Locascio, AAM.; Marques Romero, MC.; García-Martínez, G.; Corratgé-Faillie, C.; Andrés-Colás, N.; Rubio, L.; Fernández, JA.... (2019). BCL2-ASSOCIATED ATHANOGENE4 Regulates the KAT1 Potassium Channel and Controls Stomatal Movement. PLANT PHYSIOLOGY. 181(3):1277-1294. https://doi.org/10.1104/pp.19.00224
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/156426
Título:
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BCL2-ASSOCIATED ATHANOGENE4 Regulates the KAT1 Potassium Channel and Controls Stomatal Movement
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Autor:
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Locascio, Antonella Anna Maria
Marques Romero, Mª Carmen
García-Martínez, Guillermo
Corratgé-Faillie, Claire
Andrés-Colás, Nuria
Rubio, Lourdes
Fernández, José Antonio
Véry, Anne-Aliénor
Mulet, José Miguel
Yenush, Lynne
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Entidad UPV:
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Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
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Fecha difusión:
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Resumen:
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[EN] Potassium (K+) is a key monovalent cation necessary for multiple aspects of cell growth and survival. In plants, this cation also plays a key role in the control of stomatal movement. KAT1 and its homolog KAT2 are the ...[+]
[EN] Potassium (K+) is a key monovalent cation necessary for multiple aspects of cell growth and survival. In plants, this cation also plays a key role in the control of stomatal movement. KAT1 and its homolog KAT2 are the main inward rectifying channels present in guard cells, mediating K+ influx into these cells, resulting in stomatal opening. To gain further insight into the regulation of these channels, we performed a split-ubiquitin protein-protein interaction screen searching for KAT1 interactors in Arabidopsis (Arabidopsis thaliana). We characterized one of these candidates, BCL2-ASSOCIATED ATHANOGENE4 (BAG4), in detail using biochemical and genetic approaches to confirm this interaction and its effect on KAT1 activity. We show that BAG4 improves KAT1-mediated K+ transport in two heterologous systems and provide evidence that in plants, BAG4 interacts with KAT1 and favors the arrival of KAT1 at the plasma membrane. Importantly, lines lacking or overexpressing the BAG4 gene show altered KAT1 plasma membrane accumulation and alterations in stomatal movement. Our data allowed us to identify a KAT1 regulator and define a potential target for the plant BAG family. The identification of physiologically relevant regulators of K+ channels will aid in the design of approaches that may impact drought tolerance and pathogen susceptibility.
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Palabras clave:
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Plant K+ channel
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Endoplasmic-Reticulum
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BCL-2-Associated athanogene
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Saccharomyces-Cerevisiae
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Plasma-Membrane
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Abscisic-Acid
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Arabidopsis
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Expression
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Family
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Trafficking
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Derechos de uso:
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Reserva de todos los derechos
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Fuente:
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PLANT PHYSIOLOGY. (issn:
0032-0889
)
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DOI:
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10.1104/pp.19.00224
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Editorial:
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American Society of Plant Biologists
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Versión del editor:
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https://doi.org/10.1104/pp.19.00224
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Código del Proyecto:
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info:eu-repo/grantAgreement/MINECO//BIO2016-77776-P/ES/DESCIFRANDO LA REGULACION DE TRANSPORTADORES DE POTASIO EN PLANTAS Y LEVADURAS/
info:eu-repo/grantAgreement/MINECO//BIO2016-81957-REDT/ES/SISTEMAS DE TRANSPORTE DE SODIO Y POTASIO EN PLANTAS/
info:eu-repo/grantAgreement/GVA//AICO%2F2018%2F300/
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Agradecimientos:
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The authors would like to thank Elena Moreno, María José Falaguer, Alejandro Mossi, Sara Aljama, José Antonio Navarro, Vicente Pallás, Daniel Franco-Aragón, Jorge Lozano, and Stan Gelvin for assistance in the completion ...[+]
The authors would like to thank Elena Moreno, María José Falaguer, Alejandro Mossi, Sara Aljama, José Antonio Navarro, Vicente Pallás, Daniel Franco-Aragón, Jorge Lozano, and Stan Gelvin for assistance in the completion of this work and for providing reagents. This work was supported by the Spanish Ministry of Economy and Competitiveness (BIO201677776-P and BIO2016-81957-REDT) and the Valencian Government (AICO/2018/300).
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Tipo:
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Artículo
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