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Time-sampled population sequencing reveals the interplay of selection and genetic drift in experimental evolution of Potato virus Y

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Time-sampled population sequencing reveals the interplay of selection and genetic drift in experimental evolution of Potato virus Y

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Nombre: Kutnjak;Elena;Ravnikar ...
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dc.contributor.author Kutnjak, D. es_ES
dc.contributor.author Elena Fito, Santiago Fco. es_ES
dc.contributor.author Ravnikar, M. es_ES
dc.date.accessioned 2020-07-31T03:31:37Z
dc.date.available 2020-07-31T03:31:37Z
dc.date.issued 2017-08 es_ES
dc.identifier.issn 0022-538X es_ES
dc.identifier.uri http://hdl.handle.net/10251/149086
dc.description.abstract [EN] RNA viruses are one of the fastest evolving biological entities. Within their hosts, they exist as genetically diverse populations (i.e., viral mutant swarms), which are sculpted by different evolutionary mechanisms, such as mutation, natural selection and genetic drift, and also the interactions between genetic variants within the mutant swarms. To elucidate the mechanisms that modulate the population diversity of an important plant pathogenic virus, we performed evolution experiments with Potato virus Y (PVY) in potato genotypes that differ in their defense response against the virus. Using deep sequencing of small RNAs, we followed the temporal dynamics of standing and newly-generated variation in the evolving viral lineages. A time-sampled approach allowed us to: (i) reconstruct theoretical haplotypes in the starting population by using clustering of single nucleotide polymorphisms' trajectories and (ii) use quantitative population genetics approaches to estimate the contribution of selection and genetic drift, and their interplay, to the evolution of the virus. We detected imprints of strong selective sweeps and narrow genetic bottlenecks, followed by the shift in frequency of selected haplotypes. Comparison of patterns of viral evolution in differently susceptible host genotypes indicated possible diversifying evolution of PVY in the less susceptible host (efficient in the accumulation of salicylicacid). es_ES
dc.description.sponsorship This study was supported by the Slovenian Research Agency (grants L4-5525 and P4-0165 and Ph.D. grant to D.K.). Work in Valencia was supported by Spain Ministry of Economy and Competitiveness (grant BFU2015-65037-P to S.F.E.), and short-term scientific mission support was provided to D.K. in the frame of EU-funded COST action FA1407. es_ES
dc.language Inglés es_ES
dc.publisher American Society for Microbiology es_ES
dc.relation.ispartof Journal of Virology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Potato virus Y es_ES
dc.subject Experimental evolution es_ES
dc.subject Genetic drift es_ES
dc.subject Natural selection es_ES
dc.subject SRNA deep sequencing es_ES
dc.title Time-sampled population sequencing reveals the interplay of selection and genetic drift in experimental evolution of Potato virus Y es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1128/JVI.00690-17 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ARRS//L4-5525/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/COST//FA1407/EU/Application of next generation sequencing for the study and diagnosis of plant viral diseases in agriculture/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ARRS//P4-0165/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2015-65037-P/ES/EVOLUCION DE VIRUS EN HUESPEDES CON SUSCEPTIBILIDAD VARIABLE: CONSECUENCIAS EN EFICACIA Y VIRULENCIA DE CAMBIOS EN LAS REDES INTERACTOMICAS DE PROTEINAS VIRUS-HUESPED/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Kutnjak, D.; Elena Fito, SF.; Ravnikar, M. (2017). Time-sampled population sequencing reveals the interplay of selection and genetic drift in experimental evolution of Potato virus Y. Journal of Virology. 91(16):1-17. https://doi.org/10.1128/JVI.00690-17 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1128/JVI.00690-17 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 17 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 91 es_ES
dc.description.issue 16 es_ES
dc.identifier.pmid 28592544 es_ES
dc.identifier.pmcid PMC5533922 es_ES
dc.relation.pasarela S\339123 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Slovenian Research Agency es_ES
dc.contributor.funder European Cooperation in Science and Technology es_ES
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