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A cell-free approach with a supporting biomaterial in the form of dispersed microspheres induces hyaline cartilage formation in a rabbit knee model

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A cell-free approach with a supporting biomaterial in the form of dispersed microspheres induces hyaline cartilage formation in a rabbit knee model

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dc.contributor.author Zurriaga Carda, Javier es_ES
dc.contributor.author Lastra, Maria L. es_ES
dc.contributor.author Antolinos-Turpin, Carmen M. es_ES
dc.contributor.author Morales-Román, Rosa M. es_ES
dc.contributor.author Sancho-Tello, María es_ES
dc.contributor.author Perea-Ruiz, Sofía es_ES
dc.contributor.author Milián, Lara es_ES
dc.contributor.author Fernández, Juan M. es_ES
dc.contributor.author Cortizo, Ana M. es_ES
dc.contributor.author Carda, Carmen es_ES
dc.contributor.author Gallego-Ferrer, Gloria es_ES
dc.contributor.author Gómez Ribelles, José Luís es_ES
dc.date.accessioned 2021-07-21T03:31:13Z
dc.date.available 2021-07-21T03:31:13Z
dc.date.issued 2020-05 es_ES
dc.identifier.issn 1552-4973 es_ES
dc.identifier.uri http://hdl.handle.net/10251/169640
dc.description.abstract [EN] The objective of this study was to test a regenerative medicine strategy for the regeneration of articular cartilage. This approach combines microfracture of the subchondral bone with the implant at the site of the cartilage defect of a supporting biomaterial in the form of microspheres aimed at creating an adequate biomechanical environment for the differentiation of the mesenchymal stem cells that migrate from the bone marrow. The possible inflammatory response to these biomaterials was previously studied by means of the culture of RAW264.7 macrophages. The microspheres were implanted in a 3¿mm-diameter defect in the trochlea of the femoral condyle of New Zealand rabbits, covering them with a poly(l-lactic acid) (PLLA) membrane manufactured by electrospinning. Experimental groups included a group where exclusively PLLA microspheres were implanted, another group where a mixture of 50/50 microspheres of PLLA (hydrophobic and rigid) and others of chitosan (a hydrogel) were used, and a third group used as a control where no material was used and only the membrane was covering the defect. The histological characteristics of the regenerated tissue have been evaluated 3 months after the operation. We found that during the regeneration process the microspheres, and the membrane covering them, are displaced by the neoformed tissue in the regeneration space toward the subchondral bone region, leaving room for the formation of a tissue with the characteristics of hyaline cartilage. es_ES
dc.description.sponsorship Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA), Universidad Nacional de La Plata, Grant/Award Number: 11/X643; Agencia Estatal de Investigación/Fondo Europeo de Desarrollo Regional de la Unión Europea, Grant/Award Number: MAT2016-76039-C4-1 2-R; Spanish Ministry of Economy and Competitiveness (MINECO) es_ES
dc.language Inglés es_ES
dc.publisher John Wiley & Sons es_ES
dc.relation.ispartof Journal of Biomedical Materials Research Part B Applied Biomaterials es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Articular cartilage regeneration es_ES
dc.subject Cartilage engineering es_ES
dc.subject Chitosan es_ES
dc.subject Microspheres es_ES
dc.subject Polylactide es_ES
dc.subject Rabbit knee model es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title A cell-free approach with a supporting biomaterial in the form of dispersed microspheres induces hyaline cartilage formation in a rabbit knee model es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1002/jbm.b.34490 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UNLP//11%2FX643/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2016-76039-C4-1-R/ES/BIOMATERIALES PIEZOELECTRICOS PARA LA DIFERENCIACION CELULAR EN INTERFASES CELULA-MATERIAL ELECTRICAMENTE ACTIVAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Zurriaga Carda, J.; Lastra, ML.; Antolinos-Turpin, CM.; Morales-Román, RM.; Sancho-Tello, M.; Perea-Ruiz, S.; Milián, L.... (2020). A cell-free approach with a supporting biomaterial in the form of dispersed microspheres induces hyaline cartilage formation in a rabbit knee model. Journal of Biomedical Materials Research Part B Applied Biomaterials. 108(4):1428-1438. https://doi.org/10.1002/jbm.b.34490 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1002/jbm.b.34490 es_ES
dc.description.upvformatpinicio 1428 es_ES
dc.description.upvformatpfin 1438 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 108 es_ES
dc.description.issue 4 es_ES
dc.identifier.pmid 31520507 es_ES
dc.relation.pasarela S\405032 es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Universidad Nacional de La Plata, Argentina es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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dc.subject.ods 03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades es_ES


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