Ressler, A.; Ródenas Rochina, J.; Ivankovic, M.; Ivankovic, H.; Rogina, A.; Ferrer, G. (2018). Injectable chitosan-hydroxyapatite hydrogels promote the osteogenic differentiation of mesenchymal stem cells. Carbohydrate Polymers. 197:469-477. https://doi.org/10.1016/j.carbpol.2018.06.029
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/148015
Title:
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Injectable chitosan-hydroxyapatite hydrogels promote the osteogenic differentiation of mesenchymal stem cells
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Author:
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Ressler, Antonia
Ródenas Rochina, Joaquín
Ivankovic, Marica
Ivankovic, Hrvoje
Rogina, Anamarija
Ferrer, G.G.
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UPV Unit:
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Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada
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Issued date:
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Abstract:
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[EN] Injectable hydrogels have emerged as promising biomaterials for tissue engineering applications. The goal of this study was to evaluate the potential of a pH-responsive chitosan-hydroxyapatite hydrogel to be used as ...[+]
[EN] Injectable hydrogels have emerged as promising biomaterials for tissue engineering applications. The goal of this study was to evaluate the potential of a pH-responsive chitosan-hydroxyapatite hydrogel to be used as a three-dimensional support for encapsulated mesenchymal stem cells (MSCs) osteogenic differentiation. In vitro enzymatic degradation of the hydrogel, during 28 days of incubation, in simulated physiological condiditons, was characterized by swelling measurements, molecular weight determination and SEM analysis of hydrogel microstructure. Osteogenic differentiation of encapsulated MSCs was confirmed by osteogenic Runx2, collagen type I and osteocalcin immunostaining and alkaline phosphatase quantification. The deposition of late osteogenic markers (calcium phosphates) detected by Alizarin red and von Kossa staining indicated an extracellular matrix mineralization.
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Subjects:
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Chitosan
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Differentiation
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Hydrogel
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Hydroxyapatite
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MSCs
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Osteogenesis
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Copyrigths:
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Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
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Source:
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Carbohydrate Polymers. (issn:
0144-8617
)
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DOI:
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10.1016/j.carbpol.2018.06.029
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Publisher:
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Elsevier
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Publisher version:
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https://doi.org/10.1016/j.carbpol.2018.06.029
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Project ID:
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info:eu-repo/grantAgreement/IP-2014-09-3752
MINECO/MAT2016-76039-C4-1-R
GENERALITAT VALENCIANA/APOSTD/2016/006
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Thanks:
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This work has been supported in part by the Croatian Science Foundation under the project IP-2014-09-3752 by the Spanish Ministry through the MAT2016-76039-C4-1-R project (including the FEDER financial support). Joaquin ...[+]
This work has been supported in part by the Croatian Science Foundation under the project IP-2014-09-3752 by the Spanish Ministry through the MAT2016-76039-C4-1-R project (including the FEDER financial support). Joaquin Rodenas-Rochina acknowledges funding by the Conselleria de Educacion, Investigacion, Cultura y Deporte (Generalitat Valenciana) and co-funding by Fondo Social Europeo (FSE) through APOSTD grant (APOSTD/2016/006).
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Type:
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Artículo
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