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dc.contributor.author | Araque Monrós, María Carmen | es_ES |
dc.contributor.author | Gil-Santos, Luis | es_ES |
dc.contributor.author | Monleón Pradas, Manuel | es_ES |
dc.contributor.author | Más Estellés, Jorge | es_ES |
dc.date.accessioned | 2021-05-01T03:31:08Z | |
dc.date.available | 2021-05-01T03:31:08Z | |
dc.date.issued | 2020-10-02 | es_ES |
dc.identifier.issn | 1743-4440 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/165836 | |
dc.description.abstract | [EN] Objective: Although several different types of bioreactors are currently available with mechanical stimulation of constructs or prostheses for tendon regeneration, they are in many cases expensive and difficult to operate. This paper proposes a simple bioreactor to mechanically stimulate up to three constructs for tendon and ligament repair, composed of a stainless-steel frame and an electric motor. Methods: The deformation is produced by a cam wheel, whose eccentricity defines the maximum deformation. The test samples, braids of PLA seeded in surface with mouse fibroblasts, are immersed in the culture medium during mechanical stimulation. Results: Its advantages over existing similar bioreactor designs include: easy renewal of the culture medium and an external electric motor to avoid heating and contamination issues. After 14 days of stretching, the culture samples showed enhanced cellular proliferation and cell fiber alignment in addition to higher production of type I collagen. The cells initially seeded on the braid surface migrated to the inside of the braid. Conclusion: Although the results obtained have a poor statistical basis, they do suggest that the bioreactor could be usefully applied to stimulate constructs for tendon and ligament repair. Anyway, further experiments should be conducted in the future. | es_ES |
dc.description.sponsorship | This paper was funded through a researching contract with the Researching Association of the Textil Industries (AITEX, Alcoi, Spain). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Taylor & Francis | es_ES |
dc.relation.ispartof | Expert Review of Medical Devices | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Bioreactor | es_ES |
dc.subject | Mechanical stimulation | es_ES |
dc.subject | Construct | es_ES |
dc.subject | Regeneration | es_ES |
dc.subject | Tendon | es_ES |
dc.subject.classification | FISICA APLICADA | es_ES |
dc.subject.classification | MAQUINAS Y MOTORES TERMICOS | es_ES |
dc.title | New bioreactor for mechanical stimulation of cultured tendon-like constructs: design and validation. | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1080/17434440.2020.1825072 | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada | es_ES |
dc.description.bibliographicCitation | Araque Monrós, MC.; Gil-Santos, L.; Monleón Pradas, M.; Más Estellés, J. (2020). New bioreactor for mechanical stimulation of cultured tendon-like constructs: design and validation. Expert Review of Medical Devices. 17(10):1115-1121. https://doi.org/10.1080/17434440.2020.1825072 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1080/17434440.2020.1825072 | es_ES |
dc.description.upvformatpinicio | 1115 | es_ES |
dc.description.upvformatpfin | 1121 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 17 | es_ES |
dc.description.issue | 10 | es_ES |
dc.identifier.pmid | 32938254 | es_ES |
dc.relation.pasarela | S\423229 | es_ES |
dc.contributor.funder | Asociación de Investigación de la Industria Textil | es_ES |
dc.description.references | Murray, G. A. W., & Semple, J. C. (1979). A review of work on artificial tendons. Journal of Biomedical Engineering, 1(3), 177-184. doi:10.1016/0141-5425(79)90040-2 | es_ES |
dc.description.references | Ricci, J. L., Gona, A. G., Alexander, H., & Parsons, J. R. (1984). Morphological characteristics of tendon cells cultured on synthetic fibers. Journal of Biomedical Materials Research, 18(9), 1073-1087. doi:10.1002/jbm.820180910 | es_ES |
dc.description.references | HUNTER, J. M., & SALISBURY, R. E. (1971). Flexor-Tendon Reconstruction in Severely Damaged Hands. The Journal of Bone & Joint Surgery, 53(5), 829-858. doi:10.2106/00004623-197153050-00001 | es_ES |
dc.description.references | Hunter, J. M., Singer, D. I., Jaeger, S. H., & Mackin, E. J. (1988). Active tendon implants in flexor tendon reconstruction. The Journal of Hand Surgery, 13(6), 849-859. doi:10.1016/0363-5023(88)90259-6 | es_ES |
dc.description.references | Walden, G., Liao, X., Donell, S., Raxworthy, M. J., Riley, G. P., & Saeed, A. (2017). A Clinical, Biological, and Biomaterials Perspective into Tendon Injuries and Regeneration. Tissue Engineering Part B: Reviews, 23(1), 44-58. doi:10.1089/ten.teb.2016.0181 | es_ES |
dc.description.references | Araque Monrós C, Gil Santos L, Gironés Bernabé S, et al. Universitat Politècnica de València. Procedimiento de obtención de una prótesis biodegradable. Patent of invention nº P201130919. 2011. | es_ES |
dc.description.references | Freeman, J. W., Woods, M. D., & Laurencin, C. T. (2007). Tissue engineering of the anterior cruciate ligament using a braid–twist scaffold design. Journal of Biomechanics, 40(9), 2029-2036. doi:10.1016/j.jbiomech.2006.09.025 | es_ES |
dc.description.references | Laurencin, C. T., & Freeman, J. W. (2005). Ligament tissue engineering: An evolutionary materials science approach. Biomaterials, 26(36), 7530-7536. doi:10.1016/j.biomaterials.2005.05.073 | es_ES |
dc.description.references | Merolli, A., & Joyce, T. J. (Eds.). (2009). Biomaterials in Hand Surgery. doi:10.1007/978-88-470-1195-3 | es_ES |
dc.description.references | Moreau, J. E., Bramono, D. S., Horan, R. L., Kaplan, D. L., & Altman, G. H. (2008). Sequential Biochemical and Mechanical Stimulation in the Development of Tissue-Engineered Ligaments. Tissue Engineering Part A, 14(7), 1161-1172. doi:10.1089/ten.tea.2007.0147 | es_ES |
dc.description.references | Nirmalanandhan, V. S., Rao, M., Shearn, J. T., Juncosa-Melvin, N., Gooch, C., & Butler, D. L. (2008). Effect of scaffold material, construct length and mechanical stimulation on the in vitro stiffness of the engineered tendon construct. Journal of Biomechanics, 41(4), 822-828. doi:10.1016/j.jbiomech.2007.11.009 | es_ES |
dc.description.references | Sumanasinghe, R. D., Osborne, J. A., & Loboa, E. G. (2008). Mesenchymal stem cell‐seeded collagen matrices for bone repair: Effects of cyclic tensile strain, cell density, and media conditions on matrix contraction in vitro. Journal of Biomedical Materials Research Part A, 88A(3), 778-786. doi:10.1002/jbm.a.31913 | es_ES |
dc.description.references | Saber, S., Zhang, A. Y., Ki, S. H., Lindsey, D. P., Smith, R. L., Riboh, J., … Chang, J. (2010). Flexor Tendon Tissue Engineering: Bioreactor Cyclic Strain Increases Construct Strength. Tissue Engineering Part A, 16(6), 2085-2090. doi:10.1089/ten.tea.2010.0032 | es_ES |
dc.description.references | Tohyama, H., & Yasuda, K. (2000). The effects of stress enhancement on the extracellular matrix and fibroblasts in the patellar tendon. Journal of Biomechanics, 33(5), 559-565. doi:10.1016/s0021-9290(99)00217-1 | es_ES |
dc.description.references | Wang, T., Lin, Z., Day, R. E., Gardiner, B., Landao-Bassonga, E., Rubenson, J., … Zheng, M. H. (2013). Programmable mechanical stimulation influences tendon homeostasis in a bioreactor system. Biotechnology and Bioengineering, 110(5), 1495-1507. doi:10.1002/bit.24809 | es_ES |
dc.description.references | Wang, T., Gardiner, B. S., Lin, Z., Rubenson, J., Kirk, T. B., Wang, A., … Zheng, M. H. (2013). Bioreactor Design for Tendon/Ligament Engineering. Tissue Engineering Part B: Reviews, 19(2), 133-146. doi:10.1089/ten.teb.2012.0295 | es_ES |
dc.description.references | Abousleiman, R. I., Reyes, Y., McFetridge, P., & Sikavitsas, V. (2009). Tendon Tissue Engineering Using Cell-Seeded Umbilical Veins Cultured in a Mechanical Stimulator. Tissue Engineering Part A, 15(4), 787-795. doi:10.1089/ten.tea.2008.0102 | es_ES |
dc.description.references | Masuda, T., Takahashi, I., Anada, T., Arai, F., Fukuda, T., Takano-Yamamoto, T., & Suzuki, O. (2008). Development of a cell culture system loading cyclic mechanical strain to chondrogenic cells. Journal of Biotechnology, 133(2), 231-238. doi:10.1016/j.jbiotec.2007.08.007 | es_ES |
dc.description.references | Xu, Z. C., Zhang, W. J., Li, H., Cui, L., Cen, L., Zhou, G. D., … Cao, Y. (2008). Engineering of an elastic large muscular vessel wall with pulsatile stimulation in bioreactor. Biomaterials, 29(10), 1464-1472. doi:10.1016/j.biomaterials.2007.11.037 | es_ES |
dc.description.references | TC-3F Ebers Medical Technology, S.L. [cited 2019 May 15]. Available from: https://ebersmedical.com/tissue-engineering/bioreactors/load-culture/tc-3f-bioreactor. | es_ES |
dc.description.references | CellScale biomaterials testing. [cited 2020 Mar 16]. Available from: https://cellscale.com/https://www.cellscale.com/products/mct6 | es_ES |
dc.description.references | Lim, W. L., Liau, L. L., Ng, M. H., Chowdhury, S. R., & Law, J. X. (2019). Current Progress in Tendon and Ligament Tissue Engineering. Tissue Engineering and Regenerative Medicine, 16(6), 549-571. doi:10.1007/s13770-019-00196-w | es_ES |
dc.description.references | Oftadeh, R., Connizzo, B. K., Nia, H. T., Ortiz, C., & Grodzinsky, A. J. (2018). Biological connective tissues exhibit viscoelastic and poroelastic behavior at different frequency regimes: Application to tendon and skin biophysics. Acta Biomaterialia, 70, 249-259. doi:10.1016/j.actbio.2018.01.041 | es_ES |
dc.description.references | Vashaghian, M., Diedrich, C. M., Zandieh-Doulabi, B., Werner, A., Smit, T. H., & Roovers, J. P. (2019). Gentle cyclic straining of human fibroblasts on electrospun scaffolds enhances their regenerative potential. Acta Biomaterialia, 84, 159-168. doi:10.1016/j.actbio.2018.11.034 | es_ES |
dc.description.references | Helms, F., Lau, S., Klingenberg, M., Aper, T., Haverich, A., Wilhelmi, M., & Böer, U. (2019). Complete Myogenic Differentiation of Adipogenic Stem Cells Requires Both Biochemical and Mechanical Stimulation. Annals of Biomedical Engineering, 48(3), 913-926. doi:10.1007/s10439-019-02234-z | es_ES |
dc.description.references | Araque-Monrós, M. C., García-Cruz, D. M., Escobar-Ivirico, J. L., Gil-Santos, L., Monleón-Pradas, M., & Más-Estellés, J. (2019). Regenerative and Resorbable PLA/HA Hybrid Construct for Tendon/Ligament Tissue Engineering. Annals of Biomedical Engineering, 48(2), 757-767. doi:10.1007/s10439-019-02403-0 | es_ES |
dc.description.references | Yang, G., Crawford, R. C., & Wang, J. H.-C. (2004). Proliferation and collagen production of human patellar tendon fibroblasts in response to cyclic uniaxial stretching in serum-free conditions. Journal of Biomechanics, 37(10), 1543-1550. doi:10.1016/j.jbiomech.2004.01.005 | es_ES |
dc.description.references | Surrao, D. C., Fan, J. C. Y., Waldman, S. D., & Amsden, B. G. (2012). A crimp-like microarchitecture improves tissue production in fibrous ligament scaffolds in response to mechanical stimuli. Acta Biomaterialia, 8(10), 3704-3713. doi:10.1016/j.actbio.2012.06.016 | es_ES |
dc.description.references | Wang, J. H.-C. (2006). Mechanobiology of tendon. Journal of Biomechanics, 39(9), 1563-1582. doi:10.1016/j.jbiomech.2005.05.011 | es_ES |
dc.description.references | Zhang, C., Zhu, J., Zhou, Y., Thampatty, B. P., & Wang, J. H.-C. (2019). Tendon Stem/Progenitor Cells and Their Interactions with Extracellular Matrix and Mechanical Loading. Stem Cells International, 2019, 1-10. doi:10.1155/2019/3674647 | es_ES |