- -

Overexpression of BvHb2, a Class 2 Non-Symbiotic Hemoglobin from Sugar Beet, Confers Drought-Induced Withering Resistance and Alters Iron Content in Tomato

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Overexpression of BvHb2, a Class 2 Non-Symbiotic Hemoglobin from Sugar Beet, Confers Drought-Induced Withering Resistance and Alters Iron Content in Tomato

Show simple item record

Files in this item

dc.contributor.author Gisbert Domenech, Maria Carmen es_ES
dc.contributor.author Timoneda, Alfonso es_ES
dc.contributor.author Porcel, R es_ES
dc.contributor.author Ros, Roc es_ES
dc.contributor.author Mulet, José Miguel es_ES
dc.date.accessioned 2021-06-01T03:31:27Z
dc.date.available 2021-06-01T03:31:27Z
dc.date.issued 2020-11 es_ES
dc.identifier.uri http://hdl.handle.net/10251/166995
dc.description.abstract [EN] Drought stress is one of the major threats to agriculture and concomitantly to food production. Tomato is one of the most important industrial crops, but its tolerance to water scarcity is very low. Traditional plant breeding has a limited margin to minimize this water requirement. In order to design novel biotechnological approaches to cope with this problem, we have screened a plant cDNA library from the halotolerant crop sugar beet (Beta vulgaris L.) for genes able to confer drought/osmotic stress tolerance to the yeast model system upon overexpression. We have identified the gene that encodes BvHb2, a class 2 non-symbiotic hemoglobin, which is present as a single copy in the sugar beet genome, expressed mainly in leaves and regulated by light and abiotic stress. We have evaluated its biotechnological potential in the model plant Arabidopsis thaliana and found that BvHb2 is able to confer drought and osmotic stress tolerance. We also generated transgenic lines of tomato (Solanum lycopersicum) overexpressing BvHb2 and found that the resulting plants are more resistant to drought-induce withering. In addition, transgenic lines overexpressing BvHb2 exhibit increased levels of iron content in leaves. Here, we show that class 2 non-symbiotic plant hemoglobins are targets to generate novel biotechnological crops tolerant to abiotic stress. The fact that these proteins are conserved in plants opens the possibility for using Non-GMO approaches, such as classical breeding, molecular breeding, or novel breeding techniques to increase drought tolerance using this protein as a target. es_ES
dc.description.sponsorship This project was funded by the project PAID-00-10 "Introduccion De Genes Relacionados Con La Tolerancia A Estres Hidrico Y Oxidativo En Distintos Materiales Que Presentan Caracteristicas Utiles Para Su Uso Como Patrones De Plantas Horticolas De Interes Agronomico". (Ref. 2726) from the Universitat Politecnica de Valencia. es_ES
dc.language Inglés es_ES
dc.publisher MDPI es_ES
dc.relation.ispartof Agronomy es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Plant hemoglobin es_ES
dc.subject Non-symbiotic es_ES
dc.subject Sugar beet es_ES
dc.subject Tomato es_ES
dc.subject Drought stress es_ES
dc.subject Iron es_ES
dc.subject Overexpression es_ES
dc.subject Beta vulgaris es_ES
dc.subject Solanum lycopersicum es_ES
dc.subject Water deprivation es_ES
dc.subject.classification GENETICA es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Overexpression of BvHb2, a Class 2 Non-Symbiotic Hemoglobin from Sugar Beet, Confers Drought-Induced Withering Resistance and Alters Iron Content in Tomato es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/agronomy10111754 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//PAID-00-10/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Gisbert Domenech, MC.; Timoneda, A.; Porcel, R.; Ros, R.; Mulet, JM. (2020). Overexpression of BvHb2, a Class 2 Non-Symbiotic Hemoglobin from Sugar Beet, Confers Drought-Induced Withering Resistance and Alters Iron Content in Tomato. Agronomy. 10(11):1-17. https://doi.org/10.3390/agronomy10111754 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/agronomy10111754 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 17 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 10 es_ES
dc.description.issue 11 es_ES
dc.identifier.eissn 2073-4395 es_ES
dc.relation.pasarela S\421582 es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
dc.description.references Mahajan, S., & Tuteja, N. (2005). Cold, salinity and drought stresses: An overview. Archives of Biochemistry and Biophysics, 444(2), 139-158. doi:10.1016/j.abb.2005.10.018 es_ES
dc.description.references Sinclair, T. R. (2011). Challenges in breeding for yield increase for drought. Trends in Plant Science, 16(6), 289-293. doi:10.1016/j.tplants.2011.02.008 es_ES
dc.description.references Burke, M., & Emerick, K. (2016). Adaptation to Climate Change: Evidence from US Agriculture. American Economic Journal: Economic Policy, 8(3), 106-140. doi:10.1257/pol.20130025 es_ES
dc.description.references Zaveri, E., Russ, J., & Damania, R. (2020). Rainfall anomalies are a significant driver of cropland expansion. Proceedings of the National Academy of Sciences, 117(19), 10225-10233. doi:10.1073/pnas.1910719117 es_ES
dc.description.references Gupta, A., Rico-Medina, A., & Caño-Delgado, A. I. (2020). The physiology of plant responses to drought. Science, 368(6488), 266-269. doi:10.1126/science.aaz7614 es_ES
dc.description.references Ashraf, M. (2010). Inducing drought tolerance in plants: Recent advances. Biotechnology Advances, 28(1), 169-183. doi:10.1016/j.biotechadv.2009.11.005 es_ES
dc.description.references Romero, C., Bellés, J. M., Vayá, J. L., Serrano, R., & Culiáñez-Macià, F. A. (1997). Expression of the yeast trehalose-6-phosphate synthase gene in transgenic tobacco plants: pleiotropic phenotypes include drought tolerance. Planta, 201(3), 293-297. doi:10.1007/s004250050069 es_ES
dc.description.references Xiao, B., Huang, Y., Tang, N., & Xiong, L. (2007). Over-expression of a LEA gene in rice improves drought resistance under the field conditions. Theoretical and Applied Genetics, 115(1), 35-46. doi:10.1007/s00122-007-0538-9 es_ES
dc.description.references Van Camp, W. (2005). Yield enhancement genes: seeds for growth. Current Opinion in Biotechnology, 16(2), 147-153. doi:10.1016/j.copbio.2005.03.002 es_ES
dc.description.references Locascio, A., Andrés-Colás, N., Mulet, J. M., & Yenush, L. (2019). Saccharomyces cerevisiae as a Tool to Investigate Plant Potassium and Sodium Transporters. International Journal of Molecular Sciences, 20(9), 2133. doi:10.3390/ijms20092133 es_ES
dc.description.references Mulet, J. M., Alemany, B., Ros, R., Calvete, J. J., & Serrano, R. (2004). Expression of a plant serine O-acetyltransferase inSaccharomyces cerevisiae confers osmotic tolerance and creates an alternative pathway for cysteine biosynthesis. Yeast, 21(4), 303-312. doi:10.1002/yea.1076 es_ES
dc.description.references Porcel, R., Bustamante, A., Ros, R., Serrano, R., & Mulet Salort, J. M. (2018). BvCOLD1: A novel aquaporin from sugar beet (Beta vulgarisL.) involved in boron homeostasis and abiotic stress. Plant, Cell & Environment, 41(12), 2844-2857. doi:10.1111/pce.13416 es_ES
dc.description.references Smagghe, B. J., Hoy, J. A., Percifield, R., Kundu, S., Hargrove, M. S., Sarath, G., … Appleby, C. A. (2009). Review: Correlations between oxygen affinity and sequence classifications of plant hemoglobins. Biopolymers, 91(12), 1083-1096. doi:10.1002/bip.21256 es_ES
dc.description.references Bogusz, D., Appleby, C. A., Landsmann, J., Dennis, E. S., Trinick, M. J., & Peacock, W. J. (1988). Functioning haemoglobin genes in non-nodulating plants. Nature, 331(6152), 178-180. doi:10.1038/331178a0 es_ES
dc.description.references Taylor, E. R., Nie, X. Z., MacGregor, A. W., & Hill, R. D. (1994). A cereal haemoglobin gene is expressed in seed and root tissues under anaerobic conditions. Plant Molecular Biology, 24(6), 853-862. doi:10.1007/bf00014440 es_ES
dc.description.references Spyrakis, F., Bruno, S., Bidon-Chanal, A., Luque, F. J., Abbruzzetti, S., Viappiani, C., … Mozzarelli, A. (2011). Oxygen binding to Arabidopsis thaliana AHb2 nonsymbiotic hemoglobin: evidence for a role in oxygen transport. IUBMB Life, 63(5), 355-362. doi:10.1002/iub.470 es_ES
dc.description.references Gupta, K. J., Hebelstrup, K. H., Mur, L. A. J., & Igamberdiev, A. U. (2011). Plant hemoglobins: Important players at the crossroads between oxygen and nitric oxide. FEBS Letters, 585(24), 3843-3849. doi:10.1016/j.febslet.2011.10.036 es_ES
dc.description.references Trevaskis, B., Watts, R. A., Andersson, C. R., Llewellyn, D. J., Hargrove, M. S., Olson, J. S., … Peacock, W. J. (1997). Two hemoglobin genes in Arabidopsis thaliana: The evolutionary origins of leghemoglobins. Proceedings of the National Academy of Sciences, 94(22), 12230-12234. doi:10.1073/pnas.94.22.12230 es_ES
dc.description.references Hill, R. D. (2012). Non-symbiotic haemoglobins—What’s happening beyond nitric oxide scavenging? AoB PLANTS, 2012. doi:10.1093/aobpla/pls004 es_ES
dc.description.references Hunt, P. W., Klok, E. J., Trevaskis, B., Watts, R. A., Ellis, M. H., Peacock, W. J., & Dennis, E. S. (2002). Increased level of hemoglobin 1 enhances survival of hypoxic stress and promotes early growth in Arabidopsis thaliana. Proceedings of the National Academy of Sciences, 99(26), 17197-17202. doi:10.1073/pnas.212648799 es_ES
dc.description.references Yang, L.-X., Wang, R.-Y., Ren, F., Liu, J., Cheng, J., & Lu, Y.-T. (2005). AtGLB1 Enhances the Tolerance of Arabidopsis to Hydrogen Peroxide Stress. Plant and Cell Physiology, 46(8), 1309-1316. doi:10.1093/pcp/pci140 es_ES
dc.description.references Hebelstrup, K. H., & Jensen, E. Ø. (2007). Expression of NO scavenging hemoglobin is involved in the timing of bolting in Arabidopsis thaliana. Planta, 227(4), 917-927. doi:10.1007/s00425-007-0667-z es_ES
dc.description.references Wang, Y., Elhiti, M., Hebelstrup, K. H., Hill, R. D., & Stasolla, C. (2011). Manipulation of hemoglobin expression affects Arabidopsis shoot organogenesis. Plant Physiology and Biochemistry, 49(10), 1108-1116. doi:10.1016/j.plaphy.2011.06.005 es_ES
dc.description.references Vigeolas, H., Hühn, D., & Geigenberger, P. (2011). Nonsymbiotic Hemoglobin-2 Leads to an Elevated Energy State and to a Combined Increase in Polyunsaturated Fatty Acids and Total Oil Content When Overexpressed in Developing Seeds of Transgenic Arabidopsis Plants. Plant Physiology, 155(3), 1435-1444. doi:10.1104/pp.110.166462 es_ES
dc.description.references Sainz, M., Pérez-Rontomé, C., Ramos, J., Mulet, J. M., James, E. K., Bhattacharjee, U., … Becana, M. (2013). Plant hemoglobins may be maintained in functional form by reduced flavins in the nuclei, and confer differential tolerance to nitro-oxidative stress. The Plant Journal, 76(5), 875-887. doi:10.1111/tpj.12340 es_ES
dc.description.references FAOSTAThttp://www.fao.org/faostat/es/#home es_ES
dc.description.references Sánchez-Rodríguez, E., Rubio-Wilhelmi, Mªm., Cervilla, L. M., Blasco, B., Rios, J. J., Rosales, M. A., … Ruiz, J. M. (2010). Genotypic differences in some physiological parameters symptomatic for oxidative stress under moderate drought in tomato plants. Plant Science, 178(1), 30-40. doi:10.1016/j.plantsci.2009.10.001 es_ES
dc.description.references Gur, A., & Zamir, D. (2004). Unused Natural Variation Can Lift Yield Barriers in Plant Breeding. PLoS Biology, 2(10), e245. doi:10.1371/journal.pbio.0020245 es_ES
dc.description.references McCormick, S., Niedermeyer, J., Fry, J., Barnason, A., Horsch, R., & Fraley, R. (1986). Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens. Plant Cell Reports, 5(2), 81-84. doi:10.1007/bf00269239 es_ES
dc.description.references Gisbert, C., Rus, A. M., Boları́n, M. C., López-Coronado, J. M., Arrillaga, I., Montesinos, C., … Moreno, V. (2000). The Yeast HAL1 Gene Improves Salt Tolerance of Transgenic Tomato. Plant Physiology, 123(1), 393-402. doi:10.1104/pp.123.1.393 es_ES
dc.description.references Römer, S., Fraser, P. D., Kiano, J. W., Shipton, C. A., Misawa, N., Schuch, W., & Bramley, P. M. (2000). Elevation of the provitamin A content of transgenic tomato plants. Nature Biotechnology, 18(6), 666-669. doi:10.1038/76523 es_ES
dc.description.references Muir, S. R., Collins, G. J., Robinson, S., Hughes, S., Bovy, A., Ric De Vos, C. H., … Verhoeyen, M. E. (2001). Overexpression of petunia chalcone isomerase in tomato results in fruit containing increased levels of flavonols. Nature Biotechnology, 19(5), 470-474. doi:10.1038/88150 es_ES
dc.description.references Schijlen, E., Ric de Vos, C. H., Jonker, H., van den Broeck, H., Molthoff, J., van Tunen, A., … Bovy, A. (2006). Pathway engineering for healthy phytochemicals leading to the production of novel flavonoids in tomato fruit. Plant Biotechnology Journal, 4(4), 433-444. doi:10.1111/j.1467-7652.2006.00192.x es_ES
dc.description.references Fischhoff, D. A., Bowdish, K. S., Perlak, F. J., Marrone, P. G., McCormick, S. M., Niedermeyer, J. G., … Fraley, R. T. (1987). Insect Tolerant Transgenic Tomato Plants. Nature Biotechnology, 5(8), 807-813. doi:10.1038/nbt0887-807 es_ES
dc.description.references KIM, J. W. (1994). Disease Resistance in Tobacco and Tomato Plants Transformed with the Tomato Spotted Wilt Virus Nucleocapsid Gene. Plant Disease, 78(6), 615. doi:10.1094/pd-78-0615 es_ES
dc.description.references Fillatti, J. J., Kiser, J., Rose, R., & Comai, L. (1987). Efficient Transfer of a Glyphosate Tolerance Gene into Tomato Using a Binary Agrobacterium Tumefaciens Vector. Nature Biotechnology, 5(7), 726-730. doi:10.1038/nbt0787-726 es_ES
dc.description.references Z.-Q., Z., F.-Q., C., Y.-X., L., & G.-X., J. (2002). Transformation of tomato with the BADH gene from Atriplex improves salt tolerance. Plant Cell Reports, 21(2), 141-146. doi:10.1007/s00299-002-0489-1 es_ES
dc.description.references Roy, R., Purty, R. S., Agrawal, V., & Gupta, S. C. (2005). Transformation of tomato cultivar ‘Pusa Ruby’ with bspA gene from Populus tremula for drought tolerance. Plant Cell, Tissue and Organ Culture, 84(1), 56-68. doi:10.1007/s11240-005-9000-3 es_ES
dc.description.references Sheehy, R. E., Kramer, M., & Hiatt, W. R. (1988). Reduction of polygalacturonase activity in tomato fruit by antisense RNA. Proceedings of the National Academy of Sciences, 85(23), 8805-8809. doi:10.1073/pnas.85.23.8805 es_ES
dc.description.references Klee, H. J. (1993). Ripening Physiology of Fruit from Transgenic Tomato (Lycopersicon esculentum) Plants with Reduced Ethylene Synthesis. Plant Physiology, 102(3), 911-916. doi:10.1104/pp.102.3.911 es_ES
dc.description.references Klee, H. J., Hayford, M. B., Kretzmer, K. A., Barry, G. F., & Kishore, G. M. (1991). Control of ethylene synthesis by expression of a bacterial enzyme in transgenic tomato plants. The Plant Cell, 3(11), 1187-1193. doi:10.1105/tpc.3.11.1187 es_ES
dc.description.references Arrillaga, I., Gil-Mascarell, R., Gisbert, C., Sales, E., Montesinos, C., Serrano, R., & Moreno, V. (1998). Expression of the yeast HAL2 gene in tomato increases the in vitro salt tolerance of transgenic progenies. Plant Science, 136(2), 219-226. doi:10.1016/s0168-9452(98)00122-8 es_ES
dc.description.references García-Abellan, J. O., Egea, I., Pineda, B., Sanchez-Bel, P., Belver, A., Garcia-Sogo, B., … Bolarin, M. C. (2014). Heterologous expression of the yeastHAL5gene in tomato enhances salt tolerance by reducing shoot Na+accumulation in the long term. Physiologia Plantarum, 152(4), 700-713. doi:10.1111/ppl.12217 es_ES
dc.description.references Safdar, N., Yasmeen, A., & Mirza, B. (2010). An insight into functional genomics of transgenic lines of tomato cv Rio Grande harbouring yeast halotolerance genes. Plant Biology, 13(4), 620-631. doi:10.1111/j.1438-8677.2010.00412.x es_ES
dc.description.references Sade, N., Vinocur, B. J., Diber, A., Shatil, A., Ronen, G., Nissan, H., … Moshelion, M. (2008). Improving plant stress tolerance and yield production: is the tonoplast aquaporin SlTIP2;2 a key to isohydric to anisohydric conversion? New Phytologist, 181(3), 651-661. doi:10.1111/j.1469-8137.2008.02689.x es_ES
dc.description.references Goel, D., Singh, A. K., Yadav, V., Babbar, S. B., & Bansal, K. C. (2010). Overexpression of osmotin gene confers tolerance to salt and drought stresses in transgenic tomato (Solanum lycopersicum L.). Protoplasma, 245(1-4), 133-141. doi:10.1007/s00709-010-0158-0 es_ES
dc.description.references Goel, D., Singh, A. K., Yadav, V., Babbar, S. B., Murata, N., & Bansal, K. C. (2011). Transformation of tomato with a bacterial codA gene enhances tolerance to salt and water stresses. Journal of Plant Physiology, 168(11), 1286-1294. doi:10.1016/j.jplph.2011.01.010 es_ES
dc.description.references Mishra, K. B., Iannacone, R., Petrozza, A., Mishra, A., Armentano, N., La Vecchia, G., … Nedbal, L. (2012). Engineered drought tolerance in tomato plants is reflected in chlorophyll fluorescence emission. Plant Science, 182, 79-86. doi:10.1016/j.plantsci.2011.03.022 es_ES
dc.description.references Seo, Y. S., Choi, J. Y., Kim, S. J., Kim, E. Y., Shin, J. S., & Kim, W. T. (2012). Constitutive expression of CaRma1H1, a hot pepper ER-localized RING E3 ubiquitin ligase, increases tolerance to drought and salt stresses in transgenic tomato plants. Plant Cell Reports, 31(9), 1659-1665. doi:10.1007/s00299-012-1278-0 es_ES
dc.description.references Muñoz-Mayor, A., Pineda, B., Garcia-Abellán, J. O., Antón, T., Garcia-Sogo, B., Sanchez-Bel, P., … Bolarin, M. C. (2012). Overexpression of dehydrin tas14 gene improves the osmotic stress imposed by drought and salinity in tomato. Journal of Plant Physiology, 169(5), 459-468. doi:10.1016/j.jplph.2011.11.018 es_ES
dc.description.references Zhang, X., Zou, Z., Gong, P., Zhang, J., Ziaf, K., Li, H., … Ye, Z. (2010). Over-expression of microRNA169 confers enhanced drought tolerance to tomato. Biotechnology Letters, 33(2), 403-409. doi:10.1007/s10529-010-0436-0 es_ES
dc.description.references Kanhonou, R., Serrano, R., & Ros Palau, R. (2001). Plant Molecular Biology, 47(5), 571-579. doi:10.1023/a:1012227913356 es_ES
dc.description.references Rosa Téllez, S., Kanhonou, R., Castellote Bellés, C., Serrano, R., Alepuz, P., & Ros, R. (2020). RNA-Binding Proteins as Targets to Improve Salt Stress Tolerance in Crops. Agronomy, 10(2), 250. doi:10.3390/agronomy10020250 es_ES
dc.description.references Brunelli, J. P., & Pall, M. L. (1993). A series of yeast shuttle vectors for expression of cDNAs and other DNA sequences. Yeast, 9(12), 1299-1308. doi:10.1002/yea.320091203 es_ES
dc.description.references Gietz, D., Jean, A. S., Woods, R. A., & Schiestl, R. H. (1992). Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Research, 20(6), 1425-1425. doi:10.1093/nar/20.6.1425 es_ES
dc.description.references Hoeberichts, F. A., Perez-Valle, J., Montesinos, C., Mulet, J. M., Planes, M. D., Hueso, G., … Serrano, R. (2010). The role of K+ and H+ transport systems during glucose- and H2O2-induced cell death in Saccharomyces cerevisiae. Yeast, 27(9), 713-725. doi:10.1002/yea.1767 es_ES
dc.description.references Sayle, R. (1995). RASMOL: biomolecular graphics for all. Trends in Biochemical Sciences, 20(9), 374-376. doi:10.1016/s0968-0004(00)89080-5 es_ES
dc.description.references Hoy, J. A., & Hargrove, M. S. (2008). The structure and function of plant hemoglobins. Plant Physiology and Biochemistry, 46(3), 371-379. doi:10.1016/j.plaphy.2007.12.016 es_ES
dc.description.references Kay, R., Chan, A., Daly, M., & McPherson, J. (1987). Duplication of CaMV 35 S Promoter Sequences Creates a Strong Enhancer for Plant Genes. Science, 236(4806), 1299-1302. doi:10.1126/science.236.4806.1299 es_ES
dc.description.references Livak, K. J., & Schmittgen, T. D. (2001). Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods, 25(4), 402-408. doi:10.1006/meth.2001.1262 es_ES
dc.description.references Bissoli, G., Niñoles, R., Fresquet, S., Palombieri, S., Bueso, E., Rubio, L., … Serrano, R. (2012). Peptidyl-prolyl cis-trans isomerase ROF2 modulates intracellular pH homeostasis in Arabidopsis. The Plant Journal, 70(4), 704-716. doi:10.1111/j.1365-313x.2012.04921.x es_ES
dc.description.references Witte, C.-P., No�l, L., Gielbert, J., Parker, J., & Romeis, T. (2004). Rapid one-step protein purification from plant material using the eight-amino acid StrepII epitope. Plant Molecular Biology, 55(1), 135-147. doi:10.1007/s11103-004-0501-y es_ES
dc.description.references Saporta, R., Bou, C., Frías, V., & Mulet, J. (2019). A Method for a Fast Evaluation of the Biostimulant Potential of Different Natural Extracts for Promoting Growth or Tolerance against Abiotic Stress. Agronomy, 9(3), 143. doi:10.3390/agronomy9030143 es_ES
dc.description.references Trujillo-Moya, C., & Gisbert, C. (2012). The influence of ethylene and ethylene modulators on shoot organogenesis in tomato. Plant Cell, Tissue and Organ Culture (PCTOC), 111(1), 41-48. doi:10.1007/s11240-012-0168-z es_ES
dc.description.references Koncz, C., & Schell, J. (1986). The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector. Molecular and General Genetics MGG, 204(3), 383-396. doi:10.1007/bf00331014 es_ES
dc.description.references Ríos, G., Cabedo, M., Rull, B., Yenush, L., Serrano, R., & Mulet, J. M. (2013). Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response. FEMS Yeast Research, 13(1), 97-106. doi:10.1111/1567-1364.12013 es_ES
dc.description.references Citation for the Real Statistics Software or Website. Real Statistics Using Excelhttp://www.real-statistics.com/appendix/citation-real-statistics-software-website/ es_ES
dc.description.references Tukey, J. W. (1949). Comparing Individual Means in the Analysis of Variance. Biometrics, 5(2), 99. doi:10.2307/3001913 es_ES
dc.description.references Forment, J., Naranjo, M. A., Roldan, M., Serrano, R., & Vicente, O. (2002). Expression of Arabidopsis SR-like splicing proteins confers salt tolerance to yeast and transgenic plants. The Plant Journal, 30(5), 511-519. doi:10.1046/j.1365-313x.2002.01311.x es_ES
dc.description.references Hunt, P. W., Watts, R. A., Trevaskis, B., Llewelyn, D. J., Burnell, J., Dennis, E. S., & Peacock, W. J. (2001). Plant Molecular Biology, 47(5), 677-692. doi:10.1023/a:1012440926982 es_ES
dc.description.references Dohm, J. C., Minoche, A. E., Holtgräwe, D., Capella-Gutiérrez, S., Zakrzewski, F., Tafer, H., … Himmelbauer, H. (2013). The genome of the recently domesticated crop plant sugar beet (Beta vulgaris). Nature, 505(7484), 546-549. doi:10.1038/nature12817 es_ES
dc.description.references Hebelstrup, K. H., Igamberdiev, A. U., & Hill, R. D. (2007). Metabolic effects of hemoglobin gene expression in plants. Gene, 398(1-2), 86-93. doi:10.1016/j.gene.2007.01.039 es_ES
dc.description.references Hargrove, M. S., Brucker, E. A., Stec, B., Sarath, G., Arredondo-Peter, R., Klucas, R. V., … Phillips, G. N. (2000). Crystal structure of a nonsymbiotic plant hemoglobin. Structure, 8(9), 1005-1014. doi:10.1016/s0969-2126(00)00194-5 es_ES
dc.description.references Leiva-Eriksson, N., Pin, P. A., Kraft, T., Dohm, J. C., Minoche, A. E., Himmelbauer, H., & Bülow, L. (2014). Differential Expression Patterns of Non-Symbiotic Hemoglobins in Sugar Beet (Beta vulgaris ssp. vulgaris). Plant and Cell Physiology, 55(4), 834-844. doi:10.1093/pcp/pcu027 es_ES
dc.description.references NARANJO, M. A., FORMENT, J., ROLDAN, M., SERRANO, R., & VICENTE, O. (2006). Overexpression of Arabidopsis thaliana LTL1, a salt-induced gene encoding a GDSL-motif lipase, increases salt tolerance in yeast and transgenic plants. Plant, Cell and Environment, 29(10), 1890-1900. doi:10.1111/j.1365-3040.2006.01565.x es_ES
dc.description.references Rausell, A., Kanhonou, R., Yenush, L., Serrano, R., & Ros, R. (2003). The translation initiation factor eIF1A is an important determinant in the tolerance to NaCl stress in yeast and plants. The Plant Journal, 34(3), 257-267. doi:10.1046/j.1365-313x.2003.01719.x es_ES
dc.description.references Rus, A. M., Estañ, M. T., Gisbert, C., Garcia-Sogo, B., Serrano, R., Caro, M., … Bolarín, M. C. (2001). Expressing the yeast HAL1 gene in tomato increases fruit yield and enhances K+ /Na+ selectivity under salt stress. Plant, Cell & Environment, 24(8), 875-880. doi:10.1046/j.1365-3040.2001.00719.x es_ES
dc.description.references Hoogewijs, D., Dewilde, S., Vierstraete, A., Moens, L., & Vinogradov, S. N. (2012). A Phylogenetic Analysis of the Globins in Fungi. PLoS ONE, 7(2), e31856. doi:10.1371/journal.pone.0031856 es_ES
dc.description.references Bai, X., Long, J., He, X., Yan, J., Chen, X., Tan, Y., … Xu, H. (2016). Overexpression of spinach non-symbiotic hemoglobin in Arabidopsis resulted in decreased NO content and lowered nitrate and other abiotic stresses tolerance. Scientific Reports, 6(1). doi:10.1038/srep26400 es_ES
dc.description.references Evangelou, E., Tsadilas, C., Tserlikakis, N., Tsitouras, A., & Kyritsis, A. (2016). Water Footprint of Industrial Tomato Cultivations in the Pinios River Basin: Soil Properties Interactions. Water, 8(11), 515. doi:10.3390/w8110515 es_ES
dc.description.references (2012). The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 485(7400), 635-641. doi:10.1038/nature11119 es_ES
dc.description.references Chen, Y., & Barak, P. (1982). Iron Nutrition of Plants in Calcareous Soils. Advances in Agronomy Volume 35, 217-240. doi:10.1016/s0065-2113(08)60326-0 es_ES
dc.description.references Dutt, M., Dhekney, S. A., Soriano, L., Kandel, R., & Grosser, J. W. (2014). Temporal and spatial control of gene expression in horticultural crops. Horticulture Research, 1(1). doi:10.1038/hortres.2014.47 es_ES
dc.subject.ods 08.- Fomentar el crecimiento económico sostenido, inclusivo y sostenible, el empleo pleno y productivo, y el trabajo decente para todos es_ES
dc.subject.ods 06.- Garantizar la disponibilidad y la gestión sostenible del agua y el saneamiento para todos es_ES
dc.subject.ods 03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades es_ES
dc.subject.ods 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible es_ES
dc.subject.ods 15.- Proteger, restaurar y promover la utilización sostenible de los ecosistemas terrestres, gestionar de manera sostenible los bosques, combatir la desertificación y detener y revertir la degradación de la tierra, y frenar la pérdida de diversidad biológica es_ES
dc.subject.ods 13.- Tomar medidas urgentes para combatir el cambio climático y sus efectos es_ES
dc.subject.ods 01.- Erradicar la pobreza en todas sus formas en todo el mundo es_ES
dc.subject.ods 12.- Garantizar las pautas de consumo y de producción sostenibles es_ES


This item appears in the following Collection(s)

Show simple item record