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Gold Nanoparticle-Assisted Virus Formation by Means of the Delivery of an Oncolytic Adenovirus Genome

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Gold Nanoparticle-Assisted Virus Formation by Means of the Delivery of an Oncolytic Adenovirus Genome

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Nombre: Sendra;Miguel;Nav ...
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dc.contributor.author Sendra, Luis es_ES
dc.contributor.author Miguel, Antonio es_ES
dc.contributor.author Navarro-Plaza, M. Carmen es_ES
dc.contributor.author Herrero, María José es_ES
dc.contributor.author de la Higuera, José es_ES
dc.contributor.author Cháfer-Pericás, Consuelo es_ES
dc.contributor.author Aznar, Elena es_ES
dc.contributor.author Marcos Martínez, María Dolores es_ES
dc.contributor.author Martínez-Máñez, Ramón es_ES
dc.contributor.author Rojas, Luis Alfonso es_ES
dc.contributor.author Alemany, Ramón es_ES
dc.contributor.author Aliño, Salvador F. es_ES
dc.date.accessioned 2021-02-25T04:49:29Z
dc.date.available 2021-02-25T04:49:29Z
dc.date.issued 2020-06 es_ES
dc.identifier.uri http://hdl.handle.net/10251/162367
dc.description.abstract [EN] Oncolytic adenoviruses are a therapeutic alternative to treat cancer based on their ability to replicate selectively in tumor cells. However, their use is limited mainly by the neutralizing antibody (Nab) immune response that prevents repeated dosing. An alternative to facilitate the DNA access to the tumor even in the presence of anti-viral Nabs could be gold nanoparticles able to transfer DNA molecules. However, the ability of these nanoparticles to carry large DNA molecules, such as an oncolytic adenovirus genome, has not been studied. In this work, gold nanoparticles were functionalized with different amounts of polyethylenimine to transfer in a safe and efficient manner a large oncolytic virus genome. Their transfer efficacy and final effect of the oncolytic virus in cancer cells are studied. For each synthesized nanoparticle, (a) DNA loading capacity, (b) complex size, (c) DNA protection ability, (d) transfection efficacy and (e) cytotoxic effect were studied. We observed that small gold nanoparticles (70-80 nm in diameter) protected DNA against nucleases and were able to transfect the ICOVIR-15 oncolytic virus genome encoded in pLR1 plasmid. In the present work, efficient transgene RNA expression, luciferase activity and viral cytopathic effect on cancer cells are reported. These results suggest gold nanoparticles to be an efficient and safe vector for oncolytic adenovirus genome transfer. es_ES
dc.description.sponsorship This research was supported by University of Valencia 'Ayuda a la Investigacion', Asociacion Pablo Ugarte and European Regional Development Fund (VLC-CAMPUS). es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Nanomaterials es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Gold nanoparticles es_ES
dc.subject Delivery es_ES
dc.subject Gene therapy es_ES
dc.subject Non-viral vectors es_ES
dc.subject Oncolytic virus es_ES
dc.subject Virotherapy es_ES
dc.subject Cancer es_ES
dc.subject.classification QUIMICA INORGANICA es_ES
dc.subject.classification CIENCIA DE LOS MATERIALES E INGENIERIA METALURGICA es_ES
dc.title Gold Nanoparticle-Assisted Virus Formation by Means of the Delivery of an Oncolytic Adenovirus Genome es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/nano10061183 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Sendra, L.; Miguel, A.; Navarro-Plaza, MC.; Herrero, MJ.; De La Higuera, J.; Cháfer-Pericás, C.; Aznar, E.... (2020). Gold Nanoparticle-Assisted Virus Formation by Means of the Delivery of an Oncolytic Adenovirus Genome. Nanomaterials. 10(6):1-16. https://doi.org/10.3390/nano10061183 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/nano10061183 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 16 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 10 es_ES
dc.description.issue 6 es_ES
dc.identifier.eissn 2079-4991 es_ES
dc.identifier.pmid 32560474 es_ES
dc.identifier.pmcid PMC7353451 es_ES
dc.relation.pasarela S\418274 es_ES
dc.contributor.funder Asociación Pablo Ugarte es_ES
dc.contributor.funder Universitat de València es_ES
dc.contributor.funder European Regional Development Fund es_ES
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