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DNA synthesis determines the binding mode of the human mitochondrial single-stranded DNA-binding protein

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DNA synthesis determines the binding mode of the human mitochondrial single-stranded DNA-binding protein

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Nombre: Morin;Cerrón;Jarillo ...
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dc.contributor.author Morin, J.A. es_ES
dc.contributor.author Cerrón, F. es_ES
dc.contributor.author Jarillo, J. es_ES
dc.contributor.author Beltran-Heredia, E. es_ES
dc.contributor.author Ciesielski, G.L. es_ES
dc.contributor.author Arias-Gonzalez, J. R. es_ES
dc.contributor.author Kaguni, L.S. es_ES
dc.contributor.author Cao, F.J. es_ES
dc.contributor.author Ibarra, B. es_ES
dc.date.accessioned 2020-10-28T04:33:12Z
dc.date.available 2020-10-28T04:33:12Z
dc.date.issued 2017-07-07 es_ES
dc.identifier.issn 0305-1048 es_ES
dc.identifier.uri http://hdl.handle.net/10251/153374
dc.description.abstract [EN] Single-stranded DNA-binding proteins (SSBs) play a key role in genome maintenance, binding and organizing single-stranded DNA (ssDNA) intermediates. Multimeric SSBs, such as the human mitochondrial SSB (HmtSSB), present multiple sites to interact with ssDNA, which has been shown in vitro to enable them to bind a variable number of single-stranded nucleotides depending on the salt and protein concentration. It has long been suggested that different binding modes might be used selectively for different functions. To study this possibility, we used optical tweezers to determine and compare the structure and energetics of long, individual HmtSSB¿DNA complexes assembled on preformed ssDNA and on ssDNA generated gradually during `in situ¿ DNA synthesis. We show that HmtSSB binds to preformed ss-DNA in two major modes, depending on salt and protein concentration. However, when protein binding was coupled to strand-displacement DNA synthesis, only one of the two binding modes was observed under all experimental conditions. Our results reveal a key role for the gradual generation of ssDNA in modulating the binding mode of a multimeric SSB protein and consequently, in generating the appropriate nucleoprotein structure for DNA synthetic reactions required for genome maintenance. es_ES
dc.description.sponsorship We are grateful to Prof. M. Salas laboratory (CBMSO-CSIC) for generously providing the Phi29 DNA polymerase and to Juan P. García Villaluenga (UCM) for useful discussions. Spanish Ministry of Economy and Competitiveness [MAT2015-71806-R to J.R.A-G, FIS2010-17440, FIS2015-67765-R to F.J.C., BFU2012-31825, BFU2015-63714-R to B.I.]; Spanish Ministry of Education, Culture and Sport [FPU13/02934 to J.J., FPU13/02826 to E.B-H.]; National Institutes of Health [GM45925 to L.S.K.]; University of Tampere (to G.L.C.); Programa de Financiacion Universidad Complutense de Madrid-Santander Universidades [CT45/15-CT46/15 to F.C.]. Funding for open access charge: Spanish Ministry of Economy and Competitiveness [BFU2015-63714-R]. es_ES
dc.language Inglés es_ES
dc.publisher Oxford University Press es_ES
dc.relation.ispartof Nucleic Acids Research es_ES
dc.rights Reconocimiento - No comercial (by-nc) es_ES
dc.subject DNA es_ES
dc.subject DNA polymerase es_ES
dc.subject Replication es_ES
dc.subject Single-molecule es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title DNA synthesis determines the binding mode of the human mitochondrial single-stranded DNA-binding protein es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/nar/gkx395 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//FIS2010-17440/ES/FISICA DE LOS PROCESOS FUERA DEL EQUILIBRIO: RETROALIMENTACION, ENTROPIA E INFORMACION/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIH//GM45925/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2012-31825/ES/DINAMICA DE LA REPLICACION DEL ADN MITOCONDRIAL A NIVEL DE MOLECULAS INDIVIDUALES./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2015-71806-R/ES/INFLUENCIA DEL CALOR EMITIDO POR NANOPARTICULAS MAGNETICAS SOBRE BIOMOLECULAS DETERMINADO MEDIANTE PINZAS OPTICAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//FIS2015-67765-R/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2015-63714-R/ES/CARACTERIZACION A NIVEL DE MOLECULAS INDIVIDUALES DE LA DINAMICA DEL FUNCIONAMIENTO COORDINADO DE LAS PROTEINAS IMPLICADAS EN LAS REPLICACION DEL ADN MITOCONDRIAL HUMANO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MECD//FPU13%2F02934/ES/FPU13%2F02934/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MECD//FPU13%2F02826/ES/FPU13%2F02826/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UCM//CT45%2F15-CT46%2F15/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.description.bibliographicCitation Morin, J.; Cerrón, F.; Jarillo, J.; Beltran-Heredia, E.; Ciesielski, G.; Arias-Gonzalez, JR.; Kaguni, L.... (2017). DNA synthesis determines the binding mode of the human mitochondrial single-stranded DNA-binding protein. Nucleic Acids Research. 45(12):7237-7248. https://doi.org/10.1093/nar/gkx395 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1093/nar/gkx395 es_ES
dc.description.upvformatpinicio 7237 es_ES
dc.description.upvformatpfin 7248 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 45 es_ES
dc.description.issue 12 es_ES
dc.identifier.pmid 28486639 es_ES
dc.identifier.pmcid PMC5499585 es_ES
dc.relation.pasarela S\407987 es_ES
dc.contributor.funder University of Tampere es_ES
dc.contributor.funder Santander Universidades es_ES
dc.contributor.funder Universidad Complutense de Madrid es_ES
dc.contributor.funder National Institutes of Health, EEUU es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Ministerio de Educación, Cultura y Deporte es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
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