Gamermann, D.; Montagud Aquino, A.; Aparicio, P.; Navarro-Peris, E.; Triana, J.; Villatoro, F.; Urchueguía Schölzel, JF.... (2012). A Modular Synthetic Device to Calibrate Promoters. Journal of Biological System. 20(1):37-55. https://doi.org/10.1142/S0218339012500015
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/152279
Title:
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A Modular Synthetic Device to Calibrate Promoters
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Author:
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Gamermann, Daniel
Montagud Aquino, Arnau
Aparicio, P.
Navarro-Peris, Emilio
Triana, J.
Villatoro, F.R.
Urchueguía Schölzel, Javier Fermín
Fernández de Córdoba, Pedro
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UPV Unit:
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Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada
Universitat Politècnica de València. Instituto Universitario de Matemática Pura y Aplicada - Institut Universitari de Matemàtica Pura i Aplicada
Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada
Universitat Politècnica de València. Departamento de Matemática Aplicada - Departament de Matemàtica Aplicada
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Issued date:
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Abstract:
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[EN] In this contribution, a design of a synthetic calibration genetic circuit to characterize the relative strength of different sensing promoters is proposed and its specifications and performance are analyzed via an ...[+]
[EN] In this contribution, a design of a synthetic calibration genetic circuit to characterize the relative strength of different sensing promoters is proposed and its specifications and performance are analyzed via an effective mathematical model. Our calibrator device possesses certain novel and useful features like modularity (and thus the possibility of being used in many different biological contexts), simplicity, being based on a single cell, high sensitivity and fast response. To uncover the critical model parameters and the corresponding parameter domain at which the calibrator performance will be optimal, a sensitivity analysis of the model parameters was carried out over a given range of sensing protein concentrations (acting as input). Our analysis suggests that the half saturation constants for repression, sensing and difference in binding cooperativity (Hill coefficients) for repression are the key to the performance of the proposed device. They furthermore are determinant for the sensing speed of the device, showing that it is possible to produce detectable differences in the repression protein concentrations and in turn in the corresponding fluorescence in less than two hours. This analysis paves the way for the design, experimental construction and validation of a new family of functional genetic circuits for the purpose of calibrating promoters.
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Subjects:
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Calibration
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Effective Modeling of Gene Circuits
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Gene Promoter
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Parameter Analysis
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Synthetic Biology
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Synthetic Genetic Circuits
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Copyrigths:
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Reserva de todos los derechos
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Source:
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Journal of Biological System. (issn:
0218-3390
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DOI:
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10.1142/S0218339012500015
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Publisher:
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WORLD SCIENTIFIC PUBL CO PTE LTD
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Publisher version:
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https://doi.org/10.1142/S0218339012500015
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Project ID:
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MICINN/TIN2009-12359
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Thanks:
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This work has been funded by MICINN TIN2009-12359 project ArtBioCom, the Spanish Ministerio de Educacion y Ciencia through the program Juan de la Cierva, the FPI grant program of the Generalitat Valenciana and the Beca de ...[+]
This work has been funded by MICINN TIN2009-12359 project ArtBioCom, the Spanish Ministerio de Educacion y Ciencia through the program Juan de la Cierva, the FPI grant program of the Generalitat Valenciana and the Beca de recerca predoctoral from the Universitat Rovira i Virgili. The authors would also like to thank the Valencia iGEM 2007 team and Enrique O'Connor and his group from the Centro de Investigacion Prncipe Felipe in the Universidad de Valencia for useful discussions.
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Type:
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Artículo
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