled into binary T-DNA vectors that rely on broad host range origins for bacterial replication. Here we present pLX vectors, a set of mini binary T-DNA plasmids suitable for Type IIS restriction endonuclease- and overlap-based assembly methods. pLX vectors include replicons from compatible broad host range plasmids. Simultaneous usage of pBBR1- and RK2-based pLX vectors in a two-plasmid/one-Agrobacterium strain strategy allowed multigene delivery to plants. Adoption of pLX vectors will facilitate routine plant transformations and targeted mutagenesis, as well as complex part and circuit characterization. es_ES
dc.description.sponsorship We thank Victor de Lorenzo, Salome Prat, and Stephan Winter for supply of materials. We are grateful to Jose Luis Martinez for use of the Infinite M200 fluorometer, Beatriz Garcia for technical assistance and Catherine Mark for manuscript editing. This work was funded by grants BIO2013-49053-R, BIO2013-42193-R, BIO2016-80572-R and Plant KBBE PCIN-2013-056 from the Spanish Ministerio de Ciencia y Economia. es_ES
dc.language Inglés es_ES
dc.publisher American Chemical Society es_ES
dc.relation MINECO/BIO2013-49053-R es_ES
dc.relation MINECO/BIO2016-80572-R es_ES
dc.relation MINECO/KBBE PCIN-2013-056 es_ES
dc.relation MINECO/BIO2013-42193-R es_ES
dc.relation.ispartof ACS Synthetic Biology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Agrobacterium tumefaciens es_ES
dc.subject Binary T-DNA vector es_ES
dc.subject Ethanol-inducible switch es_ES
dc.subject Multigene transfer es_ES
dc.subject PBBR1 es_ES
dc.subject Plant biotechnology es_ES
dc.subject Potyviridae es_ES
dc.subject Ugandan cassava brown streak virus es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1021/acssynbio.6b00354 es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Pasin, F.; Bedoya, LC.; Bernabé-Orts, JM.; Gallo, A.; Simón-Mateo, C.; Orzáez Calatayud, DV.; García, JA. (2017). Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins. ACS Synthetic Biology. 6(10):1962-1968. https://doi.org/10.1021/acssynbio.6b00354 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1021/acssynbio.6b00354 es_ES
dc.description.upvformatpinicio 1962 es_ES
dc.description.upvformatpfin 1968 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 6 es_ES
dc.description.issue 10 es_ES
dc.identifier.eissn 2161-5063 es_ES
dc.identifier.pmid 28657330 es_ES
dc.relation.pasarela S\345863 es_ES
dc.contributor.funder MINECO es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


This item appears in the following Collection(s)

Show simple item record