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How sequence variants of a plastid-replicating viroid with one single nucleotide change initiate disease in its natural host

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How sequence variants of a plastid-replicating viroid with one single nucleotide change initiate disease in its natural host

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dc.contributor.author Delgado Villar, Sonia Guadalupe es_ES
dc.contributor.author Navarro, Beatriz es_ES
dc.contributor.author Serra, Pedro es_ES
dc.contributor.author Gentit, Pascal es_ES
dc.contributor.author Cambra, Miguel Ángel es_ES
dc.contributor.author Chiumenti, Michela es_ES
dc.contributor.author De Stradis, Angelo es_ES
dc.contributor.author Di Serio, Francesco es_ES
dc.contributor.author FLORES PEDAUYE, RICARDO es_ES
dc.date.accessioned 2021-03-01T08:09:26Z
dc.date.available 2021-03-01T08:09:26Z
dc.date.issued 2019-07-03 es_ES
dc.identifier.issn 1547-6286 es_ES
dc.identifier.uri http://hdl.handle.net/10251/162588
dc.description.abstract [EN] Understanding how viruses and subviral agents initiate disease is central to plant pathology. Whether RNA silencing mediates the primary lesion triggered by viroids (small non-protein-coding RNAs), or just intermediate-late steps of a signaling cascade, remains unsolved. While most variants of the plastid-replicating peach latent mosaic viroid (PLMVd) are asymptomatic, some incite peach mosaics or albinism (peach calico, PC). We have previously shown that two 21-nt small RNAs (PLMVd-sRNAs) containing a 12-13-nt PC-associated insertion guide cleavage, via RNA silencing, of the mRNA encoding a heat-shock protein involved in chloroplast biogenesis. To gain evidence supporting that such event is the initial lesion, and more specifically, that different chloroses have different primary causes, here we focused on a PLMVd-induced peach yellow mosaic (PYM) expressed in leaf sectors interspersed with others green. First, sequencing PLMVd-cDNAs from both sectors and bioassays mapped the PYM determinant at one nucleotide, a notion further sustained by the phenotype incited by other natural and artificial PLMVd variants. And second, sRNA deep-sequencing and RNA ligase-mediated RACE identified one PLMVd-sRNA with the PYM-associated change that guides cleavage, as predicted by RNA silencing, of the mRNA encoding a thylakoid translocase subunit required for chloroplast development. RT-qPCR showed lower accumulation of this mRNA in PYM-expressing tissues. Remarkably, PLMVd-sRNAs triggering PYM and PC have 5MODIFIER LETTER PRIME-terminal Us, involving Argonaute 1 in what likely are the initial alterations eliciting distinct chloroses. es_ES
dc.description.sponsorship This work was funded by grant BFU2014-56812-P (to R.F.) from the Ministerio de Economia y Competitividad of Spain (MINECO). S.D. and P.S. were partly supported by postdoctoral contracts from MINECO. We thank the technical assistance of Amparo Ahuir and Maria Pedrote in handling the plants. We apologize to colleagues whose work was not quoted for space limitations. es_ES
dc.language Inglés es_ES
dc.publisher Landes Bioscience es_ES
dc.relation.ispartof RNA Biology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Chloroplast biogenesis es_ES
dc.subject Non-protein-coding RNAs es_ES
dc.subject PLMVd es_ES
dc.subject RNA silencing es_ES
dc.subject Thylakoid translocase es_ES
dc.subject Viroid pathogenesis es_ES
dc.title How sequence variants of a plastid-replicating viroid with one single nucleotide change initiate disease in its natural host es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1080/15476286.2019.1600396 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2014-56812-P/ES/VIROIDES, LOS PARASITOS EXTREMOS: EVOLUCION ESPACIO-TEMPORAL, PATOGENESIS MEDIADA POR SILENCIAMIENTO VIA RNA, Y DEGRADACION/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Delgado Villar, SG.; Navarro, B.; Serra, P.; Gentit, P.; Cambra, MÁ.; Chiumenti, M.; De Stradis, A.... (2019). How sequence variants of a plastid-replicating viroid with one single nucleotide change initiate disease in its natural host. RNA Biology. 16(7):906-917. https://doi.org/10.1080/15476286.2019.1600396 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1080/15476286.2019.1600396 es_ES
dc.description.upvformatpinicio 906 es_ES
dc.description.upvformatpfin 917 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 16 es_ES
dc.description.issue 7 es_ES
dc.identifier.pmid 30990352 es_ES
dc.identifier.pmcid PMC6546372 es_ES
dc.relation.pasarela S\406820 es_ES
dc.contributor.funder Ministerio de Ciencia, Innovación y Universidades es_ES
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