Añón, E.; Costero, AM.; Amorós Del Toro, P.; El Haskouri, J.; Martínez-Máñez, R.; Parra Álvarez, M.; Gil Grau, S.... (2020). Peptide-Capped Mesoporous Nanoparticles: Toward a more Efficient Internalization of Alendronate. ChemistrySelect. 5(12):3618-3625. https://doi.org/10.1002/slct.202000417
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/158947
Title:
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Peptide-Capped Mesoporous Nanoparticles: Toward a more Efficient Internalization of Alendronate
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Author:
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Añón, Elena
Costero, Ana M.
Amorós del Toro, Pedro
El Haskouri, Jamal
Martínez-Máñez, Ramón
Parra Álvarez, Margarita
Gil Grau, Salvador
Gaviña, Pablo
Terencio Silvestre, María Carmen
Alfonso-Navarro, María
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UPV Unit:
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Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic
Universitat Politècnica de València. Departamento de Química - Departament de Química
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Issued date:
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Abstract:
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[EN] Osteoporosis is an illness which appears when the osteoblast/osteoclast activities are unbalanced taking place bone resorption (caused by osteoclasts) in higher extension than bone formation (induced by osteoblasts). ...[+]
[EN] Osteoporosis is an illness which appears when the osteoblast/osteoclast activities are unbalanced taking place bone resorption (caused by osteoclasts) in higher extension than bone formation (induced by osteoblasts). Alendronate is one of the most used drugs for osteoporosis treatment despite its scarce bioavailability. Here we present the synthesis and characterization of mesoporous gated nanoparticles (two sets) for the controlled release of alendronate. The first set of nanoparticles (S1) were loaded with sulforhodamine B and capped with a peptide that could be selectively hydrolyzed by cathepsin K enzyme (overexpressed in osteoclasts). The second set (S2) was functionalized with aminopropyl moieties, loaded with nitrobenzofurazan labelled alendronate and capped with the same peptide. Both nanoparticles were internalized by RAW 264.7 macrophages (which could differentiate in osteoclasts) and were able to release its entrapped cargo in the presence of cathepsin K added in the macrophage lysates. Using S2 nanoparticles 4.2% of the total alendronate amount in contact with the cells is liberated inside them and could produce its therapeutic effect.
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Subjects:
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Alendronate
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Enzymes
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Cathepsin K
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Nanoparticles
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Osteoporosis
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Copyrigths:
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Cerrado |
Source:
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ChemistrySelect. (eissn:
2365-6549
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DOI:
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10.1002/slct.202000417
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Publisher:
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John Wiley & Sons
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Publisher version:
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https://doi.org/10.1002/slct.202000417
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Project ID:
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MICINN/RTI2018-100910-B-C42
GENERALITAT VALENCIANA/PROMETEO/2018/024
AEI/RTI2018-100910-B-C41-AR
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Thanks:
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We thank the Spanish Government (RTI2018-100910-B-C41, RTI2018-100910-B-C42 (MCUI/AEI/FEDER, UE)) and the Generalitat Valenciana (PROMETEU/2018/024) for support. SCSIE (Universitat de Valencia) is gratefully acknowledged ...[+]
We thank the Spanish Government (RTI2018-100910-B-C41, RTI2018-100910-B-C42 (MCUI/AEI/FEDER, UE)) and the Generalitat Valenciana (PROMETEU/2018/024) for support. SCSIE (Universitat de Valencia) is gratefully acknowledged for all the equipment employed. NMR was registered at the U26 facility of ICTS "NANBIOSIS" at the Universitat of Valencia.
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Type:
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Artículo
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