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Drought tolerance in Pinus halepensis seed sources as identified by distinctive physiological and molecular markers

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Drought tolerance in Pinus halepensis seed sources as identified by distinctive physiological and molecular markers

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dc.contributor.author Taïbi, Khaled es_ES
dc.contributor.author Campo García, Antonio Dámaso Del es_ES
dc.contributor.author Vilagrosa, Alberto es_ES
dc.contributor.author Belles Albert, José Mª es_ES
dc.contributor.author López-Gresa, María Pilar es_ES
dc.contributor.author Pla, Davinia es_ES
dc.contributor.author Calvete Chornet, Juan José es_ES
dc.contributor.author López-Nicolás, José M. es_ES
dc.contributor.author Mulet, José Miguel es_ES
dc.date.accessioned 2020-07-30T03:34:23Z
dc.date.available 2020-07-30T03:34:23Z
dc.date.issued 2017-07-24 es_ES
dc.identifier.uri http://hdl.handle.net/10251/148871
dc.description.abstract [EN] Drought is one of the main constraints determining forest species growth, survival and productivity, and therefore one of the main limitations for reforestation or afforestation. The aim of this study is to characterize the drought response at the physiological and molecular level of different Pinus halepensis (common name Aleppo pine) seed sources, previously characterized in field trials as drought-sensitive or drought-tolerant. This approach aims to identify different traits capable of predicting the ability of formerly uncharacterized seedlings to cope with drought stress. Gas-exchange, water potential, photosynthetic pigments, soluble sugars, free amino acids, glutathione and proteomic analyses were carried out on control and drought-stressed seedlings in greenhouse conditions. Gas-exchange determinations were also assessed in field-planted seedlings in order to validate the greenhouse experimental conditions. Drought-tolerant seed sources presented higher values of photosynthetic rates, water use efficiency, photosynthetic pigments and soluble carbohydrates concentrations. We observed the same pattern of variation of photosynthesis rate and maximal efficiency of PSII in field. Interestingly drought-tolerant seed sources exhibited increased levels of glutathione, methionine and cysteine. The proteomic profile of drought tolerant seedlings identified two heat shock proteins and an enzyme related to methionine biosynthesis that were not present in drought sensitive seedlings, pointing to the synthesis of sulfur amino acids as a limiting factor for drought tolerance in Pinus halepensis. Our results established physiological and molecular traits useful as distinctive markers to predict drought tolerance in Pinus halepensis provenances that could be reliably used in reforestation programs in drought prone areas. es_ES
dc.description.sponsorship This study is a part of the research project: "Application of molecular biology techniques in forest restoration in Mediterranean environments, PAID-05-11" funded by the Universitat Politecnica de Valencia (UPV), program for supporting R&D of new multidisciplinary research lines. The authors are grateful to the Ministerio de Economia y Competitividad AGL2014-57431-P. AV was supported by project Survive-2 (CGL2015-69773-C2-2-P MINECO/FEDER) by the Spanish Government and Prometeo program (DESESTRES-Generalitat Valenciana). CEAM is funded by Generalitat Valenciana. es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation.ispartof Frontiers in Plant Science es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Pinus halepensis es_ES
dc.subject Aleppo pine es_ES
dc.subject Drought tolerance es_ES
dc.subject Physiological response es_ES
dc.subject Soluble sugars es_ES
dc.subject Free amino acids es_ES
dc.subject Plant proteomics es_ES
dc.subject Glutathione es_ES
dc.subject.classification TECNOLOGIA DEL MEDIO AMBIENTE es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Drought tolerance in Pinus halepensis seed sources as identified by distinctive physiological and molecular markers es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fpls.2017.01202 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//PAID-05-11/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2014-57431-P/ES/PRODUCCION BIOLOGICA DE LOS PIGMENTOS ANTIOXIDANTES BETALAINAS Y EVALUACION DE SU CAPACIDAD FUNCIONAL EN MODELO IN VIVO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//CGL2015-69773-C2-2-P/ES/VULNERABILIDAD DE ESPECIES Y COMUNIDADES MEDITERRANEAS A LA RECURRENCIA DE INCENDIOS Y SEQUIAS EXTREMAS. EFECTOS SOBRE EL BALANCE HIDRICO Y LA DINAMICA DE LA VEGETACION./ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Hidráulica y Medio Ambiente - Departament d'Enginyeria Hidràulica i Medi Ambient es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Taïbi, K.; Campo García, ADD.; Vilagrosa, A.; Belles Albert, JM.; López-Gresa, MP.; Pla, D.; Calvete Chornet, JJ.... (2017). Drought tolerance in Pinus halepensis seed sources as identified by distinctive physiological and molecular markers. Frontiers in Plant Science. 8:1-13. https://doi.org/10.3389/fpls.2017.01202 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fpls.2017.01202 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 13 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.identifier.eissn 1664-462X es_ES
dc.identifier.pmid 28791030 es_ES
dc.identifier.pmcid PMC5523154 es_ES
dc.relation.pasarela S\341443 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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