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Lateral flow immunoassay for on-site detection of Xanthomonas arboricola pv. Pruni in symptomatic field samples

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Lateral flow immunoassay for on-site detection of Xanthomonas arboricola pv. Pruni in symptomatic field samples

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dc.contributor.author López-Soriano, P. es_ES
dc.contributor.author Noguera Murray, Patricia Silvestre es_ES
dc.contributor.author Gorris, María Teresa es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Maquieira Catala, Angel es_ES
dc.contributor.author Marco-Noales, E. es_ES
dc.contributor.author López, M.M. es_ES
dc.date.accessioned 2020-07-30T03:35:04Z
dc.date.available 2020-07-30T03:35:04Z
dc.date.issued 2017-04-27 es_ES
dc.identifier.issn 1932-6203 es_ES
dc.identifier.uri http://hdl.handle.net/10251/148892
dc.description.abstract [EN] Xanthomonas arboricola pv. pruni is a quarantine pathogen and the causal agent of the bacterial spot disease of stone fruits and almond, a major threat to Prunus species. Rapid and specific detection methods are essential to improve disease management, and therefore a prototype of a lateral flow immunoassay (LFIA) was designed for the detection of X. arboricola pv. pruni in symptomatic field samples. It was developed by producing polyclonal antibodies which were then combined with carbon nanoparticles and assembled on nitrocellulose strips. The specificity of the LFIA was tested against 87 X. arboricola pv. pruni strains from different countries worldwide, 47 strains of other Xanthomonas species and 14 strains representing other bacterial genera. All X. arboricola pv. pruni strains were detected and cross-reactions were observed only with four strains of X. arboricola pv. corylina, a hazelnut pathogen that does not share habitat with X. arboricola pv. pruni. The sensitivity of the LFIA was assessed with suspensions from pure cultures of three X. arboricola pv. pruni strains and with spiked leaf extracts prepared from four hosts inoculated with this pathogen (almond, apricot, Japanese plum and peach). The limit of detection observed with both pure cultures and spiked samples was 10(4) CFU ml(-1). To demonstrate the accuracy of the test, 205 samples naturally infected with X. arboricola pv. pruni and 113 samples collected from healthy plants of several different Prunus species were analyzed with the LFIA. Results were compared with those obtained by plate isolation and real time PCR and a high correlation was found among techniques. Therefore, we propose this LFIA as a screening tool that allows a rapid and reliable diagnosis of X. arboricola pv. pruni in symptomatic plants. es_ES
dc.description.sponsorship The work was supported by the following: Instituto Nacional de Tecnologia Agraria y Alimentaria, Project RTA-2011-00140-C03-01 (http://www.inia.es), PLS MTG EMN MML; Instituto Nacional de Tecnologia Agraria y Alimentaria, FPI-INIA grant (http://www.inia.es), PLS; Generalitat Valenciana (Prometeo II 2014/040) (http://www.gva.es), PN RP AM; Ministerio de Economia y Competitividad (MINECO) (CTQ2013-45875R) (http://www.mineco.gob.es), PN RP AM; European Social Fund, PLS MTG EMN MML; and European Regional Development Fund, PLS MTG EMN MML. es_ES
dc.language Inglés es_ES
dc.publisher Public Library of Science es_ES
dc.relation.ispartof PLoS ONE es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject PCR protocol es_ES
dc.subject Identification es_ES
dc.subject Device es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Lateral flow immunoassay for on-site detection of Xanthomonas arboricola pv. Pruni in symptomatic field samples es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1371/journal.pone.0176201 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//CTQ2013-45875-R/ES/BIOSENSADO EN SOPORTES INTERACTIVOS CON PROPIEDADES INTERFEROMETRICAS PARA APLICACIONES CLINICAS. DEMOSTRACION EN FARMACOGENETICA Y ALERGIAS MEDICAMENTOSAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//RTA2011-00140-C03-01/ES/Bases biológicas para la prevención de la enfermedad causada por Xanthomonas arboricola pv. pruni, patógeno de cuarentena de los frutales del género Prunas/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F040/ES/Estudio de estrategias fisico-químicas para el desarrollo de biosensores interferométricos en soportes interactivos de aplicación en clínica/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation López-Soriano, P.; Noguera Murray, PS.; Gorris, MT.; Puchades, R.; Maquieira Catala, A.; Marco-Noales, E.; López, M. (2017). Lateral flow immunoassay for on-site detection of Xanthomonas arboricola pv. Pruni in symptomatic field samples. PLoS ONE. 12(4):1-13. https://doi.org/10.1371/journal.pone.0176201 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1371/journal.pone.0176201 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 13 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 12 es_ES
dc.description.issue 4 es_ES
dc.identifier.pmid 28448536 es_ES
dc.identifier.pmcid PMC5407831 es_ES
dc.relation.pasarela S\337268 es_ES
dc.contributor.funder European Social Fund es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
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