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Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor

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Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor

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dc.contributor.author Martinez-Perez, Paula es_ES
dc.contributor.author Gómez-Gómez, María Isabel es_ES
dc.contributor.author Ivanova-Angelova, Todora es_ES
dc.contributor.author Griol Barres, Amadeu es_ES
dc.contributor.author Hurtado Montañés, Juan es_ES
dc.contributor.author Bellieres, Laurent Christophe es_ES
dc.contributor.author García-Rupérez, Jaime es_ES
dc.date.accessioned 2021-05-28T03:34:04Z
dc.date.available 2021-05-28T03:34:04Z
dc.date.issued 2020-05 es_ES
dc.identifier.issn 2072-666X es_ES
dc.identifier.uri http://hdl.handle.net/10251/166911
dc.description.abstract [EN] Thrombin generation is a complex and finely regulated pathway that provokes dynamical changes of thrombin concentration in blood when a vascular injury occurs. In order to characterize the initiation phase of such process, when thrombin concentration is in the nM range, a label-free optical aptasensor is proposed here. This aptasensor combines a 1D photonic crystal structure consisting of a silicon corrugated waveguide with thrombin binding aptamers on its surface as bioreceptors. As a result, this aptasensor has been demonstrated to specifically detect thrombin concentrations ranging from 270 pM to 27 nM with an estimated detection limit of 33.5 pM and a response time of ~2 min. Furthermore, it has also been demonstrated that this aptasensor is able to continuously respond to consecutive increasing concentrations of thrombin and to detect binding events as they occur. All these features make this aptasensor a good candidate to continuously study how thrombin concentration progressively increases during the initiation phase of the coagulation cascade. es_ES
dc.description.sponsorship This research was supported by a co-financed action by the European Union through the operational program of the European Regional Development Fund (FEDER) of the Valencian Community 2014-2020, the Generalitat Valenciana through the PROMETEO project AVANTI/2019/123 and by Universitat Politecnica de Valencia through grants PAID-01-17 and the project OCUSENSOR. es_ES
dc.language Inglés es_ES
dc.publisher MDPI es_ES
dc.relation.ispartof Micromachines es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Photonic crystal es_ES
dc.subject Photonic bandgap es_ES
dc.subject Optical biosensor es_ES
dc.subject Aptasensor es_ES
dc.subject Label-free es_ES
dc.subject Thrombin es_ES
dc.subject.classification QUIMICA INORGANICA es_ES
dc.subject.classification TEORIA DE LA SEÑAL Y COMUNICACIONES es_ES
dc.title Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/mi11050464 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//PAID-01-17/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2019%2F123/ES/NANOFOTONICA AVANZADA SOBRE SILICIO (AVANTI)/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Tecnología Nanofotónica - Institut Universitari de Tecnologia Nanofotònica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Comunicaciones - Departament de Comunicacions es_ES
dc.description.bibliographicCitation Martinez-Perez, P.; Gómez-Gómez, MI.; Ivanova-Angelova, T.; Griol Barres, A.; Hurtado Montañés, J.; Bellieres, LC.; García-Rupérez, J. (2020). Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor. Micromachines. 11(5):1-12. https://doi.org/10.3390/mi11050464 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/mi11050464 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 12 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 11 es_ES
dc.description.issue 5 es_ES
dc.identifier.pmid 32354154 es_ES
dc.identifier.pmcid PMC7281654 es_ES
dc.relation.pasarela S\413125 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
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