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Design and characterization of microspheres for a 3D mesenchymal stem cell culture

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Design and characterization of microspheres for a 3D mesenchymal stem cell culture

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dc.contributor.author Lastra, María Laura es_ES
dc.contributor.author Gómez Ribelles, José Luís es_ES
dc.contributor.author Cortizo, Ana María es_ES
dc.date.accessioned 2021-04-30T03:31:45Z
dc.date.available 2021-04-30T03:31:45Z
dc.date.issued 2020-12 es_ES
dc.identifier.issn 0927-7765 es_ES
dc.identifier.uri http://hdl.handle.net/10251/165805
dc.description.abstract [EN] Recent studies have shown the relevance of growing mesenchymal stem cells (MSCs) in three-dimensional environments with respect to the monolayer cell culture on an adherent substrate. In this sense, macroporous scaffolds and hydrogels have been used as three-dimensional (3D) supports. In this work, we explored the culture of MSCs in a 3D environment created by microspheres, prepared with a fumarate-vinyl acetate copolymer and chitosan. In this system, the environment that the cells feel has similarities to that found by the cells encapsulated in a hydrogel, but the cells have the ability to reorganize their environment since the microspheres are mobile. We evaluated their biocompatibility in vitro using RAW 264.7 macrophages and bone marrow mesenchymal stem cells (BMSCs). The results with RAW 264.7 cells showed good cell viability, without evident signs of cytotoxicity. BMSCs not only proliferate, but also rearrange to grow in clusters, thus highlighting the advantages of microspheres as 3D environments. es_ES
dc.description.sponsorship This work supported by Universidad Nacional de La Plata (11/X 768 and subsidio Jovenes Investigadores 2017), Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. In addition, financial support from the Spanish State Research Agency (AEI) and the European Regional Development Fund (ERFD) through the project PID2019-106099RB-C41/AEI/10.13039/501100011033 is acknowledged. CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER Actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. MLL is a Posdoctoral Fellow of CONICET, AMC is a member of Carrera del Investigador Cientifico de la CICPBA. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Colloids and Surfaces B Biointerfaces es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Microspheres es_ES
dc.subject Chitosan es_ES
dc.subject 3D culture environment es_ES
dc.subject Mesenchymal stem cells es_ES
dc.subject Regenerative medicine es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title Design and characterization of microspheres for a 3D mesenchymal stem cell culture es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.colsurfb.2020.111322 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UNLP//11%2FX768/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106099RB-C41/ES/MICROGELES BIOMIMETICOS PARA EL ESTUDIO DE LA GENERACION DE RESISTENCIAS A FARMACOS EN EL MIELOMA MULTIPLE./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2016-76039-C4-1-R/ES/BIOMATERIALES PIEZOELECTRICOS PARA LA DIFERENCIACION CELULAR EN INTERFASES CELULA-MATERIAL ELECTRICAMENTE ACTIVAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Lastra, ML.; Gómez Ribelles, JL.; Cortizo, AM. (2020). Design and characterization of microspheres for a 3D mesenchymal stem cell culture. Colloids and Surfaces B Biointerfaces. 196:1-8. https://doi.org/10.1016/j.colsurfb.2020.111322 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.colsurfb.2020.111322 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 8 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 196 es_ES
dc.identifier.pmid 32841788 es_ES
dc.relation.pasarela S\433286 es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Universidad Nacional de La Plata, Argentina es_ES
dc.contributor.funder Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina es_ES
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dc.subject.ods 03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades es_ES


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