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First RNA-seq approach to study fruit set and parthenocarpy in zucchini (Cucurbita pepo L.)

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First RNA-seq approach to study fruit set and parthenocarpy in zucchini (Cucurbita pepo L.)

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dc.contributor.author Pomares-Viciana, T es_ES
dc.contributor.author Del Rio-Celestino, M es_ES
dc.contributor.author Roman, B es_ES
dc.contributor.author Die, J es_ES
dc.contributor.author Picó Sirvent, María Belén es_ES
dc.contributor.author Gómez, P es_ES
dc.date.accessioned 2020-11-07T04:32:10Z
dc.date.available 2020-11-07T04:32:10Z
dc.date.issued 2019-02-06 es_ES
dc.identifier.issn 1471-2229 es_ES
dc.identifier.uri http://hdl.handle.net/10251/154378
dc.description.abstract [EN] Background: Zucchini fruit set can be limited due to unfavourable environmental conditions in off-seasons crops that caused ineffective pollination/fertilization. Parthenocarpy, the natural or artificial fruit development without fertilization, has been recognized as an important trait to avoid this problem, and is related to auxin signalling. Nevertheless, differences found in transcriptome analysis during early fruit development of zucchini suggest that other complementary pathways could regulate fruit formation in parthenocarpic cultivars of this species. The development of next-generation sequencing technologies (NGS) as RNA-sequencing (RNA-seq) opens a new horizon for mapping and quantifying transcriptome to understand the molecular basis of pathways that could regulate parthenocarpy in this species. The aim of the current study was to analyze fruit transcriptome of two cultivars of zucchini, a non-parthenocarpic cultivar and a parthenocarpic cultivar, in an attempt to identify key genes involved in parthenocarpy. Results: RNA-seq analysis of six libraries (unpollinated, pollinated and auxin treated fruit in a non-parthenocarpic and parthenocarpic cultivar) was performed mapping to a new version of C. pepo transcriptome, with a mean of 92% success rate of mapping. In the non-parthenocarpic cultivar, 6479 and 2186 genes were differentially expressed (DEGs) in pollinated fruit and auxin treated fruit, respectively. In the parthenocarpic cultivar, 10,497 in pollinated fruit and 5718 in auxin treated fruit. A comparison between transcriptome of the unpollinated fruit for each cultivar has been performed determining that 6120 genes were differentially expressed. Annotation analysis of these DEGs revealed that cell cycle, regulation of transcription, carbohydrate metabolism and coordination between auxin, ethylene and gibberellin were enriched biological processes during pollinated and parthenocarpic fruit set. Conclusion: This analysis revealed the important role of hormones during fruit set, establishing the activating role of auxins and gibberellins against the inhibitory role of ethylene and different candidate genes that could be useful as markers for parthenocarpic selection in the current breeding programs of zucchini. es_ES
dc.description.sponsorship Research worked is supported by the project RTA2014-00078 from the Spanish Institute of Agronomy Research INIA (Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria) and also PP.AVA.AVA201601.7, FEDER y FSE (Programa Operativo FSE de Andalucia 2007-2013 "Andalucia se mueve con Europa"). TPV is supported by a FPI scholarship from RTA2011-00044-C02-01/02 project of INIA. The funding agencies were not involved in the design of the study, collection, analysis, and interpretation of data and in writing the manuscript. es_ES
dc.language Inglés es_ES
dc.publisher Springer (Biomed Central Ltd.) es_ES
dc.relation.ispartof BMC Plant Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Cucurbita pepo es_ES
dc.subject Zucchini es_ES
dc.subject Parthenocarpy es_ES
dc.subject Fruit set es_ES
dc.subject Differential gene expression es_ES
dc.subject RNA-seq es_ES
dc.subject.classification GENETICA es_ES
dc.title First RNA-seq approach to study fruit set and parthenocarpy in zucchini (Cucurbita pepo L.) es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/s12870-019-1632-2 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//RTA2011-00044-C02-01/ES/Desarrollo competitivo de nuevos cultivares de calabacín y ampliación de la plataforma genómica para la mejora de esta hortaliza/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//RTA2014-00078-00-00/ES/Mejora de la calidad y resistencia de calabacín: aproximación genómica/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Junta de Andalucía//PP.AVA.AVA201601.7/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//RTA2011-00044-C02-02/ES/Desarrollo competitivo de nuevos cultivares de calabacín y ampliación de la plataforma genómica para la mejora de esta hortaliza/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Pomares-Viciana, T.; Del Rio-Celestino, M.; Roman, B.; Die, J.; Picó Sirvent, MB.; Gómez, P. (2019). First RNA-seq approach to study fruit set and parthenocarpy in zucchini (Cucurbita pepo L.). BMC Plant Biology. 19:1-20. https://doi.org/10.1186/s12870-019-1632-2 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1186/s12870-019-1632-2 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 20 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 19 es_ES
dc.identifier.pmid 30727959 es_ES
dc.identifier.pmcid PMC6366093 es_ES
dc.relation.pasarela S\383955 es_ES
dc.contributor.funder Junta de Andalucía es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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dc.subject.ods 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible es_ES


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