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Structure, morphology, adhesion and in vitro biological evaluation of antibacterial multi-layer HA-Ag/SiO2/TiN/Ti coatings obtained by RF magnetron sputtering for biomedical applications

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Structure, morphology, adhesion and in vitro biological evaluation of antibacterial multi-layer HA-Ag/SiO2/TiN/Ti coatings obtained by RF magnetron sputtering for biomedical applications

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dc.contributor.author Lenis, J. A. es_ES
dc.contributor.author Rico Tortosa, Patricia María es_ES
dc.contributor.author Gómez Ribelles, José Luís es_ES
dc.contributor.author Pacha-Olivenza, M. A. es_ES
dc.contributor.author González-Martín, M. L. es_ES
dc.contributor.author Bolívar, F. J. es_ES
dc.date.accessioned 2021-09-09T03:35:46Z
dc.date.available 2021-09-09T03:35:46Z
dc.date.issued 2020-11 es_ES
dc.identifier.uri http://hdl.handle.net/10251/171691
dc.description.abstract [EN] Biocompatible and antibacterial multi-layer coatings of hydroxyapatite (HA)-Ag/SiO2/TiN/Ti were obtained on the Ti-6Al-4V alloy, by means of the magnetron sputtering technique. During characterization of the coatings, the chemical composition was evaluated by energy dispersive X-ray spectroscopy and the phase analysis was carried out by X-ray diffraction. The morphology of the coatings was observed by field emission scanning electron microscopy, while transmission electron microscopy was used to appreciate their structure. The adhesion of the coatings to the substrate was evaluated by micro scratch test. The in vitro biological response was evaluated in terms of cytotoxicity, adhesion and differentiation of mouse mesenchymal stem cells, as well as adhesion and bacterial viability of Staphylococcus aureus strain. Through the compositional study carried out, the deposition of the HA phase was verified, with a Ca/P ratio close to 1.67 and the characteristic diffraction peaks of this compound. The structural study of the coatings evidenced the obtention of multi-layer architectures. The use of an intermediate SiO2/TiN/Ti trilayer was found to improve adhesion between HA-Ag and the substrate by 84%. Finally, the in vitro biological tests carried out indicated a potentially non-toxic character in the coatings. Additionally, an antibacterial effect was registered at low concentrations of Ag (< 0.25 mg/L). es_ES
dc.description.sponsorship We thank the University of Antioquia, the Centro de Investigacion, Innovacion y Desarrollo de materiales (CIDEMAT) group, the Departamento Administrativo de Ciencia, Tecnologia e Innovacion (COLCIENCIAS) for financing the Project 15-1696, the scholarship program of Enlazamundos, the Agencia de Educacion Superior de Medellin (Sapiencia), JLGR acknowledges financial support from the Spanish Ministry of Economy and Competitiveness (MINECO) through the project MAT2016-76039-C4-1-R and MAT 2015-63974-C4-3-R (AEI/FEDER, UE) (including the FEDER financial support). PR acknowledges support from the Spanish Ministry of Science, Innovation and Universities (RTI2018-096794), and Fondo Europeo de Desarrollo Regional (FEDER). CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER Actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund, M-ERA.NET PCIN-2016-146 and RTI2018-096862-B-I00, Spanish "Junta de Extremadura" for the projects IB16117, TE-0016-18 and GR18153. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier BV es_ES
dc.relation.ispartof Materials Science and Engineering C: Materials for Biological Applications (Online) es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Magnetron sputtering es_ES
dc.subject Multi-layer coating es_ES
dc.subject Hydroxyapatite es_ES
dc.subject Structure es_ES
dc.subject Interface es_ES
dc.subject In vitro biological properties es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title Structure, morphology, adhesion and in vitro biological evaluation of antibacterial multi-layer HA-Ag/SiO2/TiN/Ti coatings obtained by RF magnetron sputtering for biomedical applications es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.msec.2020.111268 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096794-B-I00/ES/DISEÑO DE MICROENTORNOS CELULARES PARA PROMOVER LA MECANOTRANSDUCCION SINERGICA DE CANALES DE IONES E INTEGRINAS./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2015-63974-C4-3-R/ES/CARACTERIZACION SUPERFICIAL Y ADHESION MICROBIANA DE MATERIALES BIODEGRADABLES Y BIOREABSORBIBLES BASE MAGNESIO PARA LA REPARACION DEL HUESO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI//PCIN-2016-146/ES/SIGNALING IMPLANT/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096862-B-I00/ES/RESPUESTA DE LAS PROPIEDADES ADHESIVAS DE PATOGENOS A LA LIBERACION CONTROLADA DE COMPUESTOS ACTIVOS NATURALES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Junta de Extremadura//IB16117/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Junta de Extremadura//TE-0016-18/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Junta de Extremadura//GR18153/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2016-76039-C4-1-R/ES/BIOMATERIALES PIEZOELECTRICOS PARA LA DIFERENCIACION CELULAR EN INTERFASES CELULA-MATERIAL ELECTRICAMENTE ACTIVAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/COLCIENCIAS//15-1696/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Lenis, JA.; Rico Tortosa, PM.; Gómez Ribelles, JL.; Pacha-Olivenza, MA.; González-Martín, ML.; Bolívar, FJ. (2020). Structure, morphology, adhesion and in vitro biological evaluation of antibacterial multi-layer HA-Ag/SiO2/TiN/Ti coatings obtained by RF magnetron sputtering for biomedical applications. Materials Science and Engineering C: Materials for Biological Applications (Online). 116:1-13. https://doi.org/10.1016/j.msec.2020.111268 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.msec.2020.111268 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 13 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 116 es_ES
dc.identifier.eissn 1873-0191 es_ES
dc.identifier.pmid 32806245 es_ES
dc.relation.pasarela S\433280 es_ES
dc.contributor.funder Junta de Extremadura es_ES
dc.contributor.funder Universidad de Antioquia es_ES
dc.contributor.funder Universidad de Extremadura es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Departamento Administrativo de Ciencia, Tecnología e Innovación, Colombia es_ES
dc.contributor.funder Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina es_ES
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dc.subject.ods 03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades es_ES


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