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New bioreactor for mechanical stimulation of cultured tendon-like constructs: design and validation.

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New bioreactor for mechanical stimulation of cultured tendon-like constructs: design and validation.

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dc.contributor.author Araque Monrós, María Carmen es_ES
dc.contributor.author Gil-Santos, Luis es_ES
dc.contributor.author Monleón Pradas, Manuel es_ES
dc.contributor.author Más Estellés, Jorge es_ES
dc.date.accessioned 2021-05-01T03:31:08Z
dc.date.available 2021-05-01T03:31:08Z
dc.date.issued 2020-10-02 es_ES
dc.identifier.issn 1743-4440 es_ES
dc.identifier.uri http://hdl.handle.net/10251/165836
dc.description.abstract [EN] Objective: Although several different types of bioreactors are currently available with mechanical stimulation of constructs or prostheses for tendon regeneration, they are in many cases expensive and difficult to operate. This paper proposes a simple bioreactor to mechanically stimulate up to three constructs for tendon and ligament repair, composed of a stainless-steel frame and an electric motor. Methods: The deformation is produced by a cam wheel, whose eccentricity defines the maximum deformation. The test samples, braids of PLA seeded in surface with mouse fibroblasts, are immersed in the culture medium during mechanical stimulation. Results: Its advantages over existing similar bioreactor designs include: easy renewal of the culture medium and an external electric motor to avoid heating and contamination issues. After 14 days of stretching, the culture samples showed enhanced cellular proliferation and cell fiber alignment in addition to higher production of type I collagen. The cells initially seeded on the braid surface migrated to the inside of the braid. Conclusion: Although the results obtained have a poor statistical basis, they do suggest that the bioreactor could be usefully applied to stimulate constructs for tendon and ligament repair. Anyway, further experiments should be conducted in the future. es_ES
dc.description.sponsorship This paper was funded through a researching contract with the Researching Association of the Textil Industries (AITEX, Alcoi, Spain). es_ES
dc.language Inglés es_ES
dc.publisher Taylor & Francis es_ES
dc.relation.ispartof Expert Review of Medical Devices es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Bioreactor es_ES
dc.subject Mechanical stimulation es_ES
dc.subject Construct es_ES
dc.subject Regeneration es_ES
dc.subject Tendon es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title New bioreactor for mechanical stimulation of cultured tendon-like constructs: design and validation. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1080/17434440.2020.1825072 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.description.bibliographicCitation Araque Monrós, MC.; Gil-Santos, L.; Monleón Pradas, M.; Más Estellés, J. (2020). New bioreactor for mechanical stimulation of cultured tendon-like constructs: design and validation. Expert Review of Medical Devices. 17(10):1115-1121. https://doi.org/10.1080/17434440.2020.1825072 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1080/17434440.2020.1825072 es_ES
dc.description.upvformatpinicio 1115 es_ES
dc.description.upvformatpfin 1121 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 17 es_ES
dc.description.issue 10 es_ES
dc.identifier.pmid 32938254 es_ES
dc.relation.pasarela S\423229 es_ES
dc.contributor.funder Asociación de Investigación de la Industria Textil es_ES
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