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Dose dependent gene expression is dynamically modulated by the history, physiology and age of yeast cells

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Dose dependent gene expression is dynamically modulated by the history, physiology and age of yeast cells

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dc.contributor.author Pascual-Ahuir Giner, María Desamparados es_ES
dc.contributor.author González-Cantó, Eva es_ES
dc.contributor.author Juyoux, Pauline es_ES
dc.contributor.author Pable, Julia es_ES
dc.contributor.author Poveda-Huertes, Daniel es_ES
dc.contributor.author Saiz-Balbastre Sandra es_ES
dc.contributor.author Squeo, Sonia es_ES
dc.contributor.author Ureña-Marco, Alvaro es_ES
dc.contributor.author Vanacloig-Pedrós, Elena es_ES
dc.contributor.author Zaragoza-Infante, Laura es_ES
dc.contributor.author Proft, Markus Hans es_ES
dc.date.accessioned 2020-11-07T04:32:56Z
dc.date.available 2020-11-07T04:32:56Z
dc.date.issued 2019-04 es_ES
dc.identifier.issn 1874-9399 es_ES
dc.identifier.uri http://hdl.handle.net/10251/154396
dc.description.abstract [EN] Cells respond to external stimuli with transient gene expression changes in order to adapt to environmental alterations. However, the dose response profile of gene induction upon a given stress depends on many intrinsic and extrinsic factors. Here we show that the accurate quantification of dose dependent gene expression by live cell luciferase reporters reveals fundamental insights into stress signaling. We make the following discoveries applying this non-invasive reporter technology. (1) Signal transduction sensitivities can be compared and we apply this here to salt, oxidative and xenobiotic stress responsive transcription factors. (2) Stress signaling depends on where and how the damage is generated within the cell. Specifically we show that two ROS-generating agents, menadione and hydrogen peroxide, differ in their dependence on mitochondrial respiration. (3) Stress signaling is conditioned by the cells history. We demonstrate here that positive memory or an acquired resistance towards oxidative stress is induced dependent on the nature of the previous stress experience. (4) The metabolic state of the cell impinges on the sensitivity of stress signaling. This is shown here for the shift towards higher stress doses of the response profile for yeast cells moved from complex to synthetic medium. (5) The age of the cell conditions its transcriptional response capacity, which is demonstrated by the changes of the dose response to oxidative stress during both replicative and chronological aging. We conclude that capturing dose dependent gene expression in real time will be of invaluable help to understand stress signaling and its dynamic modulation. es_ES
dc.description.sponsorship The authors thank Daniel E. Gottschling (Fred Hutchinson Cancer Research Center, Seattle, US) for the kind gift of MEP yeast strain UCC4925. This work was supported by the Ministerio de Economia y Competitividad (grant number BFU2016-75792-R). es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Dose dependent gene expression is dynamically modulated by the history, physiology and age of yeast cells es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.bbagrm.2019.02.009 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2016-75792-R/ES/ADAPTACION COORDINADA A ESTRES MEDIANTE LA MODULACION DE LA HOMEOSTASIS MITOCONDRIAL Y LA ACTIVACION SELECTIVA DEL TRANSPORTE MULTI-DROGA/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Pascual-Ahuir Giner, MD.; González-Cantó, E.; Juyoux, P.; Pable, J.; Poveda-Huertes, D.; Saiz-Balbastre Sandra; Squeo, S.... (2019). Dose dependent gene expression is dynamically modulated by the history, physiology and age of yeast cells. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. 1862(4):457-471. https://doi.org/10.1016/j.bbagrm.2019.02.009 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.bbagrm.2019.02.009 es_ES
dc.description.upvformatpinicio 457 es_ES
dc.description.upvformatpfin 471 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 1862 es_ES
dc.description.issue 4 es_ES
dc.identifier.pmid 30836134 es_ES
dc.relation.pasarela S\402176 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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