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Borax induces osteogenesis by stimulating NaBC1 transporter via activation of BMP pathway

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Borax induces osteogenesis by stimulating NaBC1 transporter via activation of BMP pathway

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dc.contributor.author Rico Tortosa, Patricia María es_ES
dc.contributor.author Rodrigo Navarro, Aleixandre es_ES
dc.contributor.author Sánchez-Pérez, Laura es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.date.accessioned 2021-05-01T03:31:18Z
dc.date.available 2021-05-01T03:31:18Z
dc.date.issued 2020-11-27 es_ES
dc.identifier.uri http://hdl.handle.net/10251/165839
dc.description.abstract [EN] The intrinsic properties of mesenchymal stem cells (MSCs) make them ideal candidates for tissue engineering applications. Efforts have been made to control MSC behavior by using material systems to engineer synthetic extracellular matrices and/or include soluble factors in the media. This work proposes a simple approach based on ion transporter stimulation to determine stem cell fate that avoids the use of growth factors. Addition of borax alone, transported by the NaBC1-transporter, enhanced MSC adhesion and contractility, promoted osteogenesis and inhibited adipogenesis. Stimulated-NaBC1 promoted osteogenesis via the BMP canonical pathway (comprising Smad1/YAP nucleus translocation and osteopontin expression) through a mechanism that involves simultaneous NaBC1/BMPR1A and NaBC1/alpha (5)beta (1)/alpha (v)beta (3) co-localization. We describe an original function for NaBC1 transporter, besides controlling borate homeostasis, capable of stimulating growth factor receptors and fibronectin-binding integrins. Our results open up new biomaterial engineering approaches for biomedical applications by a cost-effective strategy that avoids the use of soluble growth factors. Rico et al. propose a simple approach based on borax stimulation of NaBC1 transporter, which enhances FN-binding integrin-dependent mesenchymal stem cell adhesion and contractility, promotes osteogenesis and inhibits adipogenesis. Osteogenic differentiation depends on activation of the BMP pathway through a mechanism that involves simultaneous co-localization of NaBC1 with FN-binding integrins and BMPR1A. es_ES
dc.description.sponsorship P.R. acknowledges support from the Spanish Ministry of Science, Innovation and Universities (RTI2018-096794), and Fondo Europeo de Desarrollo Regional (FEDER). CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. M.S.S. acknowledges support from the UK Engineering and Physical Sciences Research Council (EPSRC-EP/P001114/1). es_ES
dc.language Inglés es_ES
dc.publisher Springer Nature es_ES
dc.relation.ispartof Communications Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Borax es_ES
dc.subject NaBC1 es_ES
dc.subject Integrins es_ES
dc.subject Fibronectin es_ES
dc.subject Mesenchymal Stem Cells es_ES
dc.subject Osteogenesis es_ES
dc.subject Cell repceptor crosstalk es_ES
dc.subject Intracellular tension es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Borax induces osteogenesis by stimulating NaBC1 transporter via activation of BMP pathway es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/s42003-020-01449-4 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UKRI//EP%2FP001114%2F1/GB/Engineering growth factor microenvironments - a new therapeutic paradigm for regenerative medicine/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096794-B-I00/ES/DISEÑO DE MICROENTORNOS CELULARES PARA PROMOVER LA MECANOTRANSDUCCION SINERGICA DE CANALES DE IONES E INTEGRINAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.description.bibliographicCitation Rico Tortosa, PM.; Rodrigo Navarro, A.; Sánchez-Pérez, L.; Salmerón Sánchez, M. (2020). Borax induces osteogenesis by stimulating NaBC1 transporter via activation of BMP pathway. Communications Biology. 3(1):1-15. https://doi.org/10.1038/s42003-020-01449-4 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/s42003-020-01449-4 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 15 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 3 es_ES
dc.description.issue 1 es_ES
dc.identifier.eissn 2399-3642 es_ES
dc.identifier.pmid 33247189 es_ES
dc.identifier.pmcid PMC7695834 es_ES
dc.relation.pasarela S\434893 es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
dc.contributor.funder UK Research and Innovation es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Engineering and Physical Sciences Research Council, Reino Unido es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina es_ES
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