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Pathogenesis of Intradermal Staphylococcal Infections Rabbit Experimental Approach to Natural Staphylococcus aureus Skin Infections

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Pathogenesis of Intradermal Staphylococcal Infections Rabbit Experimental Approach to Natural Staphylococcus aureus Skin Infections

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dc.contributor.author Muñoz-Silvestre, A. es_ES
dc.contributor.author Penadés, Mariola es_ES
dc.contributor.author Selva, L. es_ES
dc.contributor.author Pérez-Fuentes, S. es_ES
dc.contributor.author Moreno Grua, E. es_ES
dc.contributor.author García-Quirós, Ana es_ES
dc.contributor.author Pascual Amorós, Juan José es_ES
dc.contributor.author Arnau-Bonachera, Alberto es_ES
dc.contributor.author Barragán, A. es_ES
dc.contributor.author CORPA, JUAN MANUEL es_ES
dc.contributor.author Viana, David es_ES
dc.date.accessioned 2021-04-24T03:31:21Z
dc.date.available 2021-04-24T03:31:21Z
dc.date.issued 2020-06 es_ES
dc.identifier.issn 0002-9440 es_ES
dc.identifier.uri http://hdl.handle.net/10251/165563
dc.description.abstract [EN] Despite the enormous efforts made to achieve effective tools that fight against Staphylococcus aureus, the results have not been successful. This failure may be due to the absence of truly representative experimental models. To overcome this deficiency, the present work describes and immunologically characterizes the infection for 28 days, in an experimental low-dose (300 colony-forming units) intradermal model of infection in rabbits, which reproduces the characteristic staphylococcal abscess. Surprisingly, when mutant strains in the genes involved in virulence (J Delta agr, J Delta coa Delta vwb, J Delta hla, and J Delta psm alpha) were inoculated, no strong effect on the severity of lesions was observed, unlike other models that use high doses of bacteria. The inoculation of a human rabbitized (FdltB(r)) strain demonstrated its capacity to generate a similar inflammatory response to a wild-type rabbit strain and, therefore, validated this model for conducting these experimental studies with human strains. To conclude, this model proved reproducible and may be an option of choice to check both wild-type and mutant strains of different origins. es_ES
dc.description.sponsorship Supported by the Spanish Ministry of Economy and Competitiveness (MINECO) grant AGL2014-53405-C2-2-P, the Universidad CEU Cardenal Herrera, and European Union Development Fund (FEDER) Programme PO FEDER 2007-2013. A.M.-S., A.G.-Q., and E.M.-G. receive fellowship support from the Universidad CEU Cardenal Herrera; S.P.-F. receives fellowship support from the Generalitat Valenciana ACIF/2016/085; and M.P. and E.M.-G. receive fellowship support from the Spanish Ministry of Education, Culture, and Sport AP2010-3907 and FPU17/02708, respectively. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof The American Journal of Pathology es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Phenol-Soluble modulins es_ES
dc.subject Virulence determinants es_ES
dc.subject Toxin es_ES
dc.subject Expression es_ES
dc.subject Animals es_ES
dc.subject Models es_ES
dc.subject Immune es_ES
dc.subject Gene es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Pathogenesis of Intradermal Staphylococcal Infections Rabbit Experimental Approach to Natural Staphylococcus aureus Skin Infections es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.ajpath.2020.01.019 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2014-53405-C2-1-P/ES/MEJORA GENETICA DEL CONEJO DE CARNE:RESPUESTA A LA SELECCION Y SU EFECTO SOBRE LA REPRODUCCION, ALIMENTACION Y SALUD UTILIZANDO UNA POBLACION CONTROL CRIOCONSERVADA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2014-53405-C2-2-P/ES/CARACTERIZACION INMUNOLOGICA Y RESPUESTA INMUNE A RETOS INFECCIOSOS EN CONEJOS SEPARADOS POR 15 AÑOS DE SELECCION POR TASA DE CRECIMIENTO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACIF%2F2016%2F085/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MECD//AP2010-3907/ES/AP2010-3907/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MECD//FPU17%2F02708/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Muñoz-Silvestre, A.; Penadés, M.; Selva, L.; Pérez-Fuentes, S.; Moreno Grua, E.; García-Quirós, A.; Pascual Amorós, JJ.... (2020). Pathogenesis of Intradermal Staphylococcal Infections Rabbit Experimental Approach to Natural Staphylococcus aureus Skin Infections. The American Journal of Pathology. 190(6):1188-1210. https://doi.org/10.1016/j.ajpath.2020.01.019 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.ajpath.2020.01.019 es_ES
dc.description.upvformatpinicio 1188 es_ES
dc.description.upvformatpfin 1210 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 190 es_ES
dc.description.issue 6 es_ES
dc.identifier.pmid 32201266 es_ES
dc.relation.pasarela S\424797 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Universidad CEU Cardenal Herrera es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Ministerio de Educación, Cultura y Deporte es_ES
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dc.subject.ods 08.- Fomentar el crecimiento económico sostenido, inclusivo y sostenible, el empleo pleno y productivo, y el trabajo decente para todos es_ES


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