- -

A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by


  • Estadisticas de Uso

A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC

Show full item record

Kenesi, E.; Carbonell, A.; Lozsa, R.; Vertessy, B.; Lakatos, L. (2017). A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC. Nucleic Acids Research. 45(13):7736-7750. https://doi.org/10.1093/nar/gkx379

Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/161199

Files in this item

Item Metadata

Title: A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC
Author: Kenesi, Erzsebet CARBONELL, ALBERTO Lozsa, Rita Vertessy, Beata Lakatos, Lorant
UPV Unit: Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
Issued date:
[EN] In most eukaryotes, RNA silencing is an adaptive immune system regulating key biological processes including antiviral defense. To evade this response, viruses of plants, worms and insects have evolved viral suppressors ...[+]
Subjects: RNA silencing , Plant virus, siRNA , AGO , Immunoprecipitation , Silencing suppressor
Copyrigths: Reconocimiento (by)
Nucleic Acids Research. (issn: 0305-1048 )
DOI: 10.1093/nar/gkx379
Oxford University Press
Publisher version: https://doi.org/10.1093/nar/gkx379
Project ID:
info:eu-repo/grantAgreement/EC/H2020/655841/EU/Genome-wide analysis of RNA and protein interacting profiles during a plant virus infection/
Hungarian Scientific Research Fund (OTKA) [K91042, NN107787, NN11024 to L.L.]; European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska Curie [655841 to A.C.]. Funding for open access ...[+]
Type: Artículo


Sayed, D., & Abdellatif, M. (2011). MicroRNAs in Development and Disease. Physiological Reviews, 91(3), 827-887. doi:10.1152/physrev.00006.2010

Martin, R. C., Liu, P.-P., Goloviznina, N. A., & Nonogaki, H. (2010). microRNA, seeds, and Darwin?: diverse function of miRNA in seed biology and plant responses to stress. Journal of Experimental Botany, 61(9), 2229-2234. doi:10.1093/jxb/erq063

BAULCOMBE, D. (2004). Crystal structure of p19 ? a universal suppressor of RNA silencing. Trends in Biochemical Sciences, 29(6), 279-281. doi:10.1016/j.tibs.2004.04.007 [+]
Sayed, D., & Abdellatif, M. (2011). MicroRNAs in Development and Disease. Physiological Reviews, 91(3), 827-887. doi:10.1152/physrev.00006.2010

Martin, R. C., Liu, P.-P., Goloviznina, N. A., & Nonogaki, H. (2010). microRNA, seeds, and Darwin?: diverse function of miRNA in seed biology and plant responses to stress. Journal of Experimental Botany, 61(9), 2229-2234. doi:10.1093/jxb/erq063

BAULCOMBE, D. (2004). Crystal structure of p19 ? a universal suppressor of RNA silencing. Trends in Biochemical Sciences, 29(6), 279-281. doi:10.1016/j.tibs.2004.04.007

Matzke, M. A., & Mosher, R. A. (2014). RNA-directed DNA methylation: an epigenetic pathway of increasing complexity. Nature Reviews Genetics, 15(6), 394-408. doi:10.1038/nrg3683

Bernstein, E., Caudy, A. A., Hammond, S. M., & Hannon, G. J. (2001). Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature, 409(6818), 363-366. doi:10.1038/35053110

Ender, C., & Meister, G. (2010). Argonaute proteins at a glance. Journal of Cell Science, 123(11), 1819-1823. doi:10.1242/jcs.055210

Hutvágner, G., Simard, M. J., Mello, C. C., & Zamore, P. D. (2004). Sequence-Specific Inhibition of Small RNA Function. PLoS Biology, 2(4), e98. doi:10.1371/journal.pbio.0020098

Meister, G. (2013). Argonaute proteins: functional insights and emerging roles. Nature Reviews Genetics, 14(7), 447-459. doi:10.1038/nrg3462

Wilkins, C., Dishongh, R., Moore, S. C., Whitt, M. A., Chow, M., & Machaca, K. (2005). RNA interference is an antiviral defence mechanism in Caenorhabditis elegans. Nature, 436(7053), 1044-1047. doi:10.1038/nature03957

Li, S., Liu, L., Zhuang, X., Yu, Y., Liu, X., Cui, X., … Chen, X. (2013). MicroRNAs Inhibit the Translation of Target mRNAs on the Endoplasmic Reticulum in Arabidopsis. Cell, 153(3), 562-574. doi:10.1016/j.cell.2013.04.005

Van Rij, R. P., Saleh, M.-C., Berry, B., Foo, C., Houk, A., Antoniewski, C., & Andino, R. (2006). The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster. Genes & Development, 20(21), 2985-2995. doi:10.1101/gad.1482006

Baulcombe, D. (2002). Viral suppression of systemic silencing. Trends in Microbiology, 10(7), 306-308. doi:10.1016/s0966-842x(02)02387-9

Maillard, P. V., Ciaudo, C., Marchais, A., Li, Y., Jay, F., Ding, S. W., & Voinnet, O. (2013). Antiviral RNA Interference in Mammalian Cells. Science, 342(6155), 235-238. doi:10.1126/science.1241930

Bronkhorst, A. W., & van Rij, R. P. (2014). The long and short of antiviral defense: small RNA-based immunity in insects. Current Opinion in Virology, 7, 19-28. doi:10.1016/j.coviro.2014.03.010

Burgyán, J., & Havelda, Z. (2011). Viral suppressors of RNA silencing. Trends in Plant Science, 16(5), 265-272. doi:10.1016/j.tplants.2011.02.010

Csorba, T., Kontra, L., & Burgyán, J. (2015). viral silencing suppressors: Tools forged to fine-tune host-pathogen coexistence. Virology, 479-480, 85-103. doi:10.1016/j.virol.2015.02.028

Pumplin, N., & Voinnet, O. (2013). RNA silencing suppression by plant pathogens: defence, counter-defence and counter-counter-defence. Nature Reviews Microbiology, 11(11), 745-760. doi:10.1038/nrmicro3120

Várallyay, É., & Havelda, Z. (2013). Unrelated viral suppressors of RNA silencing mediate the control of ARGONAUTE1 level. Molecular Plant Pathology, 14(6), 567-575. doi:10.1111/mpp.12029

Várallyay, É., Válóczi, A., Ágyi, Á., Burgyán, J., & Havelda, Z. (2010). Plant virus-mediated induction of miR168 is associated with repression of ARGONAUTE1 accumulation. The EMBO Journal, 29(20), 3507-3519. doi:10.1038/emboj.2010.215

Baumberger, N., Tsai, C.-H., Lie, M., Havecker, E., & Baulcombe, D. C. (2007). The Polerovirus Silencing Suppressor P0 Targets ARGONAUTE Proteins for Degradation. Current Biology, 17(18), 1609-1614. doi:10.1016/j.cub.2007.08.039

Csorba, T., Lózsa, R., Hutvágner, G., & Burgyán, J. (2010). Polerovirus protein P0 prevents the assembly of small RNA-containing RISC complexes and leads to degradation of ARGONAUTE1. The Plant Journal, 62(3), 463-472. doi:10.1111/j.1365-313x.2010.04163.x

Karran, R. A., & Sanfaçon, H. (2014). Tomato ringspot virus Coat Protein Binds to ARGONAUTE 1 and Suppresses the Translation Repression of a Reporter Gene. Molecular Plant-Microbe Interactions®, 27(9), 933-943. doi:10.1094/mpmi-04-14-0099-r

Pazhouhandeh, M., Dieterle, M., Marrocco, K., Lechner, E., Berry, B., Brault, V. r., … Ziegler-Graff, V. r. (2006). F-box-like domain in the polerovirus protein P0 is required for silencing suppressor function. Proceedings of the National Academy of Sciences, 103(6), 1994-1999. doi:10.1073/pnas.0510784103

Derrien, B., Baumberger, N., Schepetilnikov, M., Viotti, C., De Cillia, J., Ziegler-Graff, V., … Genschik, P. (2012). Degradation of the antiviral component ARGONAUTE1 by the autophagy pathway. Proceedings of the National Academy of Sciences, 109(39), 15942-15946. doi:10.1073/pnas.1209487109

Nayak, A., Berry, B., Tassetto, M., Kunitomi, M., Acevedo, A., Deng, C., … Andino, R. (2010). Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila. Nature Structural & Molecular Biology, 17(5), 547-554. doi:10.1038/nsmb.1810

Van Mierlo, J. T., Bronkhorst, A. W., Overheul, G. J., Sadanandan, S. A., Ekström, J.-O., Heestermans, M., … van Rij, R. P. (2012). Convergent Evolution of Argonaute-2 Slicer Antagonism in Two Distinct Insect RNA Viruses. PLoS Pathogens, 8(8), e1002872. doi:10.1371/journal.ppat.1002872

Mann, K. S., Johnson, K. N., Carroll, B. J., & Dietzgen, R. G. (2016). Cytorhabdovirus P protein suppresses RISC-mediated cleavage and RNA silencing amplification in planta. Virology, 490, 27-40. doi:10.1016/j.virol.2016.01.003

Giner, A., Lakatos, L., García-Chapa, M., López-Moya, J. J., & Burgyán, J. (2010). Viral Protein Inhibits RISC Activity by Argonaute Binding through Conserved WG/GW Motifs. PLoS Pathogens, 6(7), e1000996. doi:10.1371/journal.ppat.1000996

Vaucheret, H. (2008). Plant ARGONAUTES. Trends in Plant Science, 13(7), 350-358. doi:10.1016/j.tplants.2008.04.007

Mallory, A., & Vaucheret, H. (2010). Form, Function, and Regulation of ARGONAUTE Proteins. The Plant Cell, 22(12), 3879-3889. doi:10.1105/tpc.110.080671

Carbonell, A., & Carrington, J. C. (2015). Antiviral roles of plant ARGONAUTES. Current Opinion in Plant Biology, 27, 111-117. doi:10.1016/j.pbi.2015.06.013

Kertész, S., Kerényi, Z., Mérai, Z., Bartos, I., Pálfy, T., Barta, E., & Silhavy, D. (2006). Both introns and long 3′-UTRs operate as cis-acting elements to trigger nonsense-mediated decay in plants. Nucleic Acids Research, 34(21), 6147-6157. doi:10.1093/nar/gkl737

Carbonell, A., Fahlgren, N., Garcia-Ruiz, H., Gilbert, K. B., Montgomery, T. A., Nguyen, T., … Carrington, J. C. (2012). Functional Analysis of Three Arabidopsis ARGONAUTES Using Slicer-Defective Mutants  . The Plant Cell, 24(9), 3613-3629. doi:10.1105/tpc.112.099945

Cuperus, J. T., Carbonell, A., Fahlgren, N., Garcia-Ruiz, H., Burke, R. T., Takeda, A., … Carrington, J. C. (2010). Unique functionality of 22-nt miRNAs in triggering RDR6-dependent siRNA biogenesis from target transcripts in Arabidopsis. Nature Structural & Molecular Biology, 17(8), 997-1003. doi:10.1038/nsmb.1866

Montgomery, T. A., Howell, M. D., Cuperus, J. T., Li, D., Hansen, J. E., Alexander, A. L., … Carrington, J. C. (2008). Specificity of ARGONAUTE7-miR390 Interaction and Dual Functionality in TAS3 Trans-Acting siRNA Formation. Cell, 133(1), 128-141. doi:10.1016/j.cell.2008.02.033

Carbonell, A., Takeda, A., Fahlgren, N., Johnson, S. C., Cuperus, J. T., & Carrington, J. C. (2014). New Generation of Artificial MicroRNA and Synthetic Trans-Acting Small Interfering RNA Vectors for Efficient Gene Silencing in Arabidopsis. Plant Physiology, 165(1), 15-29. doi:10.1104/pp.113.234989

Terzi, L. C., & Simpson, G. G. (2009). Arabidopsis RNA immunoprecipitation. The Plant Journal, 59(1), 163-168. doi:10.1111/j.1365-313x.2009.03859.x

Li, F., Xu, D., Abad, J., & Li, R. (2012). Phylogenetic relationships of closely related potyviruses infecting sweet potato determined by genomic characterization of Sweet potato virus G and Sweet potato virus 2. Virus Genes, 45(1), 118-125. doi:10.1007/s11262-012-0749-2

Harvey, J. J. W., Lewsey, M. G., Patel, K., Westwood, J., Heimstädt, S., Carr, J. P., & Baulcombe, D. C. (2011). An Antiviral Defense Role of AGO2 in Plants. PLoS ONE, 6(1), e14639. doi:10.1371/journal.pone.0014639

Till, S., Lejeune, E., Thermann, R., Bortfeld, M., Hothorn, M., Enderle, D., … Ladurner, A. G. (2007). A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. Nature Structural & Molecular Biology, 14(10), 897-903. doi:10.1038/nsmb1302

Schirle, N. T., & MacRae, I. J. (2012). The Crystal Structure of Human Argonaute2. Science, 336(6084), 1037-1040. doi:10.1126/science.1221551

Elkayam, E., Kuhn, C.-D., Tocilj, A., Haase, A. D., Greene, E. M., Hannon, G. J., & Joshua-Tor, L. (2012). The Structure of Human Argonaute-2 in Complex with miR-20a. Cell, 150(1), 100-110. doi:10.1016/j.cell.2012.05.017

Hutvagner, G., & Simard, M. J. (2008). Argonaute proteins: key players in RNA silencing. Nature Reviews Molecular Cell Biology, 9(1), 22-32. doi:10.1038/nrm2321

Brown, K. M., Chu, C., & Rana, T. M. (2005). Target accessibility dictates the potency of human RISC. Nature Structural & Molecular Biology, 12(5), 469-470. doi:10.1038/nsmb931

Webster, L. C., Zhang, K., Chance, B., Ayene, I., Culp, J. S., Huang, W. J., … Ricciardi, R. P. (1991). Conversion of the E1A Cys4 zinc finger to a nonfunctional His2,Cys2 zinc finger by a single point mutation. Proceedings of the National Academy of Sciences, 88(22), 9989-9993. doi:10.1073/pnas.88.22.9989

Trinks, D., Rajeswaran, R., Shivaprasad, P. V., Akbergenov, R., Oakeley, E. J., Veluthambi, K., … Pooggin, M. M. (2005). Suppression of RNA Silencing by a Geminivirus Nuclear Protein, AC2, Correlates with Transactivation of Host Genes. Journal of Virology, 79(4), 2517-2527. doi:10.1128/jvi.79.4.2517-2527.2005

Chiba, S., Hleibieh, K., Delbianco, A., Klein, E., Ratti, C., Ziegler-Graff, V., … Gilmer, D. (2013). The Benyvirus RNA Silencing Suppressor Is Essential for Long-Distance Movement, Requires Both Zinc-Finger and NoLS Basic Residues but Not a Nucleolar Localization for Its Silencing-Suppression Activity. Molecular Plant-Microbe Interactions®, 26(2), 168-181. doi:10.1094/mpmi-06-12-0142-r

Bies‐Etheve, N., Pontier, D., Lahmy, S., Picart, C., Vega, D., Cooke, R., & Lagrange, T. (2009). RNA‐directed DNA methylation requires an AGO4‐interacting member of the SPT5 elongation factor family. EMBO reports, 10(6), 649-654. doi:10.1038/embor.2009.31

Chekulaeva, M., Filipowicz, W., & Parker, R. (2009). Multiple independent domains of dGW182 function in miRNA-mediated repression in Drosophila. RNA, 15(5), 794-803. doi:10.1261/rna.1364909

El-Shami, M., Pontier, D., Lahmy, S., Braun, L., Picart, C., Vega, D., … Lagrange, T. (2007). Reiterated WG/GW motifs form functionally and evolutionarily conserved ARGONAUTE-binding platforms in RNAi-related components. Genes & Development, 21(20), 2539-2544. doi:10.1101/gad.451207

He, X.-J., Hsu, Y.-F., Zhu, S., Wierzbicki, A. T., Pontes, O., Pikaard, C. S., … Zhu, J.-K. (2009). An Effector of RNA-Directed DNA Methylation in Arabidopsis Is an ARGONAUTE 4- and RNA-Binding Protein. Cell, 137(3), 498-508. doi:10.1016/j.cell.2009.04.028

Till, S., & Ladurner, A. G. (2007). RNA Pol IV Plays Catch with Argonaute 4. Cell, 131(4), 643-645. doi:10.1016/j.cell.2007.10.044

Bednenko, J., Noto, T., DeSouza, L. V., Siu, K. W. M., Pearlman, R. E., Mochizuki, K., & Gorovsky, M. A. (2009). Two GW Repeat Proteins Interact with Tetrahymena thermophila Argonaute and Promote Genome Rearrangement. Molecular and Cellular Biology, 29(18), 5020-5030. doi:10.1128/mcb.00076-09

Pontier, D., Picart, C., Roudier, F., Garcia, D., Lahmy, S., Azevedo, J., … Lagrange, T. (2012). NERD, a Plant-Specific GW Protein, Defines an Additional RNAi-Dependent Chromatin-Based Pathway in Arabidopsis. Molecular Cell, 48(1), 121-132. doi:10.1016/j.molcel.2012.07.027

Schirle, N. T., Sheu-Gruttadauria, J., & MacRae, I. J. (2014). Structural basis for microRNA targeting. Science, 346(6209), 608-613. doi:10.1126/science.1258040

GAMSJAEGER, R., LIEW, C., LOUGHLIN, F., CROSSLEY, M., & MACKAY, J. (2007). Sticky fingers: zinc-fingers as protein-recognition motifs. Trends in Biochemical Sciences, 32(2), 63-70. doi:10.1016/j.tibs.2006.12.007

Hall, T. M. T. (2005). Multiple modes of RNA recognition by zinc finger proteins. Current Opinion in Structural Biology, 15(3), 367-373. doi:10.1016/j.sbi.2005.04.004

Leung, A. K. L., Vyas, S., Rood, J. E., Bhutkar, A., Sharp, P. A., & Chang, P. (2011). Poly(ADP-Ribose) Regulates Stress Responses and MicroRNA Activity in the Cytoplasm. Molecular Cell, 42(4), 489-499. doi:10.1016/j.molcel.2011.04.015

Mukasa, S. B., Rubaihayo, P. R., & Valkonen, J. P. T. (2006). Interactions between a crinivirus, an ipomovirus and a potyvirus in coinfected sweetpotato plants. Plant Pathology, 55(3), 458-467. doi:10.1111/j.1365-3059.2006.01350.x

Ma, X., Nicole, M.-C., Meteignier, L.-V., Hong, N., Wang, G., & Moffett, P. (2014). Different roles for RNA silencing and RNA processing components in virus recovery and virus-induced gene silencing in plants. Journal of Experimental Botany, 66(3), 919-932. doi:10.1093/jxb/eru447

Garcia-Ruiz, H., Carbonell, A., Hoyer, J. S., Fahlgren, N., Gilbert, K. B., Takeda, A., … Carrington, J. C. (2015). Roles and Programming of Arabidopsis ARGONAUTE Proteins during Turnip Mosaic Virus Infection. PLOS Pathogens, 11(3), e1004755. doi:10.1371/journal.ppat.1004755




This item appears in the following Collection(s)

Show full item record