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Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Candidatus Liberibacter Solanacearum from Different Host Plants in Spain

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Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Candidatus Liberibacter Solanacearum from Different Host Plants in Spain

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dc.contributor.author Ruiz-Padilla, Ana es_ES
dc.contributor.author Redondo, Cristina es_ES
dc.contributor.author Asensio, Adrian es_ES
dc.contributor.author Garita-Cambronero, Jerson es_ES
dc.contributor.author Martinez, Carmen es_ES
dc.contributor.author Perez-Padilla, Verónica es_ES
dc.contributor.author Marquinez, Raquel es_ES
dc.contributor.author Collar, Jesus es_ES
dc.contributor.author Garcia-Mendez, Eva es_ES
dc.contributor.author Alfaro Fernández, Ana Olvido es_ES
dc.contributor.author Asensio-S-Manzanera, Carmen es_ES
dc.contributor.author Palomo, José Luis es_ES
dc.contributor.author Siverio, Felipe es_ES
dc.contributor.author De Leon, Leandro es_ES
dc.contributor.author Cubero, Jaime es_ES
dc.date.accessioned 2021-09-10T03:30:50Z
dc.date.available 2021-09-10T03:30:50Z
dc.date.issued 2020-09-21 es_ES
dc.identifier.uri http://hdl.handle.net/10251/171996
dc.description.abstract [EN] Liberibacteris a bacterial group causing different diseases and disorders in plants. Among liberibacters,CandidatusLiberibacter solanaceraum (CLso) produces disorders in several species mainly within Apiaceae and Solanaceae families. CLso isolates are usually grouped in defined haplotypes according to single nucleotide polymorphisms in genes associated with ribosomal elements. In order to characterize more precisely isolates of CLso identified in potato in Spain, a Multilocus Sequence Analysis (MLSA) was applied. This methodology was validated by a complete analysis of ten housekeeping genes that showed an absence of positive selection and a nearly neutral mechanism for their evolution. Most of the analysis performed with single housekeeping genes, as well as MLSA, grouped together isolates of CLso detected in potato crops in Spain within the haplotype E, undistinguishable from those infecting carrots, parsnips or celery. Moreover, the information from these housekeeping genes was used to estimate the evolutionary divergence among the different CLso by using the concatenated sequences of the genes assayed. Data obtained on the divergence among CLso haplotypes support the hypothesis of evolutionary events connected with different hosts, in different geographic areas, and possibly associated with different vectors. Our results demonstrate the absence in Spain of CLso isolates molecularly classified as haplotypes A and B, traditionally considered causal agents of zebra chip in potato, as well as the uncertain possibility of the present haplotype to produce major disease outbreaks in potato that may depend on many factors that should be further evaluated in future works es_ES
dc.description.sponsorship This research was funded by Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), grant numbers AT2016-007 and RTA2014-00008-C04-03-E, co-financed by FEDER. es_ES
dc.language Inglés es_ES
dc.publisher MDPI es_ES
dc.relation.ispartof Microorganisms es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Liberibacter es_ES
dc.subject Zebra chip es_ES
dc.subject MLSA es_ES
dc.subject Potato es_ES
dc.subject Carrot es_ES
dc.subject Parsnip es_ES
dc.subject Celery es_ES
dc.subject Citrus es_ES
dc.subject HLB es_ES
dc.title Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Candidatus Liberibacter Solanacearum from Different Host Plants in Spain es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/microorganisms8091446 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/INIA//AT2016-007/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//E-RTA2014-00010-C02-02/ES/Epidemiología del STV y su implicación en el "síndrome del falso PepMV"/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani es_ES
dc.description.bibliographicCitation Ruiz-Padilla, A.; Redondo, C.; Asensio, A.; Garita-Cambronero, J.; Martinez, C.; Perez-Padilla, V.; Marquinez, R.... (2020). Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Candidatus Liberibacter Solanacearum from Different Host Plants in Spain. Microorganisms. 8(9):1-19. https://doi.org/10.3390/microorganisms8091446 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/microorganisms8091446 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 19 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.description.issue 9 es_ES
dc.identifier.eissn 2076-2607 es_ES
dc.identifier.pmid 32967215 es_ES
dc.identifier.pmcid PMC7565762 es_ES
dc.relation.pasarela S\432618 es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder INSTITUTO NACIONAL DE INV. Y TECNOL. AGRARIA Y ALIMENTARIA es_ES
dc.contributor.funder Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria es_ES
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