Pascual-Ahuir Giner, MD.; González-Cantó, E.; Juyoux, P.; Pable, J.; Poveda-Huertes, D.; Saiz-Balbastre Sandra; Squeo, S.... (2019). Dose dependent gene expression is dynamically modulated by the history, physiology and age of yeast cells. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. 1862(4):457-471. https://doi.org/10.1016/j.bbagrm.2019.02.009
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/154396
Title:
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Dose dependent gene expression is dynamically modulated by the history, physiology and age of yeast cells
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Author:
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Pascual-Ahuir Giner, María Desamparados
González-Cantó, Eva
Juyoux, Pauline
Pable, Julia
Poveda-Huertes, Daniel
Saiz-Balbastre Sandra
Squeo, Sonia
Ureña-Marco, Alvaro
Vanacloig-Pedrós, Elena
Zaragoza-Infante, Laura
Proft, Markus Hans
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UPV Unit:
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Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
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Issued date:
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Abstract:
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[EN] Cells respond to external stimuli with transient gene expression changes in order to adapt to environmental alterations. However, the dose response profile of gene
induction upon a given stress depends on many intrinsic ...[+]
[EN] Cells respond to external stimuli with transient gene expression changes in order to adapt to environmental alterations. However, the dose response profile of gene
induction upon a given stress depends on many intrinsic and extrinsic factors. Here we show that the accurate quantification of dose dependent gene expression by
live cell luciferase reporters reveals fundamental insights into stress signaling. We make the following discoveries applying this non-invasive reporter technology. (1)
Signal transduction sensitivities can be compared and we apply this here to salt, oxidative and xenobiotic stress responsive transcription factors. (2) Stress signaling
depends on where and how the damage is generated within the cell. Specifically we show that two ROS-generating agents, menadione and hydrogen peroxide, differ
in their dependence on mitochondrial respiration. (3) Stress signaling is conditioned by the cells history. We demonstrate here that positive memory or an acquired
resistance towards oxidative stress is induced dependent on the nature of the previous stress experience. (4) The metabolic state of the cell impinges on the sensitivity
of stress signaling. This is shown here for the shift towards higher stress doses of the response profile for yeast cells moved from complex to synthetic medium. (5) The
age of the cell conditions its transcriptional response capacity, which is demonstrated by the changes of the dose response to oxidative stress during both replicative
and chronological aging. We conclude that capturing dose dependent gene expression in real time will be of invaluable help to understand stress signaling and its
dynamic modulation.
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Copyrigths:
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Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
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Source:
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Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. (issn:
1874-9399
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DOI:
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10.1016/j.bbagrm.2019.02.009
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Publisher:
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Elsevier
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Publisher version:
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https://doi.org/10.1016/j.bbagrm.2019.02.009
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Project ID:
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info:eu-repo/grantAgreement/MINECO//BFU2016-75792-R/ES/ADAPTACION COORDINADA A ESTRES MEDIANTE LA MODULACION DE LA HOMEOSTASIS MITOCONDRIAL Y LA ACTIVACION SELECTIVA DEL TRANSPORTE MULTI-DROGA/
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Thanks:
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The authors thank Daniel E. Gottschling (Fred Hutchinson Cancer Research Center, Seattle, US) for the kind gift of MEP yeast strain UCC4925. This work was supported by the Ministerio de Economia y Competitividad (grant ...[+]
The authors thank Daniel E. Gottschling (Fred Hutchinson Cancer Research Center, Seattle, US) for the kind gift of MEP yeast strain UCC4925. This work was supported by the Ministerio de Economia y Competitividad (grant number BFU2016-75792-R).
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Type:
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Artículo
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