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Highly efficient construction of infectious viroid-derived clones

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Highly efficient construction of infectious viroid-derived clones

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Márquez-Molins, J.; Navarro Bohigues, JA.; Pallás Benet, V.; Gomez, GG. (2019). Highly efficient construction of infectious viroid-derived clones. Plant Methods. 15:1-8. https://doi.org/10.1186/s13007-019-0470-4

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Title: Highly efficient construction of infectious viroid-derived clones
Author: Márquez-Molins, Joan NAVARRO BOHIGUES, JOSE ANTONIO Pallás Benet, Vicente Gomez, Gustavo Germán
UPV Unit: Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
Issued date:
Abstract:
[EN] Background Viroid research generally relies on infectious cDNA clones that consist of dimers of the entire viroid sequence. At present, those dimers are generated by self-ligation of monomeric cDNA, a strategy that ...[+]
Subjects: Viroid , Cloning , Dimers , IIs enzymes , Agro-infiltration
Copyrigths: Reconocimiento (by)
Source:
Plant Methods. (issn: 1746-4811 )
DOI: 10.1186/s13007-019-0470-4
Publisher:
Springer (Biomed Central Ltd.)
Publisher version: https://doi.org/10.1186/s13007-019-0470-4
Project ID:
MINECO PROYECTOS DE I+D+I, Programa Estatal Investigación, Desarrollo e Innovacion Orientada a retos de la Sociedad /AGL2016-79825-R
MINECO/BIO2017-88321-R
Thanks:
This work was supported by the Spanish Ministry of Economy and Competitiveness (co-supported by FEDER) Grants BIO2017-88321-R (VP) and AGL2016-79825-R (GG). The funders had no role in the experiment design, data analysis, ...[+]
Type: Artículo

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