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A Ciliary Motility Index for Activity Measurement in Cell Cultures With Respiratory Syncytial Virus

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A Ciliary Motility Index for Activity Measurement in Cell Cultures With Respiratory Syncytial Virus

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Nombre: Parrlla;Armengot;Mata ...
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dc.contributor.author Parrilla Bernabé, Eduardo es_ES
dc.contributor.author Armengot, Miguel es_ES
dc.contributor.author Mata, Manuel es_ES
dc.contributor.author Carda, Carmen es_ES
dc.contributor.author Cortijo, Julio es_ES
dc.contributor.author Moratal, David es_ES
dc.contributor.author Ginestar Peiro, Damián es_ES
dc.contributor.author Hueso, José L. es_ES
dc.contributor.author Riera Guasp, Jaime es_ES
dc.date.accessioned 2021-04-23T03:31:58Z
dc.date.available 2021-04-23T03:31:58Z
dc.date.issued 2019-03-01 es_ES
dc.identifier.issn 1945-8924 es_ES
dc.identifier.uri http://hdl.handle.net/10251/165524
dc.description.abstract [EN] Background: The respiratory epithelium is frequently infected by the respiratory syncytial virus, resulting in inflammation, a reduction in cilia activity and an increase in the production of mucus. Methods: In this study, an automatic method has been proposed to characterize the ciliary motility from cell cultures by means of a motility index using a dense optical flow algorithm. This method allows us to determine the ciliary beat frequency (CBF) together with a ciliary motility index of the cells in the cultures. The object of this analysis is to automatically distinguish between normal and infected cells in a culture. Results: The method was applied in 2 stages. It was concluded from the first stage that the CBF is not a good enough indicator to discriminate between the control and infected cultures. However, the ciliary motility index does succeed in discriminating between the control and infected cultures using the t test with a value t ¿ 6.46 and P <.001. In the second stage, it has been shown that the ciliary motility index did not differ significantly between patients, and the analysis of variance test gives a ¿ 0.05, F ¿ 1.61, P ¿.20. A threshold for this index has been determined using a receiver operating characteristics analysis that gives an area under the curve of 0.93. Conclusions: We have obtained a ciliary motility index that is able to discriminate between control and infected cultures after the eighth postinfection day. After infection, there is a rapid cilia loss of the cells and the measured CBF corresponds to the remaining noninfected cells. This is why the CBF does not discriminate between the control and the infected cells. es_ES
dc.description.sponsorship The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by grants MAT2016-76039-C4-2-R (C.C.) and PI16/01315 (M.M.) from the Ministry of Economy and Competitiveness of the Spanish Government and by the Instituto de Salud Carlos III (ISCIII). CIBER-BBN and CIBERER are funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, and CIBER Actions and financed by the ISCIII with the assistance of the European Regional Development Fund. es_ES
dc.language Inglés es_ES
dc.publisher SAGE Publishing es_ES
dc.relation.ispartof American Journal of Rhinology and Allergy es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Respiratory epithelium es_ES
dc.subject Respiratory syncytial virus es_ES
dc.subject Dense optical flow es_ES
dc.subject Ciliary motility: Cell cultures es_ES
dc.subject Ciliary beat frequency es_ES
dc.subject Ciliary beat pattern es_ES
dc.subject Motility index es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.subject.classification MATEMATICA APLICADA es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title A Ciliary Motility Index for Activity Measurement in Cell Cultures With Respiratory Syncytial Virus es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1177/1945892418811324 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2016-76039-C4-2-R/ES/DIFERENCIACION CONDROGENICA DE CELULAS CULTIVADAS EN INTERFASES ELECTRICAMENTE ACTIVAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//PI16%2F01315/ES/Sustitutos traqueales epitelizados generados por ingeniería tisular/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Matemática Aplicada - Departament de Matemàtica Aplicada es_ES
dc.description.bibliographicCitation Parrilla Bernabé, E.; Armengot, M.; Mata, M.; Carda, C.; Cortijo, J.; Moratal, D.; Ginestar Peiro, D.... (2019). A Ciliary Motility Index for Activity Measurement in Cell Cultures With Respiratory Syncytial Virus. American Journal of Rhinology and Allergy. 33(2):121-128. https://doi.org/10.1177/1945892418811324 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1177/1945892418811324 es_ES
dc.description.upvformatpinicio 121 es_ES
dc.description.upvformatpfin 128 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 33 es_ES
dc.description.issue 2 es_ES
dc.identifier.pmid 30457015 es_ES
dc.relation.pasarela S\381010 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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