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Functionalization of PLLA with Polymer Brushes to Trigger the Assembly of Fibronectin into Nanonetworks

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Functionalization of PLLA with Polymer Brushes to Trigger the Assembly of Fibronectin into Nanonetworks

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dc.contributor.author Sprott, Mark Robert es_ES
dc.contributor.author Ferrer, GG es_ES
dc.contributor.author Dalby, Matthew J. es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.contributor.author Cantini, Marco es_ES
dc.date.accessioned 2020-10-05T07:00:18Z
dc.date.available 2020-10-05T07:00:18Z
dc.date.issued 2019-02-07 es_ES
dc.identifier.uri http://hdl.handle.net/10251/151103
dc.description.abstract [EN] Poly-l-lactic acid (PLLA) has been used as a biodegradable polymer for many years; the key characteristics of this polymer make it a versatile and useful resource for regenerative medicine. However, it is not inherently bioactive. Thus, here, a novel process is presented to functionalize PLLA surfaces with poly(ethyl acrylate) (PEA) brushes to provide biological functionality through PEA's ability to induce spontaneous organization of the extracellular matrix component fibronectin (FN) into physiological-like nanofibrils. This process allows control of surface biofunctionality while maintaining PLLA bulk properties (i.e., degradation profile, mechanical strength). The new approach is based on surface-initiated atomic transfer radical polymerization, which achieves a molecularly thin coating of PEA on top of the underlying PLLA. Beside surface characterization via atomic force microscopy, X-ray photoelectron spectroscopy and water contact angle to measure PEA grafting, the biological activity of this surface modification is investigated. PEA brushes trigger FN organization into nanofibrils, which retain their ability to enhance adhesion and differentiation of C2C12 cells. The results demonstrate the potential of this technology to engineer controlled microenvironments to tune cell fate via biologically active surface modification of an otherwise bioinert biodegradable polymer, gaining wide use in tissue engineering applications. es_ES
dc.description.sponsorship The authors acknowledge the EPSRC (EP/P001114/1) and MRC (MR/S005412/1) funding. The authors also acknowledge the EPSRC funding as part of the Doctoral Training Centre EP/F500424/1. This work was also funded by a grant from the UK Regenerative Medicine Platform. X-ray photoelectron spectroscopy was conducted by the National EPSRC XPS Users' Service (NEXUS), Newcastle. es_ES
dc.language Inglés es_ES
dc.publisher Wiley-VCH es_ES
dc.relation.ispartof Advanced Healthcare Materials (Online) es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Biomimetics es_ES
dc.subject Cell differentiation es_ES
dc.subject Fibronectin es_ES
dc.subject SI-ATRP es_ES
dc.subject Surface modification es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Functionalization of PLLA with Polymer Brushes to Trigger the Assembly of Fibronectin into Nanonetworks es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1002/adhm.201801469 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UKRI//EP%2FF500424%2F1/GB/LSI Doctoral Training Centres - Doctoral Training Centre in Cell & Proteomic Technologies/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UKRI//MR%2FS005412%2F1/GB/Engineered microenvironments to harvest stem cell response to viscosity for cartilage repair/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UKRI//EP%2FP001114%2F1/GB/Engineering growth factor microenvironments - a new therapeutic paradigm for regenerative medicine/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Sprott, MR.; Ferrer, G.; Dalby, MJ.; Salmerón Sánchez, M.; Cantini, M. (2019). Functionalization of PLLA with Polymer Brushes to Trigger the Assembly of Fibronectin into Nanonetworks. Advanced Healthcare Materials (Online). 8(3):1-12. https://doi.org/10.1002/adhm.201801469 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1002/adhm.201801469 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 12 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.description.issue 3 es_ES
dc.identifier.eissn 2192-2659 es_ES
dc.identifier.pmid 30609243 es_ES
dc.relation.pasarela S\410696 es_ES
dc.contributor.funder UK Regenerative Medicine Platform es_ES
dc.contributor.funder UK Research and Innovation es_ES
dc.contributor.funder Medical Research Council, Reino Unido es_ES
dc.contributor.funder Engineering and Physical Sciences Research Council, Reino Unido es_ES
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