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Intact RNA structurome reveals mRNA structure-mediated regulation of miRNA cleavage in vivo

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Intact RNA structurome reveals mRNA structure-mediated regulation of miRNA cleavage in vivo

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dc.contributor.author Yang, Minglei es_ES
dc.contributor.author Woolfenden, Hugh C. es_ES
dc.contributor.author Zhang, Yueying es_ES
dc.contributor.author Fang, Xiaofeng es_ES
dc.contributor.author Liu, Qi es_ES
dc.contributor.author Vigh, Maria L. es_ES
dc.contributor.author Cheema, Jitender es_ES
dc.contributor.author Yang, Xiaofei es_ES
dc.contributor.author Norris, Matthew es_ES
dc.contributor.author Yu, Sha es_ES
dc.contributor.author CARBONELL, ALBERTO es_ES
dc.contributor.author Brodersen, Peter es_ES
dc.contributor.author Wang, Jiawei es_ES
dc.contributor.author Ding, Yiliang es_ES
dc.date.accessioned 2021-05-27T03:32:43Z
dc.date.available 2021-05-27T03:32:43Z
dc.date.issued 2020-09-04 es_ES
dc.identifier.issn 0305-1048 es_ES
dc.identifier.uri http://hdl.handle.net/10251/166816
dc.description.abstract [EN] MicroRNA (miRNA)-mediated cleavage is involved in numerous essential cellular pathways. miRNAs recognize target RNAs via sequence complementarity. In addition to complementarity, in vitro and in silico studies have suggested that RNA structuremay influence the accessibility of mRNAs to miRNA-induced silencing complexes (miRISCs), thereby affecting RNA silencing. However, the regulatory mechanism of mRNA structure in miRNA cleavage remains elusive. We investigated the role of in vivo RNA secondary structure in miRNA cleavage by developing the new CAP-STRUCTURE-seq method to capture the intact mRNA structurome in Arabidopsis thaliana. This approach revealed that miRNA target sites were not structurally accessible for miRISC binding prior to cleavage in vivo. Instead, we found that the unfolding of the target site structure plays a key role in miRISC activity in vivo. We found that the single-strandedness of the two nucleotides immediately downstream of the target site, named Target Adjacent nucleotideMotif, can promotemiRNA cleavage but not miRNA binding, thus decoupling target site binding from cleavage. Our findings demonstrate that mRNA structure in vivo can modulate miRNA cleavage, providing evidence of mRNA structure-dependent regulation of biological processes. es_ES
dc.description.sponsorship Biotechnology and Biological Sciences Research Council [BB/L025000/1]; the NorwichResearch Park Science Links Seed Fund; and European Commission Horizon 2020 European Research Council, Starting Grant [680324]. Funding for open access charge: Biotechnology and Biological Sciences Research Council [BB/L025000/1]; the Norwich Research Park Science Links Seed Fund; and European Commission Horizon 2020 European Research Council, Starting Grant [680324]. es_ES
dc.language Inglés es_ES
dc.publisher Oxford University Press es_ES
dc.relation.ispartof Nucleic Acids Research es_ES
dc.rights Reconocimiento (by) es_ES
dc.title Intact RNA structurome reveals mRNA structure-mediated regulation of miRNA cleavage in vivo es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/nar/gkaa577 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/680324/EU/Investigating the role of in vivo RNA structure in RNA degradation/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UKRI//BB%2FL025000%2F1/GB/The role of RNA structures in plant response to temperature/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Yang, M.; Woolfenden, HC.; Zhang, Y.; Fang, X.; Liu, Q.; Vigh, ML.; Cheema, J.... (2020). Intact RNA structurome reveals mRNA structure-mediated regulation of miRNA cleavage in vivo. Nucleic Acids Research. 48(15):8767-8781. https://doi.org/10.1093/nar/gkaa577 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1093/nar/gkaa577 es_ES
dc.description.upvformatpinicio 8767 es_ES
dc.description.upvformatpfin 8781 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 48 es_ES
dc.description.issue 15 es_ES
dc.identifier.pmid 32652041 es_ES
dc.identifier.pmcid PMC7470952 es_ES
dc.relation.pasarela S\426453 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder UK Research and Innovation es_ES
dc.contributor.funder Norwich Research Park es_ES
dc.contributor.funder Biotechnology and Biological Sciences Research Council, Reino Unido es_ES
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