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Aptamer-Capped nanoporous anodic alumina for Staphylococcus aureus detection

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Aptamer-Capped nanoporous anodic alumina for Staphylococcus aureus detection

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dc.contributor.author Pla, Luis es_ES
dc.contributor.author Santiago Felipe, Sara es_ES
dc.contributor.author Tormo-Mas, María Ángeles es_ES
dc.contributor.author Pemán, Javier es_ES
dc.contributor.author Sancenón Galarza, Félix es_ES
dc.contributor.author Aznar, Elena es_ES
dc.contributor.author Martínez-Máñez, Ramón es_ES
dc.date.accessioned 2021-03-16T04:31:10Z
dc.date.available 2021-03-16T04:31:10Z
dc.date.issued 2020-10-01 es_ES
dc.identifier.issn 0925-4005 es_ES
dc.identifier.uri http://hdl.handle.net/10251/163876
dc.description.abstract [EN] The development of new detection systems for an accurate and rapid identification of pathogens has become an essential challenge in the biomedical field. Herein a highly selective platform based on aptamer-gated nano materials for specific Staphylococcus aureus detection is presented. In the proposed design, a nanoporous anodic alumina (NAA) scaffold is loaded with the fluorescent indicator rhodamine B, while pores entrances are capped by a DNA aptamer which selectively recognizes S. aureus cells in less than 1 h. When S. aureus cells are present, the solid is selectively uncapped, and the dye is released to the medium. This nanodevice allows the detection of bacterial concentrations between 2 and 5 CFU mL-1 (in buffer and blood, respectively) and it has demonstrated excellent behavior in terms of specificity and robustness. A set of 25 different clinical samples are analyzed using this simple procedure obtaining excellent results, which agree with conventional hospital reference techniques for the identification of S. aureus. This new method is sensitive, rapid and low cost, and avoids steps such as polymerase chain amplification reaction, which makes it suitable for use in point-of-care detection systems. es_ES
dc.description.sponsorship This study was supported by the Spanish Government (projects RTI2018-100910-B-C41 and SAF2017-82251-R (MCUI/AEI/FEDER, UE)), the Generalitat Valenciana (project PROMETEO/2018/024), the Universitat Politecnica de Valencia-Instituto de Investigacion Sanitaria La Fe (B02-MIRSA project) and CIBER-BBN (NANOPATH and valorization project CANDI-EYE). It has been also co-financed by the EU through the Valencian Community ERDF PO 2014-2020. S.S. thanks the Instituto de Salud Carlos III and the European Social Fund for the financial support "Sara Borrell" (CD16/000237). L.P. thanks to Ministerio de Economia, Industria y Competitividad for his FPI grant. The authors are grateful for the professional English language editing to Mr. Arash Javadinejad, English Instructor and pubication Editor at the Instituto de Investigacion Sanitaria La Fe, Valencia, Spain. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Sensors and Actuators B Chemical es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Aptamers es_ES
dc.subject Gated materials es_ES
dc.subject Staphylococcus aureus es_ES
dc.subject Optical sensors es_ES
dc.subject.classification QUIMICA ORGANICA es_ES
dc.subject.classification QUIMICA INORGANICA es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Aptamer-Capped nanoporous anodic alumina for Staphylococcus aureus detection es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.snb.2020.128281 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2017-82251-R/ES/NUEVAS ESTRATEGIAS DE PREVENCION Y DIAGNOSTICO DE LAS INFECCIONES RELACIONADAS CON DISPOSITIVOS BIOMEDICOS CAUSADOS POR STAPHYLOCOCCUS SPP/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/IISLAFE//B02-MIRSA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//CD16%2F00237/ES/CD16%2F00237/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2018%2F024/ES/Sistemas avanzados de liberación controlada/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-100910-B-C41/ES/MATERIALES POROSOS INTELIGENTES MULTIFUNCIONALES Y DISPOSITIVOS ELECTRONICOS PARA LA LIBERACION DE FARMACOS, DETECCION DE DROGAS Y BIOMARCADORES Y COMUNICACION A NANOESCALA/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Pla, L.; Santiago Felipe, S.; Tormo-Mas, MÁ.; Pemán, J.; Sancenón Galarza, F.; Aznar, E.; Martínez-Máñez, R. (2020). Aptamer-Capped nanoporous anodic alumina for Staphylococcus aureus detection. Sensors and Actuators B Chemical. 320(128281). https://doi.org/10.1016/j.snb.2020.128281 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.snb.2020.128281 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 320 es_ES
dc.description.issue 128281 es_ES
dc.relation.pasarela S\418487 es_ES
dc.contributor.funder European Social Fund es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
dc.contributor.funder Instituto de Investigación Sanitaria La Fe es_ES
dc.contributor.funder Ministerio de Ciencia, Innovación y Universidades es_ES
dc.contributor.funder Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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