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dc.contributor.author | Pla, Luis | es_ES |
dc.contributor.author | Aviñó, Anna | es_ES |
dc.contributor.author | Eritja, Ramón | es_ES |
dc.contributor.author | Ruiz-Gaitán, Alba | es_ES |
dc.contributor.author | Pemán, Javier | es_ES |
dc.contributor.author | Friaza, Vicente | es_ES |
dc.contributor.author | Calderón, Enrique J. | es_ES |
dc.contributor.author | Aznar, Elena | es_ES |
dc.contributor.author | Martínez-Máñez, Ramón | es_ES |
dc.contributor.author | Santiago Felipe, Sara | es_ES |
dc.date.accessioned | 2021-02-20T04:31:07Z | |
dc.date.available | 2021-02-20T04:31:07Z | |
dc.date.issued | 2020-12 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/161977 | |
dc.description.abstract | [EN] Pneumocystis pneumonia (PcP) is a disease produced by the opportunistic infection of the fungus Pneumocystis jirovecii. As delayed or unsuitable treatments increase the risk of mortality, the development of rapid and accurate diagnostic tools for PcP are of great importance. Unfortunately, current standard methods present severe limitations and are far from adequate. In this work, a time-competitive, sensitive and selective biosensor based on DNA-gated nanomaterials for the identification of P. jirovecii is presented. The biosensor consists of a nanoporous anodic alumina (NAA) scaffold which pores are filled with a dye reporter and capped with specific DNA oligonucleotides. In the presence of P. jirovecii genomic DNA, the gated biosensor is open, and the cargo is delivered to the solution where it is monitored through fluorescence spectroscopy. The use of capping oligonucleotides able to form duplex or triplex with P. jirovecii DNA is studied. The final diagnostic tool shows a limit of detection (LOD) of 1 nM of target complementary DNA and does not require previous amplification steps. The method was applied to identify DNA from P. jirovecii in unmodified bronchoalveolar lavage, nasopharyngeal aspirates, and sputum samples in 60 min. This is a promising alternative method for the routinely diagnosis of Pneumocystis pneumonia. | es_ES |
dc.description.sponsorship | This research was funded by the Spanish Government, (projects RTI2018-100910-B-C41 (MCUI/AEI/FEDER, UE) and CTQ2017-84415-R), the Generalitat Valenciana (project PROMETEO/2018/024) and CIBER-BBN (projects NANOPATH and CANDI-EYE). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation.ispartof | Journal of Fungi | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Nanoporous anodic alumina | es_ES |
dc.subject | Pneumocystis jirovecii | es_ES |
dc.subject | Molecular gates | es_ES |
dc.subject | Oligonucleotides | es_ES |
dc.subject | Biosensor | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.subject.classification | QUIMICA INORGANICA | es_ES |
dc.subject.classification | CIENCIA DE LOS MATERIALES E INGENIERIA METALURGICA | es_ES |
dc.title | Triplex hybridization-based nanosystem for the rapid screening of pneumocystis pneumonia in clinical samples | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/jof6040292 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2017-84415-R/ES/ESTUDIO DE LAS ESTRUCTURAS DE ADN CON POTENCIAL BIOMEDICO/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2018%2F024/ES/Sistemas avanzados de liberación controlada/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-100910-B-C41/ES/MATERIALES POROSOS INTELIGENTES MULTIFUNCIONALES Y DISPOSITIVOS ELECTRONICOS PARA LA LIBERACION DE FARMACOS, DETECCION DE DROGAS Y BIOMARCADORES Y COMUNICACION A NANOESCALA/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.description.bibliographicCitation | Pla, L.; Aviñó, A.; Eritja, R.; Ruiz-Gaitán, A.; Pemán, J.; Friaza, V.; Calderón, EJ.... (2020). Triplex hybridization-based nanosystem for the rapid screening of pneumocystis pneumonia in clinical samples. Journal of Fungi. 6(4):1-14. https://doi.org/10.3390/jof6040292 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/jof6040292 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 14 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 6 | es_ES |
dc.description.issue | 4 | es_ES |
dc.identifier.eissn | 2309-608X | es_ES |
dc.relation.pasarela | S\423690 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina | es_ES |
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