Mostrar el registro sencillo del ítem
dc.contributor.author | Muhammad, Muhammad | es_ES |
dc.contributor.author | Willems, Christian | es_ES |
dc.contributor.author | Rodríguez-Fernández, Julio | es_ES |
dc.contributor.author | Gallego Ferrer, Gloria | es_ES |
dc.contributor.author | Groth, Thomas | es_ES |
dc.date.accessioned | 2021-05-01T03:31:13Z | |
dc.date.available | 2021-05-01T03:31:13Z | |
dc.date.issued | 2020-08 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/165837 | |
dc.description.abstract | [EN] Polysaccharides are widely used as building blocks of scaffolds and hydrogels in tissue engineering, which may require their chemical modification to permit crosslinking. The goal of this study was to generate a library of oxidized alginate (oALG) and oxidized hyaluronic acid (oHA) that can be used for in situ gelling hydrogels by covalent reaction between aldehyde groups of the oxidized polysaccharides (oPS) and amino groups of carboxymethyl chitosan (CMC) through imine bond formation. Here, we studied the effect of sodium periodate concentration and reaction time on aldehyde content, molecular weight of derivatives and cytotoxicity of oPS towards 3T3-L1 fibroblasts. It was found that the molecular weights of all oPs decreased with oxidation and that the degree of oxidation was generally higher in oHA than in oALG. Studies showed that only oPs with an oxidation degree above 25% were cytotoxic. Initial studies were also done on the crosslinking of oPs with CMC showing with rheometry that rather soft gels were formed from higher oxidized oPs possessing a moderate cytotoxicity. The results of this study indicate the potential of oALG and oHA for use as in situ gelling hydrogels or inks in bioprinting for application in tissue engineering and controlled release. | es_ES |
dc.description.sponsorship | This work was supported by the Deutscher Akademischer Austauschdienst DAAD (grant No. 91605199 to MM) and Deutsche Forschungsgemeinschaft (grant Gr1290/11-1 to TG). The kind support by Spanish State Research Agency (AEI) through the PID2019-106000RB-C21/AEI/10.13039/501100011033 project (including the FEDER financial support) to GGF is acknowledged. We acknowledge the financial support within the funding programme "Open Access Publishing" by the German Research Foundation (DFG).We are very thankful to Andrea Liedmann for her guidance during the cell experiments and Alexandros Repanas for his help during the synthesis and characterization of oPs and data analyses. Furthermore, Marie-Luise Trutschel is acknowledged for her guidance during the rheological measurements. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI AG | es_ES |
dc.relation.ispartof | Biomolecules | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Alginate | es_ES |
dc.subject | Hyaluronic acid | es_ES |
dc.subject | Oxidation | es_ES |
dc.subject | In situ gelling | es_ES |
dc.subject | Hydrogels | es_ES |
dc.subject | Fibroblasts | es_ES |
dc.subject | Cytotoxicity | es_ES |
dc.subject.classification | MAQUINAS Y MOTORES TERMICOS | es_ES |
dc.title | Synthesis and Characterization of Oxidized Polysaccharides for In Situ Forming Hydrogels | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/biom10081185 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/DAAD//91605199/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/DFG//Gr1290%2F11-1/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106000RB-C21/ES/HIDROGELES BIOMIMETICOS IMPRIMIBLES CON PRESENTACION DE FACTORES DE CRECIMIENTO EFICIENTE PARA ESTUDIOS DE HEPATOTOXICIDAD DE ALTO RENDIMIENTO/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada | es_ES |
dc.description.bibliographicCitation | Muhammad, M.; Willems, C.; Rodríguez-Fernández, J.; Gallego Ferrer, G.; Groth, T. (2020). Synthesis and Characterization of Oxidized Polysaccharides for In Situ Forming Hydrogels. Biomolecules. 10(8):1-18. https://doi.org/10.3390/biom10081185 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/biom10081185 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 18 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 10 | es_ES |
dc.description.issue | 8 | es_ES |
dc.identifier.eissn | 2218-273X | es_ES |
dc.identifier.pmid | 32824101 | es_ES |
dc.identifier.pmcid | PMC7464976 | es_ES |
dc.relation.pasarela | S\430793 | es_ES |
dc.contributor.funder | Deutsche Forschungsgemeinschaft | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Deutscher Akademischer Austauschdienst | es_ES |
dc.description.references | Ratner, B. D. (2019). Biomaterials: Been There, Done That, and Evolving into the Future. Annual Review of Biomedical Engineering, 21(1), 171-191. doi:10.1146/annurev-bioeng-062117-120940 | es_ES |
dc.description.references | Morais, J. M., Papadimitrakopoulos, F., & Burgess, D. J. (2010). Biomaterials/Tissue Interactions: Possible Solutions to Overcome Foreign Body Response. The AAPS Journal, 12(2), 188-196. doi:10.1208/s12248-010-9175-3 | es_ES |
dc.description.references | Domingues, R. M. A., Silva, M., Gershovich, P., Betta, S., Babo, P., Caridade, S. G., … Gomes, M. E. (2015). Development of Injectable Hyaluronic Acid/Cellulose Nanocrystals Bionanocomposite Hydrogels for Tissue Engineering Applications. Bioconjugate Chemistry, 26(8), 1571-1581. doi:10.1021/acs.bioconjchem.5b00209 | es_ES |
dc.description.references | Pop-Georgievski, O., Zimmermann, R., Kotelnikov, I., Proks, V., Romeis, D., Kučka, J., … Werner, C. (2018). Impact of Bioactive Peptide Motifs on Molecular Structure, Charging, and Nonfouling Properties of Poly(ethylene oxide) Brushes. Langmuir, 34(21), 6010-6020. doi:10.1021/acs.langmuir.8b00441 | es_ES |
dc.description.references | Wen, Q., Mithieux, S. M., & Weiss, A. S. (2020). Elastin Biomaterials in Dermal Repair. Trends in Biotechnology, 38(3), 280-291. doi:10.1016/j.tibtech.2019.08.005 | es_ES |
dc.description.references | Trujillo, S., Gonzalez-Garcia, C., Rico, P., Reid, A., Windmill, J., Dalby, M. J., & Salmeron-Sanchez, M. (2020). Engineered 3D hydrogels with full-length fibronectin that sequester and present growth factors. Biomaterials, 252, 120104. doi:10.1016/j.biomaterials.2020.120104 | es_ES |
dc.description.references | Xu, M., Pradhan, S., Agostinacchio, F., Pal, R. K., Greco, G., Mazzolai, B., … Yadavalli, V. K. (2019). Easy, Scalable, Robust, Micropatterned Silk Fibroin Cell Substrates. Advanced Materials Interfaces, 6(8), 1801822. doi:10.1002/admi.201801822 | es_ES |
dc.description.references | Köwitsch, A., Zhou, G., & Groth, T. (2017). Medical application of glycosaminoglycans: a review. Journal of Tissue Engineering and Regenerative Medicine, 12(1), e23-e41. doi:10.1002/term.2398 | es_ES |
dc.description.references | Yang, Y., Lu, Y., Zeng, K., Heinze, T., Groth, T., & Zhang, K. (2020). Recent Progress on Cellulose‐Based Ionic Compounds for Biomaterials. Advanced Materials, 33(28), 2000717. doi:10.1002/adma.202000717 | es_ES |
dc.description.references | Yu, Y., Shen, M., Song, Q., & Xie, J. (2018). Biological activities and pharmaceutical applications of polysaccharide from natural resources: A review. Carbohydrate Polymers, 183, 91-101. doi:10.1016/j.carbpol.2017.12.009 | es_ES |
dc.description.references | Grasdalen, H. (1983). High-field, 1H-n.m.r. spectroscopy of alginate: sequential structure and linkage conformations. Carbohydrate Research, 118, 255-260. doi:10.1016/0008-6215(83)88053-7 | es_ES |
dc.description.references | Criado-Gonzalez, M., Fernandez-Gutierrez, M., San Roman, J., Mijangos, C., & Hernández, R. (2019). Local and controlled release of tamoxifen from multi (layer-by-layer) alginate/chitosan complex systems. Carbohydrate Polymers, 206, 428-434. doi:10.1016/j.carbpol.2018.11.007 | es_ES |
dc.description.references | Kirdponpattara, S., Khamkeaw, A., Sanchavanakit, N., Pavasant, P., & Phisalaphong, M. (2015). Structural modification and characterization of bacterial cellulose–alginate composite scaffolds for tissue engineering. Carbohydrate Polymers, 132, 146-155. doi:10.1016/j.carbpol.2015.06.059 | es_ES |
dc.description.references | Price, R. D., Berry, M. G., & Navsaria, H. A. (2007). Hyaluronic acid: the scientific and clinical evidence. Journal of Plastic, Reconstructive & Aesthetic Surgery, 60(10), 1110-1119. doi:10.1016/j.bjps.2007.03.005 | es_ES |
dc.description.references | Kristiansen, K. A., Potthast, A., & Christensen, B. E. (2010). Periodate oxidation of polysaccharides for modification of chemical and physical properties. Carbohydrate Research, 345(10), 1264-1271. doi:10.1016/j.carres.2010.02.011 | es_ES |
dc.description.references | Millan, C., Cavalli, E., Groth, T., Maniura-Weber, K., & Zenobi-Wong, M. (2015). Engineered Microtissues Formed by Schiff Base Crosslinking Restore the Chondrogenic Potential of Aged Mesenchymal Stem Cells. Advanced Healthcare Materials, 4(9), 1348-1358. doi:10.1002/adhm.201500102 | es_ES |
dc.description.references | Reyes, J. M. G., Herretes, S., Pirouzmanesh, A., Wang, D.-A., Elisseeff, J. H., Jun, A., … Behrens, A. (2005). A Modified Chondroitin Sulfate Aldehyde Adhesive for Sealing Corneal Incisions. Investigative Opthalmology & Visual Science, 46(4), 1247. doi:10.1167/iovs.04-1192 | es_ES |
dc.description.references | Peppas, N. A., Hilt, J. Z., Khademhosseini, A., & Langer, R. (2006). Hydrogels in Biology and Medicine: From Molecular Principles to Bionanotechnology. Advanced Materials, 18(11), 1345-1360. doi:10.1002/adma.200501612 | es_ES |
dc.description.references | Van Tomme, S. R., Storm, G., & Hennink, W. E. (2008). In situ gelling hydrogels for pharmaceutical and biomedical applications. International Journal of Pharmaceutics, 355(1-2), 1-18. doi:10.1016/j.ijpharm.2008.01.057 | es_ES |
dc.description.references | Mota, C., Camarero-Espinosa, S., Baker, M. B., Wieringa, P., & Moroni, L. (2020). Bioprinting: From Tissue and Organ Development to in Vitro Models. Chemical Reviews, 120(19), 10547-10607. doi:10.1021/acs.chemrev.9b00789 | es_ES |
dc.description.references | Matyash, M., Despang, F., Ikonomidou, C., & Gelinsky, M. (2014). Swelling and Mechanical Properties of Alginate Hydrogels with Respect to Promotion of Neural Growth. Tissue Engineering Part C: Methods, 20(5), 401-411. doi:10.1089/ten.tec.2013.0252 | es_ES |
dc.description.references | Berger, J., Reist, M., Mayer, J. M., Felt, O., Peppas, N. A., & Gurny, R. (2004). Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications. European Journal of Pharmaceutics and Biopharmaceutics, 57(1), 19-34. doi:10.1016/s0939-6411(03)00161-9 | es_ES |
dc.description.references | Segura, T., Anderson, B. C., Chung, P. H., Webber, R. E., Shull, K. R., & Shea, L. D. (2005). Crosslinked hyaluronic acid hydrogels: a strategy to functionalize and pattern. Biomaterials, 26(4), 359-371. doi:10.1016/j.biomaterials.2004.02.067 | es_ES |
dc.description.references | De la Riva, B., Nowak, C., Sánchez, E., Hernández, A., Schulz-Siegmund, M., Pec, M. K., … Évora, C. (2009). VEGF-controlled release within a bone defect from alginate/chitosan/PLA-H scaffolds. European Journal of Pharmaceutics and Biopharmaceutics, 73(1), 50-58. doi:10.1016/j.ejpb.2009.04.014 | es_ES |
dc.description.references | Yang, Y., Köwitsch, A., Ma, N., Mäder, K., Pashkuleva, I., Reis, R. L., & Groth, T. (2015). Functionality of surface-coupled oxidised glycosaminoglycans towards fibroblast adhesion. Journal of Bioactive and Compatible Polymers, 31(2), 191-207. doi:10.1177/0883911515599999 | es_ES |
dc.description.references | Köwitsch, A., Yang, Y., Ma, N., Kuntsche, J., Mäder, K., & Groth, T. (2011). Bioactivity of immobilized hyaluronic acid derivatives regarding protein adsorption and cell adhesion. Biotechnology and Applied Biochemistry, 58(5), 376-389. doi:10.1002/bab.41 | es_ES |
dc.description.references | Korzhikov, V., Roeker, S., Vlakh, E., Kasper, C., & Tennikova, T. (2008). Synthesis of Multifunctional Polyvinylsaccharide Containing Controllable Amounts of Biospecific Ligands. Bioconjugate Chemistry, 19(3), 617-625. doi:10.1021/bc700383w | es_ES |
dc.description.references | Zhao, M., Li, L., Zhou, C., Heyroth, F., Fuhrmann, B., Maeder, K., & Groth, T. (2014). Improved Stability and Cell Response by Intrinsic Cross-Linking of Multilayers from Collagen I and Oxidized Glycosaminoglycans. Biomacromolecules, 15(11), 4272-4280. doi:10.1021/bm501286f | es_ES |
dc.description.references | Tang, Q.-Q., Otto, T. C., & Lane, M. D. (2004). Commitment of C3H10T1/2 pluripotent stem cells to the adipocyte lineage. Proceedings of the National Academy of Sciences, 101(26), 9607-9611. doi:10.1073/pnas.0403100101 | es_ES |
dc.description.references | Alarake, N. Z., Frohberg, P., Groth, T., & Pietzsch, M. (2017). Mechanical Properties and Biocompatibility of in Situ Enzymatically Cross-Linked Gelatin Hydrogels. The International Journal of Artificial Organs, 40(4), 159-168. doi:10.5301/ijao.5000553 | es_ES |
dc.description.references | Morra, M. (2005). Engineering of Biomaterials Surfaces by Hyaluronan. Biomacromolecules, 6(3), 1205-1223. doi:10.1021/bm049346i | es_ES |
dc.description.references | Zhang, R., Xue, M., Yang, J., & Tan, T. (2011). A novel injectable and in situ crosslinked hydrogel based on hyaluronic acid and α,β-polyaspartylhydrazide. Journal of Applied Polymer Science, 125(2), 1116-1126. doi:10.1002/app.34828 | es_ES |
dc.description.references | Jejurikar, A., Seow, X. T., Lawrie, G., Martin, D., Jayakrishnan, A., & Grøndahl, L. (2012). Degradable alginate hydrogels crosslinked by the macromolecular crosslinker alginate dialdehyde. Journal of Materials Chemistry, 22(19), 9751. doi:10.1039/c2jm30564j | es_ES |
dc.description.references | Emami, Z., Ehsani, M., Zandi, M., & Foudazi, R. (2018). Controlling alginate oxidation conditions for making alginate-gelatin hydrogels. Carbohydrate Polymers, 198, 509-517. doi:10.1016/j.carbpol.2018.06.080 | es_ES |
dc.description.references | Yegappan, R., Selvaprithiviraj, V., Mohandas, A., & Jayakumar, R. (2019). Nano polydopamine crosslinked thiol-functionalized hyaluronic acid hydrogel for angiogenic drug delivery. Colloids and Surfaces B: Biointerfaces, 177, 41-49. doi:10.1016/j.colsurfb.2019.01.035 | es_ES |
dc.description.references | Bouhadir, K. H., Lee, K. Y., Alsberg, E., Damm, K. L., Anderson, K. W., & Mooney, D. J. (2001). Degradation of Partially Oxidized Alginate and Its Potential Application for Tissue Engineering. Biotechnology Progress, 17(5), 945-950. doi:10.1021/bp010070p | es_ES |
dc.description.references | Strätz, J., Liedmann, A., Heinze, T., Fischer, S., & Groth, T. (2019). Effect of Sulfation Route and Subsequent Oxidation on Derivatization Degree and Biocompatibility of Cellulose Sulfates. Macromolecular Bioscience, 20(2), 1900403. doi:10.1002/mabi.201900403 | es_ES |
dc.description.references | Elahipanah, S., O’Brien, P. J., Rogozhnikov, D., & Yousaf, M. N. (2017). General Dialdehyde Click Chemistry for Amine Bioconjugation. Bioconjugate Chemistry, 28(5), 1422-1433. doi:10.1021/acs.bioconjchem.7b00106 | es_ES |
dc.description.references | Huang, G., & Huang, H. (2018). Application of hyaluronic acid as carriers in drug delivery. Drug Delivery, 25(1), 766-772. doi:10.1080/10717544.2018.1450910 | es_ES |
dc.description.references | Qhattal, H. S. S., & Liu, X. (2011). Characterization of CD44-Mediated Cancer Cell Uptake and Intracellular Distribution of Hyaluronan-Grafted Liposomes. Molecular Pharmaceutics, 8(4), 1233-1246. doi:10.1021/mp2000428 | es_ES |
dc.description.references | Andersen, T., Auk-Emblem, P., & Dornish, M. (2015). 3D Cell Culture in Alginate Hydrogels. Microarrays, 4(2), 133-161. doi:10.3390/microarrays4020133 | es_ES |
dc.description.references | Poveda-Reyes, S., Moulisova, V., Sanmartín-Masiá, E., Quintanilla-Sierra, L., Salmerón-Sánchez, M., & Ferrer, G. G. (2016). Gelatin-Hyaluronic Acid Hydrogels with Tuned Stiffness to Counterbalance Cellular Forces and Promote Cell Differentiation. Macromolecular Bioscience, 16(9), 1311-1324. doi:10.1002/mabi.201500469 | es_ES |
dc.description.references | Poveda-Reyes, S., Rodrigo-Navarro, A., Gamboa-Martínez, T. C., Rodíguez-Cabello, J. C., Quintanilla-Sierra, L., Edlund, U., & Ferrer, G. G. (2015). Injectable composites of loose microfibers and gelatin with improved interfacial interaction for soft tissue engineering. Polymer, 74, 224-234. doi:10.1016/j.polymer.2015.08.018 | es_ES |