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A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC

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A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC

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dc.contributor.author Kenesi, Erzsebet es_ES
dc.contributor.author CARBONELL, ALBERTO es_ES
dc.contributor.author Lozsa, Rita es_ES
dc.contributor.author Vertessy, Beata es_ES
dc.contributor.author Lakatos, Lorant es_ES
dc.date.accessioned 2021-02-13T04:31:43Z
dc.date.available 2021-02-13T04:31:43Z
dc.date.issued 2017-07-27 es_ES
dc.identifier.issn 0305-1048 es_ES
dc.identifier.uri http://hdl.handle.net/10251/161199
dc.description.abstract [EN] In most eukaryotes, RNA silencing is an adaptive immune system regulating key biological processes including antiviral defense. To evade this response, viruses of plants, worms and insects have evolved viral suppressors of RNA silencing proteins (VSRs). Various VSRs, such as P1 from Sweet potato mild mottle virus (SPMMV), inhibit the activity of RNA-induced silencing complexes (RISCs) including an ARGONAUTE (AGO) protein loaded with a small RNA. However, the specific mechanisms explaining this class of inhibition are unknown. Here, we show that SPMMV P1 interacts with AGO1 and AGO2 from Arabidopsis thaliana, but solely interferes with AGO1 function. Moreover, a mutational analysis of a newly identified zinc finger domain in P1 revealed that this domain could represent an effector domain as it is required for P1 suppressor activity but not for AGO1 binding. Finally, a comparative analysis of the target RNA binding capacity of AGO1 in the presence of wild-type or suppressor-defective P1 forms revealed that P1 blocks target RNA binding to AGO1. Our results describe the negative regulation of RISC, the small RNA containing molecular machine. es_ES
dc.description.sponsorship Hungarian Scientific Research Fund (OTKA) [K91042, NN107787, NN11024 to L.L.]; European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska Curie [655841 to A.C.]. Funding for open access charge: OTKA [NN11024] es_ES
dc.language Inglés es_ES
dc.publisher Oxford University Press es_ES
dc.relation.ispartof Nucleic Acids Research es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject RNA silencing es_ES
dc.subject Plant virus, siRNA es_ES
dc.subject AGO es_ES
dc.subject Immunoprecipitation es_ES
dc.subject Silencing suppressor es_ES
dc.title A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/nar/gkx379 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/655841/EU/Genome-wide analysis of RNA and protein interacting profiles during a plant virus infection/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/OTKA//K91042/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/OTKA//NN107787/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/OTKA//NN11024/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Kenesi, E.; Carbonell, A.; Lozsa, R.; Vertessy, B.; Lakatos, L. (2017). A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC. Nucleic Acids Research. 45(13):7736-7750. https://doi.org/10.1093/nar/gkx379 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1093/nar/gkx379 es_ES
dc.description.upvformatpinicio 7736 es_ES
dc.description.upvformatpfin 7750 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 45 es_ES
dc.description.issue 13 es_ES
dc.identifier.pmid 28499009 es_ES
dc.identifier.pmcid PMC5737661 es_ES
dc.relation.pasarela S\375859 es_ES
dc.contributor.funder Hungarian Scientific Research Fund es_ES
dc.contributor.funder European Commission es_ES
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