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A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins

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A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins

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dc.contributor.author Julve Parreño, Jose Manuel es_ES
dc.contributor.author Huet, Estefania es_ES
dc.contributor.author Fernandez-del-Carmen, Asun es_ES
dc.contributor.author Segura, Alvaro es_ES
dc.contributor.author Venturi, Micol es_ES
dc.contributor.author Gandia, Antoni es_ES
dc.contributor.author Pan, Wei-song es_ES
dc.contributor.author Albaladejo, Irene es_ES
dc.contributor.author Forment Millet, José Javier es_ES
dc.contributor.author Pla, Davinia es_ES
dc.contributor.author Wigdorovitz, Andrés es_ES
dc.contributor.author Calvete, Juan J. es_ES
dc.contributor.author Gutierrez, Carlos es_ES
dc.contributor.author Gutiérrez, José María es_ES
dc.contributor.author GRANELL RICHART, ANTONIO es_ES
dc.contributor.author Orzáez Calatayud, Diego Vicente es_ES
dc.date.accessioned 2020-11-04T04:31:21Z
dc.date.available 2020-11-04T04:31:21Z
dc.date.issued 2018-03 es_ES
dc.identifier.issn 1467-7644 es_ES
dc.identifier.uri http://hdl.handle.net/10251/154014
dc.description.abstract [EN] Antivenoms developed from the plasma of hyperimmunized animals are the only effective treatment available against snakebite envenomation but shortage of supply contributes to the high morbidity and mortality toll of this tropical disease. We describe a synthetic biology approach to affordable and cost-effective antivenom production based on plant-made recombinant polyclonal antibodies (termed pluribodies). The strategy takes advantage of virus superinfection exclusion to induce the formation of somatic expression mosaics in agroinfiltrated plants, which enables the expression of complex antibody repertoires in a highly reproducible manner. Pluribodies developed using toxin-binding genetic information captured from peripheral blood lymphocytes of hyperimmunized camels recapitulated the overall binding activity of the immune response. Furthermore, an improved plant-made antivenom (plantivenom) was formulated using an invitro selected pluribody against Bothrops asper snake venom toxins and has been shown to neutralize a wide range of toxin activities and provide protection against lethal venom doses in mice. es_ES
dc.description.sponsorship We thank Brian J. Robinson for his careful revision of the manuscript. The work carried out at IBMCP-CSIC received financial support from MINECO and ERDF (project grants IPT-2011-0720-010000, BIO2013-42193-R and BIO2016-78601-R). Research at IBV-CSIC was supported by grant BFU2013-42833-P from the Spanish Ministry of Economy and Competitiveness, Madrid, Spain. The authors declare no conflict of interest es_ES
dc.language Inglés es_ES
dc.publisher Blackwell Publishing es_ES
dc.relation.ispartof Plant Biotechnology Journal es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Recombinant polyclonal antibodies es_ES
dc.subject Molecular pharming es_ES
dc.subject Snake antivenoms es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/pbi.12823 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//IPT-2011-0720-010000/ES/PRODUCCIÓN DE PROTEINAS TERAPEUTICAS EN BIOFACTORIAS VEGETALES. PROTEBIOV./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2013-42193-R/ES/GREEN SWITCHES: DISEÑO DE CIRCUITOS GENETICOS ARTIFICIALES PARA LA PRODUCCION DE PROTEINAS RECOMBINANTES Y EL ENRIQUECIMIENTO NUTRICIONAL DE PLANTAS SOLANACEAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2016-78601-R/ES/DISEÑO DE CIRCUITOS GENICOS SINTETICOS Y ORTOGONALES PARA PLANTAS MEDIANTE EL USO DE FACTORES PROGRAMABLES DE UNION A DNA BASADOS EN LA ARQUITECTURA CRISPR-CAS9./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2013-42833-P/ES/VENOMICA DE ULTIMA GENERACION/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Julve Parreño, JM.; Huet, E.; Fernandez-Del-Carmen, A.; Segura, A.; Venturi, M.; Gandia, A.; Pan, W.... (2018). A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins. Plant Biotechnology Journal. 16(3):727-736. https://doi.org/10.1111/pbi.12823 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1111/pbi.12823 es_ES
dc.description.upvformatpinicio 727 es_ES
dc.description.upvformatpfin 736 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 16 es_ES
dc.description.issue 3 es_ES
dc.identifier.pmid 28850773 es_ES
dc.identifier.pmcid PMC5814581 es_ES
dc.relation.pasarela S\356424 es_ES
dc.contributor.funder Ministerio de Ciencia, Innovación y Universidades es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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