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Ranolazine-mediated attenuation of mechanoelectric feedback in atrial myocyte monolayers

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Ranolazine-mediated attenuation of mechanoelectric feedback in atrial myocyte monolayers

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Nombre: Del-Canto;Gómez-C ...
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dc.contributor.author Del-Canto, Irene es_ES
dc.contributor.author Gómez-Cid, Lidia es_ES
dc.contributor.author Hernández-Romero, Ismael es_ES
dc.contributor.author Guillem Sánchez, María Salud es_ES
dc.contributor.author Fernández-Santos, María Eugenia es_ES
dc.contributor.author Atienza, Felipe es_ES
dc.contributor.author Such, Luis es_ES
dc.contributor.author Fernández-Avilés, Francisco es_ES
dc.contributor.author Chorro, Francisco J. es_ES
dc.contributor.author Martínez Climent, Batiste Andreu es_ES
dc.date.accessioned 2021-05-07T03:30:55Z
dc.date.available 2021-05-07T03:30:55Z
dc.date.issued 2020-08-04 es_ES
dc.identifier.issn 1664-042X es_ES
dc.identifier.uri http://hdl.handle.net/10251/166054
dc.description.abstract [EN] Background Mechanical stretch increases Na(+)inflow into myocytes, related to mechanisms including stretch-activated channels or Na+/H(+)exchanger activation, involving Ca(2+)increase that leads to changes in electrophysiological properties favoring arrhythmia induction. Ranolazine is an antianginal drug with confirmed beneficial effects against cardiac arrhythmias associated with the augmentation ofI(NaL)current and Ca(2+)overload. Objective This study investigates the effects of mechanical stretch on activation patterns in atrial cell monolayers and its pharmacological response to ranolazine. Methods Confluent HL-1 cells were cultured in silicone membrane plates and were stretched to 110% of original length. The characteristics ofin vitrofibrillation (dominant frequency, regularity index, density of phase singularities, rotor meandering, and rotor curvature) were analyzed using optical mapping in order to study the mechanoelectric response to stretch under control conditions and ranolazine action. Results HL-1 cell stretch increased fibrillatory dominant frequency (3.65 +/- 0.69 vs. 4.35 +/- 0.74 Hz,p< 0.01) and activation complexity (1.97 +/- 0.45 vs. 2.66 +/- 0.58 PS/cm(2),p< 0.01) under control conditions. These effects were related to stretch-induced changes affecting the reentrant patterns, comprising a decrease in rotor meandering (0.72 +/- 0.12 vs. 0.62 +/- 0.12 cm/s,p< 0.001) and an increase in wavefront curvature (4.90 +/- 0.42 vs. 5.68 +/- 0.40 rad/cm,p< 0.001). Ranolazine reduced stretch-induced effects, attenuating the activation rate increment (12.8% vs. 19.7%,p< 0.01) and maintaining activation complexity-both parameters being lower during stretch than under control conditions. Moreover, under baseline conditions, ranolazine slowed and regularized the activation patterns (3.04 +/- 0.61 vs. 3.65 +/- 0.69 Hz,p< 0.01). Conclusion Ranolazine attenuates the modifications of activation patterns induced by mechanical stretch in atrial myocyte monolayers. es_ES
dc.description.sponsorship This work was supported by the Instituto de Salud Carlos III-FEDER (Fondo Europeo de Desarrollo Regional) (Grant Nos. CB16/11/00486, CB16/11/00292, PI16/01123, PI17/01059, PI17/01106, PI18/01620, and DTS16/0160) and the Generalitat Valenciana (Grant Nos. PROMETEO/2018/078 and APOSTD/2018/181). es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation.ispartof Frontiers in Physiology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Mechanical stretch es_ES
dc.subject Mechanoelectric feedback es_ES
dc.subject Fibrillatory patterns es_ES
dc.subject Ranolazine es_ES
dc.subject Optical mapping es_ES
dc.subject Rotor dynamic analysis es_ES
dc.subject HL-1 cell es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title Ranolazine-mediated attenuation of mechanoelectric feedback in atrial myocyte monolayers es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fphys.2020.00922 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2018%2F078/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//PI16%2F01123/ES/Regeneración Cardiaca de Infarto Crónico Porcino mediante Inyecciónes Intramiocardiacas de Células Progenitoras Embebidas en Hidrogeles de Matriz Decelularizada/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ISCIII//PI17%2F01059/ES/Estratificación y tratamiento de la fibrilación auricular basada en los mecanismos de perpetuación de la arritmia/STRATIFY-AF/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ISCIII//DTS16%2F0160/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ISCIII//CIBERCV16%2F11%2F00486/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//CB16%2F11%2F00292/ES/ENFERMEDADES CARDIOVASCULARES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ISCIII//PI17%2F01106/ES/Estratificación y tratamiento de la fibrilación auricular basada en los mecanismos de perpetuación de la arritmia/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ISCIII//PI18%2F01620/ES/Modificación de los efectos pro-arrítmicos inducidos por la sobrecarga mecánica o el remodelado ventricular/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//APOSTD%2F2018%2F181/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Telecomunicación y Aplicaciones Multimedia - Institut Universitari de Telecomunicacions i Aplicacions Multimèdia es_ES
dc.description.bibliographicCitation Del-Canto, I.; Gómez-Cid, L.; Hernández-Romero, I.; Guillem Sánchez, MS.; Fernández-Santos, ME.; Atienza, F.; Such, L.... (2020). Ranolazine-mediated attenuation of mechanoelectric feedback in atrial myocyte monolayers. Frontiers in Physiology. 11:1-13. https://doi.org/10.3389/fphys.2020.00922 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fphys.2020.00922 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 13 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 11 es_ES
dc.identifier.pmid 32848863 es_ES
dc.identifier.pmcid PMC7417656 es_ES
dc.relation.pasarela S\418604 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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