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Conduits based on the combination of hyaluronic acid and silk fibroin: Characterization, in vitro studies and in vivo biocompatibility

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Conduits based on the combination of hyaluronic acid and silk fibroin: Characterization, in vitro studies and in vivo biocompatibility

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dc.contributor.author Gisbert-Roca, Fernando es_ES
dc.contributor.author Lozano Picazo, Paloma es_ES
dc.contributor.author Pérez-Rigueiro, José es_ES
dc.contributor.author Guinea Tortuero, Gustavo Victor es_ES
dc.contributor.author Monleón Pradas, Manuel es_ES
dc.contributor.author Martínez-Ramos, Cristina es_ES
dc.date.accessioned 2021-04-27T03:32:35Z
dc.date.available 2021-04-27T03:32:35Z
dc.date.issued 2020-04-01 es_ES
dc.identifier.issn 0141-8130 es_ES
dc.identifier.uri http://hdl.handle.net/10251/165600
dc.description.abstract [EN] We address the production of structures intended as conduits made from natural biopolymers, capable of promoting the regeneration of axonal tracts. We combine hyaluronic acid (HA) and silk fibroin (SF) with the aim of improving mechanical and biological properties of HA. The results show that SF can be efficiently incorporated into the production process, obtaining conduits with tubular structure with a matrix of HA-SF blend. HA-SF has better mechanical properties than sole HA, which is a very soft hydrogel, facilitating manipulation. Culture of rat Schwann cells shows that cell adhesion and proliferation are higher than in pure HA, maybe due to the binding motifs contributed by the SF protein. This increased proliferation accelerates the formation of a tight cell layer, which covers the inner channel surface of the HA-SF tubes. Biocompatibility of the scaffolds was studied in immunocompetent mice. Both HA and HA-SF scaffolds were accepted by the host with no residual immune response at 8 weeks. New collagen extracellular matrix and new blood vessels were visible and they were present earlier when SF was present. The results show that incorporation of SF enhances the mechanical properties of the materials and results in promising biocompatible conduits for tubulization strategies. es_ES
dc.description.sponsorship The authors acknowledge financing from the Spanish Ministry of Economy and Competitiveness through grants RTI2018-095872-B-C22/ERDF, DPI2015-72863-EXP, MAT2016-79832-R, MAT2016-76847-R and Community of Madrid through grant Neurocentro-B2017/BMD-3760. FGR acknowledges scholarship FPU16/01833 of the Spanish Ministry of Education, Culture and Sports. We thank the Electron Microscopy Service at the UPV, where the FESEM images were obtained es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation MINECO/MAT2016-79832-R es_ES
dc.relation MINECO/MAT2016-76847-R es_ES
dc.relation CAM/Neurocentro-B2017/BMD-3760 es_ES
dc.relation info:eu-repo/grantAgreement/MINECO//DPI2015-72863-EXP/ES/NEUROCABLES MODULARES: MULTIPLICANDO CONEXIONES NEURALES/ es_ES
dc.relation MECYD/FPU16/01833 es_ES
dc.relation info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095872-B-C22/ES/NUEVO DISPOSITIVO BIOACTIVO PARA LA REGENERACION DE LESIONES DE LA MEDULA ESPINAL./ es_ES
dc.relation.ispartof International Journal of Biological Macromolecules es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Biomaterials es_ES
dc.subject Hyaluronic acid es_ES
dc.subject Silk fibroin es_ES
dc.subject Tissue engineering es_ES
dc.subject Nerve guidance conduits es_ES
dc.subject.classification TERMODINAMICA APLICADA (UPV) es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title Conduits based on the combination of hyaluronic acid and silk fibroin: Characterization, in vitro studies and in vivo biocompatibility es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.ijbiomac.2020.01.149 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Gisbert-Roca, F.; Lozano Picazo, P.; Pérez-Rigueiro, J.; Guinea Tortuero, GV.; Monleón Pradas, M.; Martínez-Ramos, C. (2020). Conduits based on the combination of hyaluronic acid and silk fibroin: Characterization, in vitro studies and in vivo biocompatibility. International Journal of Biological Macromolecules. 148:378-390. https://doi.org/10.1016/j.ijbiomac.2020.01.149 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.ijbiomac.2020.01.149 es_ES
dc.description.upvformatpinicio 378 es_ES
dc.description.upvformatpfin 390 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 148 es_ES
dc.identifier.pmid 31954793 es_ES
dc.relation.pasarela S\404524 es_ES
dc.contributor.funder Comunidad de Madrid es_ES
dc.contributor.funder Ministerio de Educación es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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