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Proteomic analysis of calcium-enriched sol-gel biomaterials

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Proteomic analysis of calcium-enriched sol-gel biomaterials

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dc.contributor.author Romero-Gavilán, F. es_ES
dc.contributor.author Araújo-Gomes, Nuno es_ES
dc.contributor.author Cerqueira, A. es_ES
dc.contributor.author García-Arnáez, I. es_ES
dc.contributor.author Martínez-Ramos, Cristina es_ES
dc.contributor.author Azkargorta, M. es_ES
dc.contributor.author Iloro, I. es_ES
dc.contributor.author Elortza, F. es_ES
dc.contributor.author Gurruchaga, M. es_ES
dc.contributor.author Suay, J. es_ES
dc.contributor.author Goñi, I. es_ES
dc.date.accessioned 2021-01-12T21:02:38Z
dc.date.available 2021-01-12T21:02:38Z
dc.date.issued 2019-06 es_ES
dc.identifier.issn 0949-8257 es_ES
dc.identifier.uri http://hdl.handle.net/10251/158840
dc.description.abstract [EN] Calcium is an element widely used in the development of biomaterials for bone tissue engineering as it plays important roles in bone metabolism and blood coagulation. The Ca ions can condition the microenvironment at the tissue-material interface, affecting the protein deposition process and cell responses. The aim of this study was to analyze the changes in the patterns of protein adsorption on the silica hybrid biomaterials supplemented with different amounts of CaCl2, which can function as release vehicles. This characterization was carried out by incubating the Ca-biomaterials with human serum. LC-MS/MS analysis was used to characterize the adsorbed protein layers and compile a list of proteins whose affinity for the surfaces might depend on the CaCl2 content. The attachment of pro- and anti-clotting proteins, such as THRB, ANT3, and PROC, increased significantly on the Ca-materials. Similarly, VTNC and APOE, proteins directly involved on osteogenic processes, attached preferentially to these surfaces. To assess correlations with the proteomic data, these formulations were tested in vitro regarding their osteogenic and inflammatory potential, employing MC3T3-E1 and RAW 264.7 cell lines, respectively. The results confirmed a Ca dose-dependent osteogenic and inflammatory behavior of the materials employed, in accordance with the protein attachment patterns. es_ES
dc.description.sponsorship This work was supported by MINECO [MAT2017-86043-R]; Universitat Jaume I [Grant numbers Predoc/2014/25, UJI-B2017-37]; Basque Government [Grant numbers IT611-13, Predoc/2016/1/0141]; University of the Basque Country [Grant number UFI11/56]. CIC bioGUNE is supported by Basque Department of Industry, Tourism and Trade (Etortek and Elkartek programs), the Innovation Technology Department of the Bizkaia County; The ProteoRed-ISCIII (Grant PRB3 IPT17/0019); CIBERehd Network, and Severo Ochoa Grant (SEV-2016-0644). Authors would like to thank Antonio Coso and Jaime Franco (GMI-Ilerimplant) for their inestimable contribution to this study, and Raquel Oliver, Jose Ortega (UJI) and Iraide Escobes (CIC bioGUNE) for their valuable technical assistance. es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof JBIC Journal of Biological Inorganic Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Prothrombin es_ES
dc.subject Apolipoprotein E es_ES
dc.subject Blood clotting es_ES
dc.subject Vitronectin es_ES
dc.subject Bone regeneration es_ES
dc.subject.classification CIENCIA DE LOS MATERIALES E INGENIERIA METALURGICA es_ES
dc.title Proteomic analysis of calcium-enriched sol-gel biomaterials es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00775-019-01662-5 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2017-86043-R/ES/DESARROLLO DE IMPLANTES DENTALES CON PROPIEDADES OSTEOGENICAS PARA LA UNIVERSALIZACION DE RECEPTORES. DETERMINACION DE PATRONES DE PROTEINAS DE LA EFICACIA REGENERATIVA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UJI//Predoc%2F2014%2F25/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UJI//UJI-B2017-37/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Eusko Jaurlaritza//IT611-13/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Eusko Jaurlaritza//Predoc%2F2016%2F1%2F0141/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV%2FEHU//UFI11%2F56/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//SEV-2016-0644/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ISCIII//PRB3 IPT17%2F0019/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Romero-Gavilán, F.; Araújo-Gomes, N.; Cerqueira, A.; García-Arnáez, I.; Martínez-Ramos, C.; Azkargorta, M.; Iloro, I.... (2019). Proteomic analysis of calcium-enriched sol-gel biomaterials. JBIC Journal of Biological Inorganic Chemistry. 24(4):563-574. https://doi.org/10.1007/s00775-019-01662-5 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s00775-019-01662-5 es_ES
dc.description.upvformatpinicio 563 es_ES
dc.description.upvformatpfin 574 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 24 es_ES
dc.description.issue 4 es_ES
dc.relation.pasarela S\399800 es_ES
dc.contributor.funder Universitat Jaume I es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
dc.contributor.funder Gobierno Vasco/Eusko Jaurlaritza es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Universidad del País Vasco/Euskal Herriko Unibertsitatea es_ES
dc.contributor.funder Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas es_ES
dc.contributor.funder Agencia Estatal de Investigación
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