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Nitrite Reductase 1 Is a Target of Nitric Oxide-Mediated Post-Translational Modifications and Controls Nitrogen Flux and Growth in Arabidopsis

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Nitrite Reductase 1 Is a Target of Nitric Oxide-Mediated Post-Translational Modifications and Controls Nitrogen Flux and Growth in Arabidopsis

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dc.contributor.author Costa-Broseta, Álvaro es_ES
dc.contributor.author Castillo López Del Toro, Mª Cruz es_ES
dc.contributor.author LEON RAMOS, JOSE es_ES
dc.date.accessioned 2021-07-17T03:34:46Z
dc.date.available 2021-07-17T03:34:46Z
dc.date.issued 2020-10 es_ES
dc.identifier.uri http://hdl.handle.net/10251/169421
dc.description.abstract [EN] Plant growth is the result of the coordinated photosynthesis-mediated assimilation of oxidized forms of C, N and S. Nitrate is the predominant N source in soils and its reductive assimilation requires the successive activities of soluble cytosolic NADH-nitrate reductases (NR) and plastid stroma ferredoxin-nitrite reductases (NiR) allowing the conversion of nitrate to nitrite and then to ammonium. However, nitrite, instead of being reduced to ammonium in plastids, can be reduced to nitric oxide (NO) in mitochondria, through a process that is relevant under hypoxic conditions, or in the cytoplasm, through a side-reaction catalyzed by NRs. We use a loss-of-function approach, based on CRISPR/Cas9-mediated genetic edition, and gain-of-function, using transgenic overexpressing HA-tagged Arabidopsis NiR1 to characterize the role of this enzyme in controlling plant growth, and to propose that the NO-related post-translational modifications, by S-nitrosylation of key C residues, might inactivate NiR1 under stress conditions. NiR1 seems to be a key target in regulating nitrogen assimilation and NO homeostasis, being relevant to the control of both plant growth and performance under stress conditions. Because most higher plants including crops have a single NiR, the modulation of its function might represent a relevant target for agrobiotechnological purposes. es_ES
dc.description.sponsorship This research was funded by BIO2014-56067-P and BIO2017-82945-P grants from the Spanish Ministry of Economy, Industry and Competitiveness and Fondo Europeo de Desarrollo Regional (FEDER) funds. es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof International Journal of Molecular Sciences es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject CRISPR es_ES
dc.subject Cysteine S-nitrosylation es_ES
dc.subject Nitrate assimilation es_ES
dc.subject Nitric oxide es_ES
dc.subject Nitrite reductase es_ES
dc.subject Tyrosine nitration es_ES
dc.subject Plant growth es_ES
dc.subject Ubiquitylation es_ES
dc.title Nitrite Reductase 1 Is a Target of Nitric Oxide-Mediated Post-Translational Modifications and Controls Nitrogen Flux and Growth in Arabidopsis es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/ijms21197270 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-82945-P/ES/TOLERANCIA AL OXIGENO Y AL OXIDO NITRICO TRAS HIPOXIA EN ARABIDOPSIS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2014-56067-P/ES/CONTROL DE LA PRODUCCION, PERCEPCION Y SEÑALIZACION DE NO POR MODIFICACIONES POSTRADUCCIONALES Y PROTEOLISIS DIRIGIDA POR LA SECUENCIA AMINOTERMINAL/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Costa-Broseta, Á.; Castillo López Del Toro, MC.; Leon Ramos, J. (2020). Nitrite Reductase 1 Is a Target of Nitric Oxide-Mediated Post-Translational Modifications and Controls Nitrogen Flux and Growth in Arabidopsis. International Journal of Molecular Sciences. 21(19):1-13. https://doi.org/10.3390/ijms21197270 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/ijms21197270 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 13 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 21 es_ES
dc.description.issue 19 es_ES
dc.identifier.eissn 1422-0067 es_ES
dc.identifier.pmid 33019636 es_ES
dc.identifier.pmcid PMC7582248 es_ES
dc.relation.pasarela S\433248 es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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