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Long-Term Phenotypic and Proteomic Changes Following Vitrified Embryo Transfer in the Rabbit Model

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Long-Term Phenotypic and Proteomic Changes Following Vitrified Embryo Transfer in the Rabbit Model

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dc.contributor.author Garcia-Dominguez, X es_ES
dc.contributor.author Marco-Jiménez, Francisco es_ES
dc.contributor.author Peñaranda, D.S. es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.date.accessioned 2021-04-22T03:31:15Z
dc.date.available 2021-04-22T03:31:15Z
dc.date.issued 2020-06 es_ES
dc.identifier.uri http://hdl.handle.net/10251/165476
dc.description.abstract [EN] This study was conducted to demonstrate how a vitrified embryo transfer procedure incurs phenotypic and molecular changes throughout life. This study reports the first evidence describing that embryonic manipulation during a vitrified embryo transfer cycle induced molecular modifications, concerning oxidative phosphorylation and dysregulations in zinc and lipid metabolism in liver tissue, which has been reported as responsible for postnatal variations of the phenotype. Nowadays, assisted reproductive technologies (ARTs) are considered valuable contributors to our past, but a future without their use is inconceivable. However, in recent years, several studies have evidenced a potential impact of ART on long-term development in mammal species. To date, the long-term follow-up data are still limited. So far, studies have mainly focused on in vitro fertilization or in vitro culture, with information from gametes/embryos cryopreservation field being practically missing. Herein, we report an approach to determine whether a vitrified embryo transfer procedure would have long-term consequences on the offspring. Using the rabbit as a model, we compared animals derived from vitrified-transferred embryos versus those naturally conceived, studying the growth performance, plus the weight throughout life, and the internal organs/tissues phenotype. The healthy status was assessed over the hematological and biochemical parameters in peripheral blood. Additionally, a comparative proteomic analysis was conducted in the liver tissue to investigate molecular cues related to vitrified embryo transfer in an adult tissue. After vitrified embryo transfer, birth weight was increased, and the growth performance was diminished in a sex-specific manner. In addition, vitrified-transferred animals showed significantly lower body, liver and heart weights in adulthood. Molecular analyses revealed that vitrified embryo transfer triggers reprogramming of the liver proteome. Functional analysis of the differentially expressed proteins showed changes in relation to oxidative phosphorylation and dysregulations in the zinc and lipid metabolism, which has been reported as possible causes of a disturbed growth pattern. Therefore, we conclude that vitrified embryo transfer is not a neutral procedure, and it incurs long-term effects in the offspring both at phenotypic and molecular levels. These results described a striking example of the developmental plasticity exhibited by the mammalian embryo. es_ES
dc.description.sponsorship Funding from the Ministry of Economy, Industry and Competitiveness (Research project: AGL2017-85162-C2-1-R and AGL2014-53405-C2-1-P) is acknowledged. X.G.D. was supported by a research grant from the Ministry of Economy, Industry and Competitiveness (BES-2015-072429). es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Animals es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Assisted reproduction technology es_ES
dc.subject Embryo vitrification es_ES
dc.subject Embryo transfer es_ES
dc.subject Postnatal outcomes es_ES
dc.subject Proteome es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Long-Term Phenotypic and Proteomic Changes Following Vitrified Embryo Transfer in the Rabbit Model es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/ani10061043 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2014-53405-C2-1-P/ES/MEJORA GENETICA DEL CONEJO DE CARNE:RESPUESTA A LA SELECCION Y SU EFECTO SOBRE LA REPRODUCCION, ALIMENTACION Y SALUD UTILIZANDO UNA POBLACION CONTROL CRIOCONSERVADA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BES-2015-072429/ES/BES-2015-072429/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-85162-C2-1-R/ES/MEJORA GENETICA DEL CONEJO DE CARNE: ESTRATEGIAS PARA INCREMENTAR LA EFICACIA DE LA MEJORA, REPRODUCCION Y SALUD DE LINEAS PATERNALES/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Garcia-Dominguez, X.; Marco-Jiménez, F.; Peñaranda, D.; Vicente Antón, JS. (2020). Long-Term Phenotypic and Proteomic Changes Following Vitrified Embryo Transfer in the Rabbit Model. Animals. 10(6):1-16. https://doi.org/10.3390/ani10061043 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/ani10061043 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 16 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 10 es_ES
dc.description.issue 6 es_ES
dc.identifier.eissn 2076-2615 es_ES
dc.identifier.pmid 32560425 es_ES
dc.identifier.pmcid PMC7341293 es_ES
dc.relation.pasarela S\414090 es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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